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Characterization Of Afn1, A Gene Associated With Cereal Grain Germination, Tenzin Tsewang, Russell Johnson
Characterization Of Afn1, A Gene Associated With Cereal Grain Germination, Tenzin Tsewang, Russell Johnson
Undergraduate Research Symposium (UGRS)
The AFN1 gene is transiently expressed in germinating oat grains. As AFN1 is not expressed in dormant oat grains during imbibition, we hypothesize that AFN1 may be involved in stimulating the germination process. Sequence analysis of an AFN1 cDNA clone indicates that the AFN1 polypeptide is similar to a previously identified abscisic acid (ABA) glucosyl transferase. This suggests that AFN1 may be acting to glucosylate ABA, thereby inactivating it. As the hormone ABA is known to inhibit germination, ABA glucosylation/inactivation could lead to germination in grains expressing AFN1. To test this hypothesis, we have constructed an expression plasmid that encodes …
A Pollen Chase Experiment; Examining Varying Levels Of Embryonic Inbreeding Depression, Emily Wilson, Judy Stone
A Pollen Chase Experiment; Examining Varying Levels Of Embryonic Inbreeding Depression, Emily Wilson, Judy Stone
Undergraduate Research Symposium (UGRS)
A pollen chase experiment was performed upon three Costa Rican populations of Witheringia solanacea to examine the breakdown of genetically enforced self incompatibility (SI) and the extent of embryonic inbreeding depression. Self-pollen was applied in the bud, with outcross pollen applied one day later, and outcross pollinations at both intervals as a control. A variety of responses were found among the populations. BOHS readily accepted self pollen and suffered from very low inbreeding depression. Monteverde and Las Cruces both have lower fruit set with self-pollination precedence indicating that bud pollinations can overcome the self-incompatibility response and that embryonic death due …