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Preliminary Transcriptome Analysis And Homeobox Gene Isonlation For Tomocerus Minor (Entomobryomorpha: Tomoceridae), Alejandro Alaniz
Preliminary Transcriptome Analysis And Homeobox Gene Isonlation For Tomocerus Minor (Entomobryomorpha: Tomoceridae), Alejandro Alaniz
Theses and Dissertations - UTB/UTPA
Collembola, or “springtails,” are small, edaphic arthropods classified as Entognatha; a group traditionally included as part of Hexapoda and sister-group to the true insects. However, some genetic studies suggest that they may instead have derived from a separate crustacean lineage rather than from a proto-Insecta lineage. The unique abdominal structures that develop on the collembolan ventral abdomen appear to be appendicular in origin – atypical to the canonical, appendage-less insect abdomen ground plan. I developed culture and embryo fixation protocols for; and sequenced and analyzed a multi-stage embryo transcriptome for Tomocerus minor (Collembola: Entomobryomorpha). This analysis annotated 14,417 distinct genes, …
Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino
Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino
Theses and Dissertations - UTB/UTPA
In this study the functional characterization and overexpression of Metal Tolerance Protein (MTP) cDNA were evaluated using a transgenic yeast (Saccharomyces cerevisiae) model system as a preliminary means for the characterization of metal tolerance conferred by putative Group III MTP genes from Arabidopsis thaliana. Metal disc assays for nickel, zinc, cobalt and cadmium treatments were performed to measure the tolerance imparted to yeast by the MTP cDNAs. Statistical analysis from zinc and cadmium assays showed AtMTP1 imparted the highest zinc tolerance in yeast, while AtMTPB1 imparted the highest cadmium and cobalt tolerance. However, AtMTPA2 and AtMTPB produced a decrease in …
Management Of Silverleaf Whitefly, Bemisia Argentifolii Bellows And Perring (Hemiptera: Aleyrodidae), Using Non-Crop Companion Plants In Organically-Managed Cantaloupe Systems In South Texas, Ruth Renee Colyer
Theses and Dissertations - UTB/UTPA
Field trials in three separate phases of research were carried out from March, 2010, to August, 2012, to determine the effects of inter-cropping cantaloupe (Cucumis melo var. inodorus) with select non-crop companion plants in an effort to develop organic production strategies for melons in South Texas without the use of pesticides. A research program was designed and initiated to assess the effects of the companion plants and their ability to: 1) attract insect pollinators, and 2) repel or suppress pest species without affecting beneficial natural enemy complexes. The hypotheses were: 1) that organically-managed cantaloupe systems grown with non-crop companion plants …
Isolation And Characterization Of Polychlorinated Biphenyl (Pcb) Degrading Bacteria Isolated From A Pcb Contaminated Reservoir In Donna, Tx, Sergio Cepeda
Theses and Dissertations - UTB/UTPA
The Donna Irrigation System (DIS) located in Donna, Hidalgo County, TX, was confirmed to be contaminated with polychlorinated biphenyls (PCBs) in 1993 and remediation of the site will be attempted in the future. The need to isolate novel PCB degrading bacteria for bioremediation has been an active area of research in recent decades; thus, it was hypothesized that a variety of microorganisms able to degrade PCBs could be isolated from sediments taken from the DIS. Two genes of the PCB degradation pathway, bphA1 and bphC, were detected in 43 bacterial isolates grown from DIS sediments using molecular and culture-based methodology, …
Rates Of Evolution And Point Mutations Of Bacterial Plant Pathogens Compared To Bacterial Vertebrate Pathogens, Alejandro Cantu
Rates Of Evolution And Point Mutations Of Bacterial Plant Pathogens Compared To Bacterial Vertebrate Pathogens, Alejandro Cantu
Theses and Dissertations - UTB/UTPA
The relationship between a pathogen and its host is a constant interaction; as the host evolves defense mechanisms to protect itself from the pathogen, the pathogen will evolve in order to evade them. During this evolutionary process, the genomes of bacterial pathogens can change due to different selection pressures exerted by the immune system of their respective hosts. This study aimed to determine how differences in selection pressures affect the evolution of plant and vertebrate pathogens. The focus of this study was to analyze population level changes in conserved genes, as well as species level changes in the Type III …