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Daughter Cells Of Saccharomyces Cerevisiae From Old Mothers Display A Reduced Life Span, Nicanor Austriaco, Brian K. Kennedy, Leonard Guarente Dec 1994

Daughter Cells Of Saccharomyces Cerevisiae From Old Mothers Display A Reduced Life Span, Nicanor Austriaco, Brian K. Kennedy, Leonard Guarente

Biology Faculty Publications

The yeast Saccharomyces cerevisiae typically divides asymmetrically to give a large mother cell and a smaller daughter cell. As mother cells become old, they enlarge and produce daughter cells that are larger than daughters derived from young mother cells. We found that occasional daughter cells were indistinguishable in size from their mothers, giving rise to a symmetric division. The frequency of symmetric divisions became greater as mother cells aged and reached a maximum occurrence of 30% in mothers undergoing their last cell division. Symmetric divisions occurred similarly in rad9 and ste12 mutants. Strikingly, daughters from old mothers, whether they arose …


Second Intermediate Host-Specificity Of Haematoloechus Complexus And Haematoloechus Medioplexus (Digenea: Haematoloechidae), Scott D. Snyder, John J. Janovy Dec 1994

Second Intermediate Host-Specificity Of Haematoloechus Complexus And Haematoloechus Medioplexus (Digenea: Haematoloechidae), Scott D. Snyder, John J. Janovy

Biology Faculty Publications

Second intermediate host-specificity was examined for two species of the frog lung fluke genus Haematoloechus. Nine species of freshwater arthropods were exposed to cercariae of H. complexus and H. medioplexus. Metacercariae of H. complexus developed in all arthropod species used. Metacercariae of H. medioplexus developed only in anisopteran odonate naiads. This difference in host utilization may have epizootiological implications. The potential development of H. complexus in a greater number of arthropods than H. medioplexus may increase the chances of ingestion of H. complexus by an anuran host. The range of arthropods parasitized by H. complexus indicates that host-specificity …


Phylogenetic Analysis Of Segment 10 From African Horsesickness Virus And Cognate Genes From Other Orbiviruses, Rafael O. De Sá, Marla Zellner, Marvin J. Grubman Mar 1994

Phylogenetic Analysis Of Segment 10 From African Horsesickness Virus And Cognate Genes From Other Orbiviruses, Rafael O. De Sá, Marla Zellner, Marvin J. Grubman

Biology Faculty Publications

Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1,4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences were analyzed to assess evolutionary relationships among serotypes using cladistics. Based on this analysis the data support a close relationship between serotypes 4 and 9 and between serotypes 1 and 8 and a closer relationship of serotype 3 to the 4 and 9 group.


Development And Application Of A Thermistor Current Meter, Carl M. Way, Albert J. Burky, Christine Miller-Way Jan 1994

Development And Application Of A Thermistor Current Meter, Carl M. Way, Albert J. Burky, Christine Miller-Way

Biology Faculty Publications

This report provides details for the construction of a hot-bead thermistor current meter which is capable of measuring water velocities on a millimeter spatial scale and for the construction of a compact and accurate calibration system. Hot-bead thermistor current meters can be built with response times of 200 ms capable of measuring velocities between 0.1 and 80 cm s-1. The construction of a sturdy probe for application in lotic systems such as high gradient Hawaiian streams was achieved by the use of heavy-duty acrylic tubing, small stainless steel gas-chromatography tubing, and flexible Tygon spaghetti tubing. An acrylic handle anchors the …


Considerations For Evaluating Ultraviolet Radiation-Induced Genetic Damage Relative To Antarctic Ozone Depletion, Deneb Karentz Jan 1994

Considerations For Evaluating Ultraviolet Radiation-Induced Genetic Damage Relative To Antarctic Ozone Depletion, Deneb Karentz

Biology Faculty Publications

Springtime ozone depletion over the Antarctic results in increased UVB in local marine environments. It has been established that decreases in primary productivity occur with decreases in ozone concentrations, but the impact of increased UVB on the functioning and stability of the ecosystem has not yet been determined. Very little has been done to evaluate the potential for genetic damage caused by the increase in UVB, and this type of damage is most significant relative to the fitness and maintenance of populations. An essential problem in evaluating genotoxic effects is the lack of appropriate techniques to sample and quantify genetic …


Energy Conservation By Formation Swimming - Metabolic Evidence From Ducklings, Frank E. Fish Jan 1994

Energy Conservation By Formation Swimming - Metabolic Evidence From Ducklings, Frank E. Fish

Biology Faculty Publications

No abstract provided.


Nectar-Seeking Visits By Butterflies In A Tallgrass Prairie Remnant In Eastern Nebraska, Tanya Bray Jan 1994

Nectar-Seeking Visits By Butterflies In A Tallgrass Prairie Remnant In Eastern Nebraska, Tanya Bray

Biology Faculty Publications

Stolley Prairie, a tallgrass virgin prairie remnant in eastern Nebraska, was visited 20 times between May 25 and August 14, 1988. Fifty-two species offorbs were observed in bloom during this period. Twenty-seven species of butterflies were observed and 21 species made 262 nectar-seeking visits to 21 plant species. While numbers offorbs in bloom did not strongly correlate with numbers of butterflies present, peaks of butterfly occurrence appeared to follow peaks of blossom abundance. The number of plant species utilized by a species of butterfly ranged from one to nine. Plants with an abundance of nectar such as common milkweed and …


What Are Squirrels Hiding, Michael Steele, Peter D. Smallwood Jan 1994

What Are Squirrels Hiding, Michael Steele, Peter D. Smallwood

Biology Faculty Publications

The half-eaten acorns of squirrels are clues to their complex relationship with oaks. The special relationship between squirrels and acorns is examined.


Terrapene Carolina Carolina, Joseph C. Mitchell, Rafael O. De Sá Jan 1994

Terrapene Carolina Carolina, Joseph C. Mitchell, Rafael O. De Sá

Biology Faculty Publications

TERRAPENE CAROLINA CAROLINA (Eastern Box Turtle). REPRODUCTION. Female Terrapene carolina containing oviductal eggs have been recorded in Virginia from 26 May to 25 July (Mitchell, in press. The Reptiles of Virginia. Smithsonian Inst. Press, Washington, D.C.). Other reports of reproduction in Terrapene (see list in Ernst and McBreen 1991. Cat. Amer. Amphib. Rept. 512.1-512.13) indicate that nesting occurs in late spring and early summer throughout its range.