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Articles 1 - 15 of 15
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Benthic Macroinvertebrate Pre-Assessment Of A Proposed Restoration In The Grand River, Grand Rapids, Mi, Usa, Matthew L. Bain
Benthic Macroinvertebrate Pre-Assessment Of A Proposed Restoration In The Grand River, Grand Rapids, Mi, Usa, Matthew L. Bain
Masters Theses
River restoration is the dominant field of applied water resources management in the United States. Ecological improvement should be the goal of all river restorations, though many restoration projects fail to produce positive results due to limited scope or inadequate assessment methodologies. Pre-restoration, biotic data is essential for such projects as it can be paired with post-restoration data to gauge ecological outcomes. A major restoration effort is now underway in Michigan's longest river, the Grand River, where it flows through downtown Grand Rapids. The primary restoration measures will occur in-stream at local scale. We conducted a pre- restoration survey for …
Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina
Masters Theses
Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their …
Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin
Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin
Masters Theses
Soil microbiomes play pivotal roles to the health of the environment by maintaining metabolic cycles. One question is how will climate change affect soil bacteria over time and what could the repercussions be. To answer these questions, the Harvard Forest Long-Term Warming Experiment was established to mimic predicted climate change by warming plots of land 5℃ above ambient conditions. In 2017, 14 soil core samples were collected from Barre Woods warming experiment to mark 15 years since the establishment of the soil warming in that location. These samples underwent traditional metatranscriptomics to generate an mRNA library as well as a …
Chemosensory Receptors In Berghia Stephanieae: Bioinformatics And Localization, Kelsi L. Watkins
Chemosensory Receptors In Berghia Stephanieae: Bioinformatics And Localization, Kelsi L. Watkins
Masters Theses
Chemosensation is achieved through the binding of chemical signals to chemoreceptor proteins embedded in the membranes of sensory neurons. The molecular identity of these receptors, as well as the downstream processing of chemosensory signals, has been well studied in arthropods and vertebrates. However, very little is known about molluscan chemosensation. The identity of chemoreceptor proteins in the nudibranch mollusc Berghia stephanieae are unknown. Data from other protostome and molluscan studies suggest Berghia may use ionotropic receptors for some forms of chemoreception. This study used a bioinformatics approach to identify potential chemosensory ionotropic receptors in the transcriptome of Berghia. A …
Influence Of Soil And Land Cover On Chronic Wasting Disease Prevalence In White-Tailed Deer And Mule Deer Across North America, Madison L. Miller
Influence Of Soil And Land Cover On Chronic Wasting Disease Prevalence In White-Tailed Deer And Mule Deer Across North America, Madison L. Miller
Masters Theses
Chronic wasting disease (CWD) is a fatal neurodegenerative disease that infects deer and is caused by a pathogenic prion. CWD is a concerning wildlife disease because it is incurable, potentially poses a risk to human health, and is spreading rapidly. CWD prions are transmitted both directly via bodily fluids and indirectly through environmental reservoirs such as soil. In this study, we investigated the influence of land cover and soil characteristics on CWD prevalence in white-tailed deer and mule deer. We acquired CWD prevalence data from seven North American regions and used ArcGIS to obtain land cover and soil characteristic data …
Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage
Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage
Masters Theses
Clarireedia spp. (formerly Sclerotinia homoeocarpaF.T. Bennett) is the causal agent dollar spot, the most economically important turfgrass disease impacting golf courses in North America. The most effective strategy for dollar spot control is repeated application of multiple classes of fungicides. However, reliance on chemical application has led to resistance to four classes of fungicides as well as multidrug resistance (MDR). Fungi are known to detoxify xenobiotics, like fungicides, through transcriptional regulation of three detoxification phases: modification, conjugation and secretion. Little is known, however, of the protein-protein interactions that facilitate these pathways. Following next-generation RNA sequencing of Clarireedia spp., a …
A Tipping Point In The Ecuadorian Amazon Rainforest: Current And Future Land-Use And Climate Change Trends, Alula Shields
A Tipping Point In The Ecuadorian Amazon Rainforest: Current And Future Land-Use And Climate Change Trends, Alula Shields
Masters Theses
Many regions of the Amazon are experiencing drastic changes as deforestation and climate change drive the world’s largest continuous rainforest towards a ‘tipping point’. These disturbances are changing natural cycles that once past a critical threshold, will mark an unstoppable transition to an altered ecosystem. Losing areas of the Amazon rainforest will have implications for the global climate, global carbon budget, and global hydrological regimes. Scholars have projected these tipping points for areas of the eastern Amazon rainforest, but much less scholarship focuses on the headwaters of the Western Amazon, an area of great cultural and biological importance. Ecuador is …
Geographic Range Size As A Predictor Of Dispersal-Dependent Behavioral Traits In Two Clades Of A Terrestrial Salamander, Teah Evers
Masters Theses
Animal movement has the potential to affect diverse processes within ecology and evolution including range expansion, gene flow, adaptation, and speciation. Two aspects of animal personality that are germane to dispersal are exploratory and aggressive behavior. These behavioral categories may represent a trade-off such that energy invested in territorial defense leaves little energy for movement and dispersal. The Eastern Red-backed Salamander (Plethodon cinereus) is a wide ranging, dispersal limited, terrestrial salamander with well documented phylogeographic divisions. I examined dispersal-relevant behavioral traits within two clades of P. cinereus with disparate geographic ranges. The Northern Clade (NC) has a range extending from …
Alkaloid-Based Chemical Defenses In 11 Species Of Bufonid Poison Frogs From Brazil (Bufonidae: Melanophryniscus), Emily Staufer
Alkaloid-Based Chemical Defenses In 11 Species Of Bufonid Poison Frogs From Brazil (Bufonidae: Melanophryniscus), Emily Staufer
Masters Theses
The term ‘poison frog’ describes members of five anuran families that are capable of sequestering lipophilic, alkaloid-based chemical defenses from their prey. Alkaloid defenses can vary widely within and among poison frog species, mostly linked to differences in availability of arthropod prey at different scales. While research has mainly focused on alkaloid variation within the dendrobatid and mantellid poison frogs, bufonid poison frogs in the genus Melanophryniscus demonstrate high inter- and intra-species alkaloid variation, and some members are capable of both sequestering dietary alkaloids and synthesizing alkaloids metabolically. However, of the 31 species of Melanophryniscus, only eight have ever had …
“Albertania” And “Egbenema”, Panding Biodiversity In The Oculatellaceae (Cyanobacteria)., Mildred Akagha
“Albertania” And “Egbenema”, Panding Biodiversity In The Oculatellaceae (Cyanobacteria)., Mildred Akagha
Masters Theses
To conduct molecular studies of cyanobacteria of Lagos, Nigeria, a total of eight soil/subaerial samples were collected in Lagos State, Nigeria, and used to isolate cyanobacterial cultures that were characterized microscopically, sequenced, and phylogenetically analyzed. Within the resulting set of cultures, a number of Synechococcales were observed, particularly belonging to Oculatellaceae. “Egbenema” and two new putative species of the recently described genus Albertania were found. Both genera belong to a supported clade within the Oculatellaceae that includes Trichotorquatus and Komarkovaea. The two new putative species of Albertania, “A. egbensis” and “A. latericola”, were from …
One Shell Of A Problem: Cumulative Threat Analysis Of Male Sea Turtles Indicates High Anthropogenic Threat For Migratory Individuals And Gulf Of Mexico Residents, Micah Ashford
Masters Theses
Human use of oceans has dramatically increased in the 21st century, with some of the highest rates of change found within the Gulf of Mexico and Caribbean. Sea turtles are particularly vulnerable to anthropogenic stressors in the marine environment as they make lengthy migrations between foraging and breeding sites often along coastal migration corridors. Sea turtles face severe population pressure from humans, yet little is known about how movement and threats interact specifically for male sea turtles. To better understand male sea turtle movement, and the threats they encounter within their expansive ranges, we tagged 40, adult male sea turtles …
Variation In Alkaloid Composition And Sequestration In Dendrobatid Poison Frogs, Katherine R. Waters
Variation In Alkaloid Composition And Sequestration In Dendrobatid Poison Frogs, Katherine R. Waters
Masters Theses
No abstract provided.
Survey Of Diatom Periphyton Assemblages In Lake George, Joy Jackson
Survey Of Diatom Periphyton Assemblages In Lake George, Joy Jackson
Masters Theses
Lake George is an oligotrophic lake that has been monitored by the International Biological Monitoring Program (IBM) since the 1960s. The 51 km long lake is separated into two deep basins, one in the south where the major stream inputs are located, and one in the north where the lake feeds the La Chute River, which empties into Lake Champlain. The two basins are separated by a shallow channel named the “Narrows.” The southern basin has experienced a higher level of development than the northern basin. The aim of this study is to characterize the diatom assemblages in the northern …
Assessment Of Repeatability And Behavioral Syndromes In Genetically Distinct Clades Of Eastern Red-Backed Salamanders (Plethodon Cinereus), Kelsey Garner
Masters Theses
Animal personality implies limited behavioral plasticity and behavioral traits which are correlated across contexts. Correlated behaviors, sometimes referred to as behavioral syndromes, have the potential to inhibit behavioral traits from evolving independently. Limits on behavioral variation can influence a population’s ability to invade new geographic areas and exploit new niches. One way to explore such variation is to examine behavioral syndromes in genetically distinct populations of wide-ranging species that exhibit different dispersal pathways and distances. The personality-dependent dispersal syndrome suggests that certain traits, such as exploratory and risk-taking behavior, are correlated with dispersal success. The Eastern Red-backed Salamander (Plethodon …
Validation Of Edna Metabarcoding : A Comparison To Traditional Survey Methods In Ozark Streams, Veronica Marian Lee
Validation Of Edna Metabarcoding : A Comparison To Traditional Survey Methods In Ozark Streams, Veronica Marian Lee
Masters Theses
Environmental DNA (eDNA) sampling provides a method for assessing fish communities that has potential as a supplement to traditional sampling methods due to its ability to save time as well as its non-invasive nature. This is a method in which from just one sample, eDNA from multiple individual species are able to be sequenced in tandem and the resulting reads identified to describe a community. In order to evaluate this technique and its efficacy for monitoring fish community diversity, we collected water samples alongside surveys performed by the Missouri Department of Conservation sampling program in summers 2020-21. DNA were extracted …