Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Absorption and Transmission (1)
- Alpha-Synuclein (1)
- Amino Acids (1)
- Aptamers (1)
- BzATP (1)
-
- Charcot-marie-tooth (1)
- Confocal Microscope (1)
- Deoxyribozymes (1)
- Dynein (1)
- Dynein mutation (1)
- Micro experiments (1)
- Neuromuscular disorder (1)
- Nucleic Acids Nitrogen bases (1)
- P2X7 (1)
- Parkinson's Disease (1)
- Proliferation (1)
- Protein Disulfide Isomerase (1)
- Purinergic signaling (1)
- Rab7 (1)
- Satellite glial cells (1)
- Spectroscopy (1)
- Vesicle transport (1)
Articles 1 - 4 of 4
Full-Text Articles in Entire DC Network
Alpha-Synuclein: Insight Into The Hallmark Of Parkinson's Disease As A Target For Quantitative Molecular Diagnostics And Therapeutics, Baggio A. Evangelista
Alpha-Synuclein: Insight Into The Hallmark Of Parkinson's Disease As A Target For Quantitative Molecular Diagnostics And Therapeutics, Baggio A. Evangelista
Honors Undergraduate Theses
Parkinson’s disease (PD) is the second-most common neurodegenerative disease after Alzheimer’s disease. With 500,000 individuals currently living with Parkinson’s and nearly 60,000 new cases diagnosed each year, this disease causes significant financial burden on the healthcare system - amassing to annual expenditures totaling 200 billion dollars; predicted to increase through 2050. The disease phenotype is characterized by a combination of a resting tremor, bradykinesia, muscular rigidity, and depression due to dopaminergic neuronal death in the midbrain. The cause of the neurotoxicity has been largely discussed, with strong evidence suggesting that the protein, alpha-Synuclein, is a key factor. Under native conditions, …
Identifying The Effects Of A Human Dynein Mutation On Gfp-Rab7 Axonal Transport In Embryonic Mouse Neurons, Natalie E. Wilson
Identifying The Effects Of A Human Dynein Mutation On Gfp-Rab7 Axonal Transport In Embryonic Mouse Neurons, Natalie E. Wilson
Honors Undergraduate Theses
The first dynein mutation found in humans that caused disease was a cytoplasmic dynein 1 heavy chain (DYNC1H1 in humans) p.His306Arg mutation, first described by Weedon et al. in 2011. This mutation caused Charcot-Marie-Tooth (CMT) subtype 2O. CMT has a prevalence of approximately 1 in 2500 people, making it the most common hereditary neuromuscular disorder. Cytoplasmic dynein 1 is used by eukaryotic cells for minus-end directed microtubule-based transport of cargo. One such cargo is Rab7, a late endosomal marker. The purpose of this study is to identify the effects of this mutation on the transport of GFP-tagged Rab7 cargo in …
The Response Of Satellite Glial Cells To P2x7 Receptor Activation, Christina D. Kursewicz
The Response Of Satellite Glial Cells To P2x7 Receptor Activation, Christina D. Kursewicz
Honors Undergraduate Theses
Satellite glial cells (SGCs) surround the cell bodies of neurons of the peripheral nervous system, including those of the sensory ganglia. Their close apposition to the neuronal soma allows for bi-directional communication between neurons and SGCs, which are thought to regulate neuronal activity. After nerve injury, SGCs in the dorsal root ganglia contribute to neuropathic pain. Although the mechanisms are not fully understood, SGCs show increased coupling via gap junctions, and communicate with the neuron via bi-directional purinergic signaling after nerve injury. The increased coupling between SGCs and neurons may have implications for chronic pain following peripheral nerve injury. In …
Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera
Micro-Spectroscopy Of Bio-Assemblies At The Single Cell Level, Jeslin Kera
Honors Undergraduate Theses
In this thesis, we investigate biological molecules on a micron scale in the ultraviolet spectral region through the non-destructive confocal absorption microscopy. The setup involves a combination of confocal microscope with a UV light excitation beam to measure the optical absorption spectra with spatial resolution of 1.4 μm in the lateral and 3.6 μm in the axial direction. Confocal absorption microscopy has the benefits of requiring no labels and only low light intensity for excitation while providing a strong signal from the contrast generated by the attenuation of propagating light due to absorption. This enables spatially resolved measurements of single …