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Mid-Ir Biosensor: Detection And Fingerprinting Of Pathogens On Gold Island Functionalized Chalcogenide Films, Chenxu Yu, Ashtosh Ganjoo, H. Jain, C. G. Pantano, Joseph Irudayaraj
Mid-Ir Biosensor: Detection And Fingerprinting Of Pathogens On Gold Island Functionalized Chalcogenide Films, Chenxu Yu, Ashtosh Ganjoo, H. Jain, C. G. Pantano, Joseph Irudayaraj
Chenxu Yu
Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the surface of IR-transparent Ge-containing chalcogenide glass films were formed via thiol chemistry on 20-nm-thick gold islands. As a first step, the protocol was validated by monitoring fluorescently tagged targets to validate binding. FT-IR spectroscopy confirmed that the coating of the films with 20-nm gold did not have a significant effect on the propagation and penetration of IR evanescent waves through the film. The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR fingerprinting. Highly selective detection …
Identification Of Pathogenic Bacteria In Mixed Cultures By Ftir Spectroscopy, Chenxu Yu, Joseph Irudayaraj
Identification Of Pathogenic Bacteria In Mixed Cultures By Ftir Spectroscopy, Chenxu Yu, Joseph Irudayaraj
Chenxu Yu
Routine identification of pathogenic microorganisms predominantly based on nutritional and biochemical tests is a time-consuming process. In this study, a method based on FTIR spectroscopy was developed to detect and identify the presence of five possible pathogenic bacteria in ten different microorganism mixtures with each cocktail containing up to three different species at a concentration of 109 CFU/mL. A mathematical approach based on factoring out the common features in the spectral fingerprints was developed and implemented in conjunction with discriminant analysis. FTIR spectra of the mixtures were directly obtained and analyzed using canonical variate analysis based on the discriminant model …