Digital Commons Network^™

Preparing Teaching Assistants To Facilitate Course-Based Undergraduate Research Experiences (Cures) In The Biological Sciences: A Call To Action, Erin Shortlidge, Amie M. Kern, Emma Goodwin, Jeffrey T. Olimpo

Biology Faculty Publications and Presentations

Course-based undergraduate research experiences (CUREs) offer an expanding avenue to engage students in real-world scientific practices. Increasingly, CUREs are instructed by graduate teaching assistants (TAs), yet TAs may be underprepared to facilitate and face unique barriers when teaching CUREs. Consequently, unless TAs are provided professional development (PD) and resources to teach CUREs effectively, they and their students may not reap the assumed benefits of CURE instruction. Here, we describe three perspectives – that of the CURE TA, the CURE designer/facilitator, and the CURE student – that are collectively intended to inform the development of tentative components of CURE TA PD. …

Genetic And Environmental Drivers Of Migratory Behavior In Western Burrowing Owls And Implications For Conservation And Management, Kelly Barr, Christen M. Bossu, Rachel A. Bay, Eric C. Anderson, Jim Belthoff, Lynne A. Trulio, Debra Chromczak, Colleen L. Wisinski, Thomas B. Smith, Kristen C. Ruegg

Biology Faculty Publications and Presentations

Migration is driven by a combination of environmental and genetic factors, but many questions remain about those drivers. Potential interactions between genetic and environmental variants associated with different migratory phenotypes are rarely the focus of study. We pair low coverage whole genome resequencing with a de novo genome assembly to examine population structure, inbreeding, and the environmental factors associated with genetic differentiation between migratory and resident breeding phenotypes in a species of conservation concern, the western burrowing owl (Athene cunicularia hypugaea). Our analyses reveal a dichotomy in gene flow depending on whether the population is resident or migratory, …

Doxorubicin-Induced Modulation Of Tgf-Β Signaling Cascade In Mouse Fibroblasts: Insights Into Cardiotoxicity Mechanisms, Conner Patricelli, Parker Lehmann, Julia Thom Oxford, Xinzhu Pu

Biology Faculty Publications and Presentations

Doxorubicin (DOX)-induced cardiotoxicity has been widely observed, yet the specific impact on cardiac fibroblasts is not fully understood. Additionally, the modulation of the transforming growth factor beta (TGF-β) signaling pathway by DOX remains to be fully elucidated. This study investigated DOX’s ability to modulate the expression of genes and proteins involved in the TGF-β signaling cascade in mouse fibroblasts from two sources by assessing the impact of DOX treatment on TGF-β inducible expression of pivotal genes and proteins within fibroblasts. Mouse embryonic fibroblasts (NIH3T3) and mouse primary cardiac fibroblasts (CFs) were treated with DOX in the presence of TGF-β1 to …

Big Boned: How Fat Storage And Other Adaptations Influenced Large Theropod Foraging Ecology, Cameron Pahl, Luis A. Ruedas

Biology Faculty Publications and Presentations

Dinosaur foraging ecology has been the subject of scientific interest for decades, yet much of what we understand about it remains hypothetical. We wrote an agent-based model (ABM) to simulate meat energy sources present in dinosaur environments, including carcasses of giant sauropods, along with living, huntable prey. Theropod dinosaurs modeled in this environment (specifically allosauroids, and more particularly, Allosaurus Marsh, 1877) were instantiated with heritable traits favorable to either hunting success or scavenging success. If hunter phenotypes were more reproductively successful, their traits were propagated into the population through their offspring, resulting in predator specialists. If selective pressure favored scavenger …

Drivers Of Plankton Community Structure In Intermittent And Continuous Coastal Upwelling Systems–From Microbes And Microscale In-Situ Imaging To Large Scale Patterns, Moritz Schmid, Su Sponaugle, Anne W. Thompson, Kelly T. Sutherland, Robert Cowen

Biology Faculty Publications and Presentations

Eastern Boundary Systems support major fisheries whose early life stages depend on upwelling production. Upwelling can be highly variable at the regional scale, with substantial repercussions for new productivity and microbial loop activity. Studies that integrate the classic trophic web based on new production with the microbial loop are rare due to the range in body forms and sizes of the taxa. Underwater imaging can overcome this limitation, and with machine learning, enables fine resolution studies spanning large spatial scales. We used the In-situ Ichthyoplankton Imaging System (ISIIS) to investigate the drivers of plankton community structure in the northern California …

Germ Cell Recovery, Cryopreservation And Transplantation In The California White Sturgeon, Acipenser Transmontanus, Amie L. Romney, Danielle M. Myers, Fatima R. Martin, Tawney N. Scanlan, Stuart A. Meyers

Biology Faculty Publications and Presentations

The white sturgeon (Acipenser transmontanus) is the largest freshwater fish in North America. Because of the unique life history characteristics of sturgeon, including longevity, late maturation and long spawning intervals, their aquaculture can be a significant investment of resources. As a result of habitat loss and overharvesting, natural populations of white sturgeon are threatened and there is a growing effort to improve conservation aquaculture programs. Germ cell transplantation is an innovative technology previously demonstrated in a variety of fish species to be able to produce a surrogate broodstock. The technique relies upon optimal donor germ cell recovery and transplantation into …

Foxo1 Promotes The Expression Of Canonical Wnt Target Genes In Examined Basal-Like Breast And Glioblastoma Multiforme Cancer Cells, Shania Pintor, Alma Lopez, David Flores, Brianda Lozoya, Bipul Soti, Rishi Pokhrel, Joaquin Negrete, Michael W. Persans, Robert Gilkerson, Bonnie Gunn, Megan Keniry

Biology Faculty Publications and Presentations

Basal-like breast cancer (BBC) and glioblastoma multiforme (GBM) are aggressive cancers associated with poor prognosis. BBC and GBM have stem cell-like gene expression signatures, which are in part driven by forkhead box O (FOXO) transcription factors. To gain further insight into the impact of FOXO1 in BBC, we treated BT549 cells with AS1842856 and performed RNA sequencing. AS1842856 binds to unphosphorylated FOXO1 and inhibits its ability to directly bind to DNA. Gene Set Enrichment Analysis indicated that a set of WNT pathway target genes, including lymphoid enhancer-binding factor 1 (LEF1) and transcription factor 7 (TCF7), were …

Background Acoustics In Terrestrial Ecology, Clinton D. Francis, Jennifer N. Phillips, Jesse R. Barber

Biology Faculty Publications and Presentations

The way in which terrestrial organisms use the acoustic realm is fundamentally important and shapes behavior, populations, and communities, but how background acoustics, or noise, influence the patterns and processes in ecology is still relatively understudied. In this review, we summarize how background acoustics have traditionally been studied from the signaling perspective, discuss what is known from a receiver's perspective, and explore what is known about population- and community-level responses to noise. We suggest that there are major gaps linking animal physiology and behavior in noise to fitness; that there is a limited understanding of variation in hearing within and …

Biocrusts Indicators Of Livestock Grazing Effects On Soil Stability In Sagebrush Steppe: A Case Study From A Long-Term Experiment In The Northern Great Basin, Stella M. Copeland, Lea A. Condon, Roger Rosentreter, Jesse E. D. Miller, Maya Kahn-Abrams

Biology Faculty Publications and Presentations

Biocrusts are sensitive to changes in livestock grazing intensity in arid rangelands and may be useful indicators of ecosystem functions, particularly soil properties like soil stability, which may suggest the potential for soil erosion. We compared biocrust community composition and surface soil stability in a big sagebrush (Artemisia tridentata) steppe rangeland in the northwestern Great Basin in several paired sites, with or without long-term cattle grazing exclusion, and similar soils (mostly sandy loams), climate, and vegetation composition. We found that livestock grazing was associated with both lower surface soil stability and cover of several biocrust morphogroups, especially lichens, compared with …

Sex And Stress Modulate Pupal Defense Response In Tobacco Hornworm, Sukhman Singh, Sakshi Watts, Ishveen Kaur, Isabella Rodriguez, Jessica Ayala, Dariela Rodriguez, Elian Martin, Rupesh R. Kariyat

Biology Faculty Publications and Presentations

In insects, larval and adult defenses against predators have been well studied. However, pupal (also known as resting stage) defenses have been overlooked and not examined thoroughly. Although some pupa possess antipredator strategies such as hairs, spines, cryptic coloration, and exudation of chemicals, few studies have tested these responses and the factors affecting them. Here, we investigated the behavioral responses in tobacco hornworm Manduca sexta that pupates in soil by introducing an external stimulus using vibrations from an electric toothbrush to mimic predation. We observed that M. sexta made violent wriggling (twitching), followed by pulsating movements in response to the …

Computational Analysis Of Plasma Lipidomics From Mice Fed Standard Chow And Ketogenic Diet, Amy L. Seufert, James W. Hickman, Jaewoo Choi, Brooke A. Napier

Biology Faculty Publications and Presentations

Dietary saturated fatty acids (SFAs) are upregulated in the blood circulation following digestion. A variety of circulating lipid species have been implicated in metabolic and inflammatory diseases; however, due to the extreme variability in serum or plasma lipid concentrations found in human studies, established reference ranges are still lacking, in addition to lipid specificity and diagnostic biomarkers. Mass spectrometry is widely used for identification of lipid species in the plasma, and there are many differences in sample extraction methods within the literature. We used ultra-high performance liquid chromatography (UPLC) coupled to a high-resolution hybrid triple quadrupole-time-of-flight (QToF) mass spectrometry (MS) …

Diverse And Variable Community Structure Of Picophytoplankton Across The Laurentian Great Lakes, John Gale, Carey P. Sweeney, Sarah Paver, Maureen L. Coleman, Anne W. Thompson

Biology Faculty Publications and Presentations

The Laurentian Great Lakes provide economic support to millions of people, drive biogeochemical cycling, and are an important natural laboratory for characterizing the fundamental components of aquatic ecosystems. Small phytoplankton are important contributors to the food web in much of the Laurentian Great Lakes. Here, for the first time, we reveal and quantify eight phenotypically distinct picophytoplankton populations across the Lakes using a multilaser flow cytometry approach, which distinguishes cells based on their pigment phenotype. The distributions and diversity of picophytoplankton flow populations varied across lakes and depths, with Lake Erie standing out with the highest diversity. By sequencing sorted …

Dna Barcoding Of Springsnails (Mollusca, Gastropoda, Caenogastropoda) Endemic To The Trans-Pecos Region Of Texas (Usa), Kathryn E. Perez, Vanessa Saenz, Natalia Salazar-Lozano, Benjamin F. Schwartz, Benjamin T. Hutchins

Biology Faculty Publications and Presentations

In desert environments, unique communities depend on groundwater from springs, including a diverse radiation of small (<5 >mm) snails found in the desert across the southwestern United States. Nearly all springsnail species are considered critically imperiled with their existence depending on maintenance of spring-flows in regions of declining water availability. Extant, endemic springsnails in the Trans-Pecos region of Texas include one species of Pseudotryonia Hershler, 2001, five nominal Tryonia W. Stimpson, 1865 (Cochliopidae) and seven Pyrgulopsis Call & Pilsbry, 1886 (Hydrobiidae). Four of these are classified as endangered under the US Endangered Species Act. Surveys for springsnails were conducted at …

Illegal Shooting Is Now A Leading Cause Of Death Of Birds Along Power Lines In The Western Usa, Eve C. Thomason, Natalie J. S. Turley, James R. Belthoff, Tara J. Conkling, Todd E. Katzner

Biology Faculty Publications and Presentations

Human actions, both legal and illegal, affect wildlife in many ways. Inaccurate diagnosis of cause of death undermines law enforcement, management, threat assessment, and mitigation. We found 410 dead birds collected along 196 km of power lines in four western USA states during 2019–2022. We necropsied these carcasses to test conventional wisdom suggesting that electrocution is the leading cause of death of birds at electrical infrastructure. Of 175 birds with a known cause of death, 66% died from gunshot. Both raptors and corvids were more likely to die from gunshot than from other causes, along both transmission and distribution lines. …

Cullin 3–Mediated Regulation Of Intracellular Iron Homeostasis Promotes Thymic Invariant Nkt Cell Maturation, Emily L. Yarosz, Ajay Kumar, Jeffrey Singer, Cheong-Hee Chang

Biology Faculty Publications and Presentations

The E3 ubiquitin ligase cullin 3 (Cul3) is critical for invariant NKT (iNKT) cell development, as iNKT cells lacking Cul3 accumulate in the immature developmental stages. However, the mechanisms by which Cul3 mediates iNKT cell development remain unknown. In this study, we investigated the role of Cul3 in both immature and mature thymic iNKT cells using a mouse model with a T cell–specific deletion of Cul3. We found that mature iNKT cells lacking Cul3 proliferated and died more than wild-type cells did. These cells also displayed increased glucose metabolism and autophagy. Interestingly, we found that tight regulation of iron homeostasis …

A Genotype × Environment Experiment Reveals Contrasting Response Strategies To Drought Between Populations Of A Keystone Species (Artemisia Tridentata; Asteraceae), Anthony E. Melton, Kara Moran, Peggy Martinez, Paige Ellestad, Erin Milliken, Walker Morales, Andrew W. Child, Bryce A. Richardson, Marcelo Serpe, Stephen J. Novak, Sven Buerki

Biology Faculty Publications and Presentations

Western North America has been experiencing persistent drought exacerbated by climate change for over two decades. This extreme climate event is a clear threat to native plant communities. Artemisia tridentata is a keystone shrub species in western North America and is threatened by climate change, urbanization, and wildfire. A drought Genotype × Environment (G × E) experiment was conducted to assess phenotypic plasticity and differential gene expression in A. tridentata. The G × E experiment was performed on diploid A. tridentata seedlings from two populations (one from Idaho, USA and one from Utah, USA), which experience differing levels of …

How Enhancers Regulate Wavelike Gene Expression Patterns, Christine Mau, Heike Rudolf, Frederic Strobl, Benjamin Schmid, Timo Regensburger, Ralf Palmisano, Ernst Hk Stelzer, Leila Taher, Ezzat El-Sherif

Biology Faculty Publications and Presentations

A key problem in development is to understand how genes turn on or off at the right place and right time during embryogenesis. Such decisions are made by non-coding sequences called ‘enhancers.’ Much of our models of how enhancers work rely on the assumption that genes are activated de novo as stable domains across embryonic tissues. Such a view has been strengthened by the intensive landmark studies of the early patterning of the anterior-posterior (AP) axis of the Drosophila embryo, where indeed gene expression domains seem to arise more or less stably. However, careful analysis of gene expression patterns in …

Embryonic Development Of Natural Annual Killifish Populations Of The Genus Austrolebias: Evolutionary Parallelism And The Role Of Environment, Hellen Schlueb, Daniel Blanco, Daniel García, Jason Podrabsky

Biology Faculty Publications and Presentations

1. Repeated, independent emergence of the same trait within different phylogenetic lineages is termed parallel evolution. It typically occurs as a result of similar selective pressures. Annual killifish have adapted to survive in the extreme habitat of temporary pools on three continents and present an especially amenable system for studying fundamental principles of evolutionary parallelism. When the pools dry, annual killifish embryos survive through the dry phase in the bottom substrate in a stage of dormancy—a diapause. The diapause is a complex set of three different developmental stages, none of which is obligate, thus leading to a multitude of potential developmental …

Effects Of Heterozygous Deletion Of Autism-Related Gene Cullin-3 In Mice, Qiang-Qiang Xia, Angela K. Walker, Chenghu Song, Jing Wang, James A. Mobley, Jeffrey Singer, Anju Singh, Craig M. Powell

Biology Faculty Publications and Presentations

Autism Spectrum Disorder (ASD) is a developmental disorder in which children display repetitive behavior, restricted range of interests, and atypical social interaction and communication. CUL3, coding for a Cullin family scaffold protein mediating assembly of ubiquitin ligase complexes through BTB domain substrate-recruiting adaptors, has been identified as a high-risk gene for autism. Although complete knockout of Cul3 results in embryonic lethality, Cul3 heterozygous mice have reduced CUL3 protein, demonstrate comparable body weight, and display minimal behavioral differences including decreased spatial object recognition memory. In measures of reciprocal social interaction, Cul3 heterozygous mice behaved similarly to their wild-type littermates. In …

Evolutionary Codependency: Insights Into The Mitonuclear Interaction Landscape From Experimental And Wild Caenorhabditis Nematodes, Suzanne Estes, Zachary P. Dietz, Vaishali Katju, Ulfar Bergthorsson

Biology Faculty Publications and Presentations

Aided by new technologies, the upsurgence of research into mitochondrial genome biology during the past 15 years suggests that we have misunderstood, and perhaps dramatically underestimated, the ongoing biological and evolutionary significance of our long-time symbiotic partner. While we have begun to scratch the surface of several topics, many questions regarding the nature of mutation and selection in the mitochondrial genome, and the nature of its relationship to the nuclear genome, remain unanswered. Although best known for their contributions to studies of developmental and aging biology, Caenorhabditis nematodes are increasingly recognized as excellent model systems to advance understanding in these …

Biological Flora Of Coastal Freshwater And Brackish Marshes: Cladium Jamaicense Crantz, Richard Stalter, Robert I. Lonard

Biology Faculty Publications and Presentations

Cladium jamaicense Crantz, also known as sawgrass, has a broad distributional range from the Atlantic coast of Virginia to Florida, to southern Texas, the Caribbean, and along the Atlantic coast of Mexico to Central America, and to Brazil. Cladium jamaicense typically occurs in oligotrophic sloughs and fresh and brackish marshes where optimal salinity values range from 0 to 3.5 ppt. This species is a long-lived perennial with a highly developed rhizome system with rhizomes up to 20 cm long and 2.5 to 10 mm in diameter. Asexual reproduction is common. Its fibrous root system comprises short dauciform roots characterized by …

Novel Viruses Of The Family Partitiviridae Discovered In Saccharomyces Cerevisiae, Angela M. Crabtree, Ignacio De La Higuera, Kenneth Stedman, Multiple Additional Authors

Biology Faculty Publications and Presentations

It has been 49 years since the last discovery of a new virus family in the model yeast Saccharomyces cerevisiae. A large-scale screen to determine the diversity of double-stranded RNA (dsRNA) viruses in S. cerevisiae has identified multiple novel viruses from the family Partitiviridae that have been previously shown to infect plants, fungi, protozoans, and insects. Most S. cerevisiae partitiviruses (ScPVs) are associated with strains of yeasts isolated from coffee and cacao beans. The presence of partitiviruses was confirmed by sequencing the viral dsRNAs and purifying and visualizing isometric, non-enveloped viral particles. ScPVs have a typical bipartite …

Predation Of Lepidophyma Flavimaculatum (Squamata: Xantusiidae) By Basiliscus Plumifrons (Squamata: Corytophanidae), Mauricio Quirós Rosales, José Manuel Mora, Randy Alvarado

Biology Faculty Publications and Presentations

Lizards are the most diversified group of living reptiles with 7176 species worldwide (Uetz 2022). They play key roles in ecosystems where they are prey to a wide variety of predators, including snakes, other lizards, mammals, birds, and invertebrates (Pianka and Vitt 2006). The tropical forests of the new world contain a high variety of species of different sizes, shapes, and colors. In Costa Rica in particular, at least 80 species of lizards have been reported (Leenders 2019). They occupy highly diverse habitat types where they participate in food webs that involve many other species of vertebrates and invertebrates.

Habitat Use And Winter Site Fidelity Of Lincoln’S Sparrow (Melospiza Lincolnii) In The Lower Rio Grande Valley Of Texas, Timothy Brush, Hugh E. Conway, Catherine C. Brush

Biology Faculty Publications and Presentations

No abstract provided.

American Kestrel Migration: Insights And Challenges From Tracking Individuals Across The Annual Cycle, Anjolene R. Hunt, Jesse L. Watson, Jason M. Winiarski, Ron R. Porter, Julie A. Heath

Biology Faculty Publications and Presentations

Natural variation in migratory strategies across the range of the American Kestrel (Falco sparverius) creates a unique opportunity for comparative research of annual cycles. However, it can be logistically and technically challenging to track such a small but highly mobile species. We tagged American Kestrels with light-level geolocators or satellite transmitters with the aim of estimating migration timing and connectivity, and we monitored a subset of satellite-tagged individuals during the breeding season to assess transmitter function and wear. We recovered geolocators from six of 49 (12%) tagged individuals. One geolocator-tagged individual migrated approximately 1235 km from its Idaho …

Pathogenicity Of Multiple Providencia Species (Enterobacteriales: Morganellaceae) To The Mass-Reared Mexican Fruit Fly (Diptera: Tephritidae), Bacilio Salas, Hugh E. Conway, Don C. Vacek, Christopher Vitek, Erin Schuenzel

Biology Faculty Publications and Presentations

Mexican fruit fly (Anastrepha ludens (Loew)) (Diptera: Tephritidae) represents a major threat to fruit production in the Western Hemisphere. Sterile insect technique is used to suppress and eradicate wild populations. Success of this control method necessitates weekly production of hundreds of millions of flies, their sterilization by irradiation, and their aerial release. Diet needed to produce large fly numbers are conducive to the spread of bacteria. Pathogenic bacteria were isolated from 3 rearing facilities and from multiple sources: eggs, larvae, pupae and spent diet, and were found to include some isolates identified to the genus Providencia (Enterobacteriales: Morganellaceae). We …

A New Frontier For Fat: Dietary Palmitic Acid Induces Innate Immune Memory, Amy L. Seufert, Brooke A. Napier

Biology Faculty Publications and Presentations

Dietary saturated fats have recently been appreciated for their ability to modify innate immune cell function, including monocytes, macrophages, and neutrophils. Many dietary saturated fatty acids (SFAs) embark on a unique pathway through the lymphatics following digestion, and this makes them intriguing candidates for inflammatory regulation during homeostasis and disease. Specifically, palmitic acid (PA) and diets enriched in PA have recently been implicated in driving innate immune memory in mice. PA has been shown to induce long-lasting hyper-inflammatory capacity against secondary microbial stimuli in vitro and in vivo, and PA-enriched diets alter the developmental trajectory of stem cell progenitors in …

A Field‐Capable Rapid Plant Dna Extraction Protocol Using Microneedle Patches For Botanical Surveying And Monitoring, Jonathan Selz, Nicolas R. Adam, Céline E. M. Magrini, Fulvia Malvido Montandon, Sven Buerki, Sebastian J. Maerkl

Biology Faculty Publications and Presentations

Premise: A novel protocol for rapid plant DNA extraction using microneedles is proposed, which supports botanic surveys, taxonomy, and systematics. This protocol can be conducted in the field with limited laboratory skills and equipment. The protocol is validated by sequencing and comparing the results with QIAGEN spin-column DNA extractions using BLAST analyses.

Methods and Results: Two sets of DNA extractions were conducted on 13 species spanning various leaf anatomies and phylogenetic lineages: (i) fresh leaves were punched with custom polymeric microneedle patches to recover genomic DNA, or (ii) QIAGEN DNA extractions. Three plastid (matK, rbcL, and trnH-psbA …

Root Traits Of Perennial C4 Grasses Contribute To Cultivar Variations In Soil Chemistry And Species Patterns In Particulate And Mineral-Associated Carbon Pool Formation, Megan J. Kelly-Slatten, Catherine E. Stewart, Malak M. Tfaily, Julie D. Jastrow, Abigail Sasso, Marie-Anne De Graaff

Biology Faculty Publications and Presentations

Recent studies have indicated that the C₄ perennial bioenergy crops switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii) accumulate significant amounts of soil carbon (C) owing to their extensive root systems. Soil C accumulation is likely driven by inter- and intraspecific variability in plant traits, but the mechanisms that underpin this variability remain unresolved. In this study we evaluated how inter- and intraspecific variation in root traits of cultivars from switchgrass (Cave-in-Rock, Kanlow, Southlow) and big bluestem (Bonanza, Southlow, Suther) affected the associations of soil C accumulation across soil fractions using stable isotope techniques. Our …

Microbial Associations With Metcalf’S Tryonia, Tryonia Metcalfi (Gastropoda: Cochliopidae), An Imperiled Ci´Enega Endemic, Russell L. Minton, Kathryn E. Perez

Biology Faculty Publications and Presentations

The Chihuahuan Desert swamps are a hotspot for imperiled organisms including freshwater springsnails and bacteria. Many of these taxa are endemic to the desert and to the individual waterbodies where they occur. Efforts to conserve diversity in these threatened areas must account for the life history of the organisms, but also interactions between organisms including microbes. We documented the microbial assemblage associated with Tryonia metcalfi, a critically imperiled freshwater snail endemic to a ciénega system in western Texas. We identified 14 bacterial families in our snail samples and determined a core assemblage of 19 bacterial taxa (4 of which …