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Biology

UNF Graduate Theses and Dissertations

Theses/Dissertations

2014

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Functional Characterization Of A Putative Disaccharide Membrane Transporter In Crustacean Intestine, Rasheda S. Likely Jan 2014

Functional Characterization Of A Putative Disaccharide Membrane Transporter In Crustacean Intestine, Rasheda S. Likely

UNF Graduate Theses and Dissertations

The mechanisms of transepithelial absorption of dietary sucrose in the American lobster, Homarus americanus, were investigated in this study to determine whether sugars can be transported across an animal gut intact or as monosaccharides following hydrolysis. Lobster intestine was isolated and mounted in a perfusion chamber to characterize the mechanisms of mucosal to serosal (MS) 14C -sucrose transport across the intestine MS fluxes were measured by adding varying concentrations of 14C-sucrose to the perfusate which resulted in a hyperbolic curve following Michaelis-Menten kinetics. The kinetic constants of the proposed sucrose transporter were KM = 15.84 ± 1.81 …


Effect Of Feed Additives On Amino Acid And Dipeptide Transport By Intestines Of American Lobster And Atlantic White Shrimp, Maria Louise Peterson Jan 2014

Effect Of Feed Additives On Amino Acid And Dipeptide Transport By Intestines Of American Lobster And Atlantic White Shrimp, Maria Louise Peterson

UNF Graduate Theses and Dissertations

Previous nutritional physiology research using L-histidine and zinc in American lobster intestine (Homarus americanus) has suggested that these solutes can be co-transported as complexes (Histidine-Zinc-Histidine) across the intestine using a peptide transporter. Furthermore, transport of L-leucine was shown to be inhibited by high calcium concentrations. Dipeptide and bis-complex transport and the role of calcium were investigated in the perfused intestines of lobster and Atlantic white shrimp (Litopenaeus setiferus). Following trans-intestinal transport, serosal medium was analyzed for amino acid composition by gas chromatography. In lobster, the transport of glycylsarcosine (Gly-Sar) from mucosa to serosa was stimulated two-fold …


Transcriptional Regulation Of Neurogenic Atrophy-Induced Gene Expression By Muscle Ring Finger-1 And Myogenic Regulatory Factors, Theodore Olson Jan 2014

Transcriptional Regulation Of Neurogenic Atrophy-Induced Gene Expression By Muscle Ring Finger-1 And Myogenic Regulatory Factors, Theodore Olson

UNF Graduate Theses and Dissertations

Skeletal muscle wasting is a consequence of numerous physiological conditions, including denervation, corticosteroid treatment, immobilization, and aging. The E3 ubiquitin ligases, MuRF1 and MAFbx, are induced under nearly all atrophy conditions and are believed to play a key role in protein degradation in atrophying muscle. However, the preliminary data described in this study provides new evidence that MuRF1 may also act as a transcriptional modulator of atrophy-induced gene activity, including the regulation of MAFbx and MuRF1 expression. To characterize the transcriptional regulation of MuRF1 and MAFbx, reporter gene constructs containing fragments of the proximal promoter regions of these genes were …


The Social Structure And Mating Strategies Of Bottlenose Dolphins (Tursiops Truncatus) In The St. Johns River, Jessica Lea Ermak Jan 2014

The Social Structure And Mating Strategies Of Bottlenose Dolphins (Tursiops Truncatus) In The St. Johns River, Jessica Lea Ermak

UNF Graduate Theses and Dissertations

Across populations, bottlenose dolphins (Tursiops sp.) exhibit a fission-fusion pattern of associations, in which group size and composition change fluidly throughout the day. Beneath this seemingly ephemeral social structure, considerable variation exists across study sites. While females typically have moderate bonds with one another within a large social network, male-male bonds are variable, though males typically take one of two strategies; some males encounter females individually for opportunities to breed while others cooperate within a first-order alliance to collectively herd females. In addition, multi-tiered alliances in which two first-order alliances cooperate to defend or assist in the theft of a …


Reproductive Biology Of The Bonnethead (Sphyrna Tiburo) From The Southeastern U.S. Atlantic Coast, Melissa I. Gonzalez De Acevedo Jan 2014

Reproductive Biology Of The Bonnethead (Sphyrna Tiburo) From The Southeastern U.S. Atlantic Coast, Melissa I. Gonzalez De Acevedo

UNF Graduate Theses and Dissertations

Understanding the life history of marine wildlife is essential for the management of both commercial and recreational fisheries. Bonnetheads (Sphyrna tiburo) are a component of the small coastal shark (SCS) fishery complex, and are caught regularly in both types of fisheries. Despite being well studied in the Gulf of Mexico, little is known about bonnetheads from the U.S. Atlantic coast. The goal of the first component of this study was to provide new, key information on their life history to improve management of U.S. Atlantic populations, particularly by identifying reproductive seasonality, periodicity and fecundity. This was accomplished by examining sexually …


The Effects Of Sampling Design On Abundance And Distribution Of Bottlenose Dolphins In The St. Johns River, Florida, Samantha Ryanne Nekolny Jan 2014

The Effects Of Sampling Design On Abundance And Distribution Of Bottlenose Dolphins In The St. Johns River, Florida, Samantha Ryanne Nekolny

UNF Graduate Theses and Dissertations

The National Oceanic and Atmospheric Administration (NOAA) is tasked with upholding the regulations prescribed in the Marine Mammal Protection Act of 1972. One of the ways this is accomplished is by producing regular stock assessment reports. For these reports, knowledge about a species abundance and distribution is a vital component, allowing informed management decisions to be made that may potentially reduce the natural and anthropogenic impacts on the organisms. In 2010, an Unusual Mortality Event (UME) occurred in the lower St. Johns River, resulting in an unusually large number of bottlenose dolphin strandings. As the data regarding this population was …


The Performance Of Simple Artificial Floating Wetland Communities And Their Effects On Aquatic Nutrient Levels And Algal Abundance, Bradley L. Sleeth Jan 2014

The Performance Of Simple Artificial Floating Wetland Communities And Their Effects On Aquatic Nutrient Levels And Algal Abundance, Bradley L. Sleeth

UNF Graduate Theses and Dissertations

Harmful algal blooms are exponential increases in autotrophic microorganisms that proliferate in such a way that the surrounding environment, the local economy and the health of regional populations are negatively affected. Among the causes of these blooms are anthropogenic inputs of excess nitrogen and phosphorus into the environment through overfertilization. Floating treatment wetlands (FTW) have emerged as a novel method of reducing the negative impacts of these nutrient inputs by using artificial rafts to float normally emergent wetland plants on the surface of water bodies to assimilate excess nutrients. Because their use is so new, only limited research has been …


Effects Of Elevated Salinity And Oxidative Stress On The Physiology Of The Toxigenic Cyanobacterium Microcystis Aeruginosa, Billy Christopher Warhurst Jan 2014

Effects Of Elevated Salinity And Oxidative Stress On The Physiology Of The Toxigenic Cyanobacterium Microcystis Aeruginosa, Billy Christopher Warhurst

UNF Graduate Theses and Dissertations

Harmful algal blooms (HABs) are found worldwide, particularly in places where warm, well-lit, and stagnant waters are common. HABs can have negative effects on aquatic plants and wildlife due to the reduction in light availability associated with turbidity, decrease in O2 availability, and the production of secondary metabolites that can harm or even prove lethal. Aquatic ecosystems are regularly being affected by elevated salinity because of recent water management strategies, episodes of drought, and salt water intrusion. This research focused on how salinity levels ranging from 0-10ppt affected physiological attributes such as cellular growth and abundance, cell mortality, toxin …


Effects Of Asphondylia Borrichiae, Simulated Herbivory, And Nutritional Status On Survival, Flowering, And Seed Viability In Sea Oxeye Daisy (Borrichia Frutescens), Lisa S. Rowan Jan 2014

Effects Of Asphondylia Borrichiae, Simulated Herbivory, And Nutritional Status On Survival, Flowering, And Seed Viability In Sea Oxeye Daisy (Borrichia Frutescens), Lisa S. Rowan

UNF Graduate Theses and Dissertations

Although herbivory and other types of plant damage typically are viewed as detrimental to plant survival and performance, vigorous regrowth, greater seed set, and fitness benefits may be possible when damage to the apical meristem, or actively growing stem terminal, is involved. Such damage releases apical dominance, or the hormonal suppression of lateral buds, activates dormant lateral buds, and enables lateral shoots to grow. Since in plants with terminal flowers, each stem may bear a flower, removal of the apical meristem may result in stem bifurcation and ultimately increase the number of flowers and seeds, thereby increasing potential fitness. In …