Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- ABA (2)
- AFN1 (2)
- ABA glucosyl transferase (1)
- Abscisic acid (1)
- Costa Rica (1)
-
- Embryonic inbreeding depression (1)
- Enzymatic ABA glucosylase activity (1)
- Expression plasmid (1)
- Germination (1)
- Germination stimulation (1)
- MBP AFN1 fusion protein (1)
- Overexpression of AFN1 polypeptide (1)
- PKABA1 (1)
- Plants (1)
- Pollen chase experiment (1)
- Sequence (1)
- Wild oat (1)
- Witheringia solanacea (1)
Articles 1 - 4 of 4
Full-Text Articles in Entire DC Network
Characterization Of Afn1, A Gene Associated With Cereal Grain Germination, Tenzin Tsewang, Russell Johnson
Characterization Of Afn1, A Gene Associated With Cereal Grain Germination, Tenzin Tsewang, Russell Johnson
Undergraduate Research Symposium (UGRS)
The AFN1 gene is transiently expressed in germinating oat grains. As AFN1 is not expressed in dormant oat grains during imbibition, we hypothesize that AFN1 may be involved in stimulating the germination process. Sequence analysis of an AFN1 cDNA clone indicates that the AFN1 polypeptide is similar to a previously identified abscisic acid (ABA) glucosyl transferase. This suggests that AFN1 may be acting to glucosylate ABA, thereby inactivating it. As the hormone ABA is known to inhibit germination, ABA glucosylation/inactivation could lead to germination in grains expressing AFN1. To test this hypothesis, we have constructed an expression plasmid that encodes …
A Pollen Chase Experiment; Examining Varying Levels Of Embryonic Inbreeding Depression, Emily Wilson, Judy Stone
A Pollen Chase Experiment; Examining Varying Levels Of Embryonic Inbreeding Depression, Emily Wilson, Judy Stone
Undergraduate Research Symposium (UGRS)
A pollen chase experiment was performed upon three Costa Rican populations of Witheringia solanacea to examine the breakdown of genetically enforced self incompatibility (SI) and the extent of embryonic inbreeding depression. Self-pollen was applied in the bud, with outcross pollen applied one day later, and outcross pollinations at both intervals as a control. A variety of responses were found among the populations. BOHS readily accepted self pollen and suffered from very low inbreeding depression. Monteverde and Las Cruces both have lower fruit set with self-pollination precedence indicating that bud pollinations can overcome the self-incompatibility response and that embryonic death due …
Overexpression And Purification Of The Oat (Avena Fatua) Protein Afn1 And Construction Of A Pmal/Afn1 Expression Plasmid, Tenzin D. Tsewang
Overexpression And Purification Of The Oat (Avena Fatua) Protein Afn1 And Construction Of A Pmal/Afn1 Expression Plasmid, Tenzin D. Tsewang
Undergraduate Research Symposium (UGRS)
Abscisic acid (ABA) is an important phytohormone with regulatory roles in many physiological processes. ABA expression is induced by environmental stresses such as drought and it is known to be an inhibitor of seed germination. A wild oat (Avena fatua) called AFN1 has been hypothesized to initiate the early stages of germination as its mRNA accumulates in nondormant seed embryos during imbibition. The polypeptide sequence of AFN1 suggests that it is an ABA glucosyl transferase. Glucosylation by AFN1 and thereby inactivation of ABA could lead to seed germination. In order to understand the role of AFN1 in germination, an ample …
Characterization Of The Wheat Grain Pkaba1-Interacting Protein Tawd40, Natalie Wayne
Characterization Of The Wheat Grain Pkaba1-Interacting Protein Tawd40, Natalie Wayne
Undergraduate Research Symposium (UGRS)
Abscisic acid (ABA)-mediated gene expression is a critical component of plant responses to this important hormone, which affects plant growth, development, and responses to environmental stresses. Plant responses to ABA are mediated by a number of factors including PKABA1, an ABA induced protein kinase involved in ABA-suppressed gene expression in cereal grains, and TaWD40, which has previously been shown to physically interact with PKABA1. A full-length 1.9 kb TaWD40 cDNA, CK210682, was sequenced as part of this project. Based on the deduced protein sequence, it is thought that TaWD40 may belong to the family of E3 ubiquitin ligases, possibly targeting …