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Identification Of The Role Of Swra In Copper Induced Poly-Gamma-Glutamic Acid Production In Bacillus Subtilis, Maya Addison
Identification Of The Role Of Swra In Copper Induced Poly-Gamma-Glutamic Acid Production In Bacillus Subtilis, Maya Addison
Scripps Senior Theses
Each year over 2.8 million Americans are infected by an antibiotic resistant strain of bacteria with over 35, 000 dying as a result (CDC). The lack of development of new antibiotics has renewed interest in the antimicrobial properties of copper. Gram positive bacterium such as Bacillus subtilis produce poly-gamma-glutamic acid (γ-PGA) to sequester excess copper ions. swrA has shown to be essential for proper activation of the pgs operon by phosphorylated DegU via an unknown mechanism. This study seeks to determine the role of swrA in copper induced gamma-PGA production and if swrA and ppsB are involved in copper resistance. …
The Influence Of G4 Dna Structures On Stationary Phase Mutagenesis In Bacillus Subtilis, Tatiana Ermi
The Influence Of G4 Dna Structures On Stationary Phase Mutagenesis In Bacillus Subtilis, Tatiana Ermi
UNLV Theses, Dissertations, Professional Papers, and Capstones
Until the late 1980’s it was widely understood that bacterial variance emerges randomly during growth. Research that followed has convincingly shown evidence for mutations arising in non-growing conditions, a phenomenon known as stationary-phase mutagenesis. In Bacillus subtilis, an elegant mutagenic mechanism has been elucidated in non-growing cells that biases mutations to transcribed regions of a subpopulation. One interesting possibility is that mutations can be further biased to hotspots within genes through alternate DNA structures known as non-B DNA. Non-B DNA have been linked to genomic instability and disease in humans, lesser is known about its role in bacteria. Here we …
A Natural Antimicrobial From Bacillus Subtilis As A Biosanitizer For Resilient Membrane Biofilms, Pratishtha Verma
A Natural Antimicrobial From Bacillus Subtilis As A Biosanitizer For Resilient Membrane Biofilms, Pratishtha Verma
Electronic Theses and Dissertations
The microbial attachment and colonization on separation membranes lead to biofilm formation. Some selective isolates within the biofilm constitutive microflora acquire resistance and emerge predominant over prolonged use of the membrane. Thus, proving the cleaning and sanitization protocols to be ineffective in adequately removing resilient biofilms. This subsequently leads to selecting microbial resistance within the constitutive microflora to almost all antimicrobial treatments and hence, creates a need to develop novel alternative strategies to control biofilm formation on membrane surfaces. The first research project under this study was designed to understand the microbial interactions and emergence of predominance within biofilm constitutive …
The Role Of Mfd In Stationary-Phase Oxidative Damage Repair In Bacillus Subtilis, Katelyn E. Porter
The Role Of Mfd In Stationary-Phase Oxidative Damage Repair In Bacillus Subtilis, Katelyn E. Porter
UNLV Theses, Dissertations, Professional Papers, and Capstones
Since the 1950’s it has been shown that bacterial cells accumulate mutations even in non- dividing conditions, but how this type of mutation occurs is still highly debated. In Bacillus subtilis, Mfd, a precursor of the nucleotide excision repair (NER) system, mediates the formation of mutations in stationary-phase or non-replicating cells. In growing cells, Mfd recruits repair when RNA polymerase is stalled during transcription; it then recruits proteins from NER to repair damage. Here, we examine the hypothesis that Mfd mediates the formation of mutations by interacting with cellular components that repair reactive oxygen species (ROS), a natural byproduct of …
Medium Optimization For The Production Of Exopolysaccharide By Bacillus Subtilis Using Synthetic Sources And Agro Wastes, Sirajunnisa Abdul Razack, Vijayagopal Velayutham, Viruthagiri Thangavelu
Medium Optimization For The Production Of Exopolysaccharide By Bacillus Subtilis Using Synthetic Sources And Agro Wastes, Sirajunnisa Abdul Razack, Vijayagopal Velayutham, Viruthagiri Thangavelu
Turkish Journal of Biology
Exopolysaccharides (EPSs) play an extensive role as biopolymers in the environment by replacing synthetic polymers as they are degradable, nontoxic, and produced by microorganisms. An attempt was made to optimize a medium, by the one-factor-at-a-time method, for an enhanced production of EPS from a soil isolate, Bacillus subtilis. The study was carried out by experimenting on various nutrients at different concentrations. EPS was precipitated using ethanol, the total carbohydrate content was determined by phenol sulfuric acid method, and functional groups were detected by Fourier transform infrared (FTIR) spectrophotometry. The finalized medium contained sucrose (20 g/L), yeast extract (5 g/L), NaCl …
Enhancement Of Mycolytic Activity Of An Antagonistic Bacillus Subtilis Through Ethyl Methane Sulfonate (Ems) Mutagenesis, Ashwini Narasimhan, Samantha Suresh, Deepak Bist, Srividya Shivakumar
Enhancement Of Mycolytic Activity Of An Antagonistic Bacillus Subtilis Through Ethyl Methane Sulfonate (Ems) Mutagenesis, Ashwini Narasimhan, Samantha Suresh, Deepak Bist, Srividya Shivakumar
Turkish Journal of Biology
Bacillus subtilis, isolated from the chili rhizosphere, capable of producing 3 mycolytic enzymes (chitinase, \beta-1,3-glucanase, and cellulase), was mutated by the chemical mutagen ethyl methane sulfonate (EMS). Mutants were screened based on their antifungal ability on dual plate assay against Colletotrichum gloeosporioides OGC1, the causal agent of anthracnose disease in fruit crops. EMS mutagenesis yielded 60 isolated mutants on NA plates, of which 3 (M3, M4, and M24) showed loss of antagonism against C. gloeosporioides OGC1 and 6 (M8, M21, M22, M57, M58, and M59) exhibited increased antagonism. The remaining mutants did not show any difference in their antagonistic property. …
Bacteriocin Formation By Dominant Aerobic Sporeformers Isolated From Traditional Maari, Donatien Kabore, Line Thorsen, Dennis Sandris Nielsen, Torben Sune Berner, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jackobsen
Bacteriocin Formation By Dominant Aerobic Sporeformers Isolated From Traditional Maari, Donatien Kabore, Line Thorsen, Dennis Sandris Nielsen, Torben Sune Berner, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Mamoudou H. Dicko Prof., Mogens Jackobsen
Pr. Mamoudou H. DICKO, PhD
The antimicrobial activity of 8 Bacillus spp. and 2 Lysinibacillus spp. representing the predominant aerobic sporeformers during traditional maari fermentations, a traditional fermented baobab seeds product fromBurkina Faso,was investigated. The antimicrobial activitywas assessed against a total of 31 indicator organisms representing various Gram-negative and positive pathogens. The screening showed that 3 Bacillus subtilis strains (B3, B122 and B222) in particular had antimicrobial activity against some Gram-positive organisms and were selected for further studies. Itwas found that the antimicrobial substances producedwere heat stable, in-sensitive to catalase, sensitive to protease and trypsin but resistant to the proteolytic action of papain and proteinase …
Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan
Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan
Turkish Journal of Biology
The effects of various carbon and nitrogen sources on production of \alpha-amylase by Bacillus subtilis and its mutant derivates were investigated. The maximum production of \alpha-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. There was no more significant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract and tryptone were preferred nitrogen sources for amylase production by Bacillus subtilis and mutant U 2-6 strain, respectively. \alpha-Amylases of B. subtilis and its mutant strain (EBUE 5-3) were purified through a series of steps, and characterized. The optimum …