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The Dictyostelium Discoideum Genome Lacks Significant Dna Methylation And Uncovers Palindromic Sequences As A Source Of False Positives In Bisulfite Sequencing, Robert Drewell, Tayla C. Cormier, Jacob L. Steenwyk, James St Denis, Javier F. Tabima, Jacqueline Dresch, Denis A. Larochelle
The Dictyostelium Discoideum Genome Lacks Significant Dna Methylation And Uncovers Palindromic Sequences As A Source Of False Positives In Bisulfite Sequencing, Robert Drewell, Tayla C. Cormier, Jacob L. Steenwyk, James St Denis, Javier F. Tabima, Jacqueline Dresch, Denis A. Larochelle
Biology
DNA methylation, the addition of a methyl (CH3) group to a cytosine residue, is an evolutionarily conserved epigenetic mark involved in a number of different biological functions in eukaryotes, including transcriptional regulation, chromatin structural organization, cellular differentiation and development. In the social amoeba Dictyostelium, previous studies have shown the existence of a DNA methyltransferase (DNMA) belonging to the DNMT2 family, but the extent and function of 5-methylcytosine in the genome are unclear. Here, we present the whole genome DNA methylation profile of Dictyostelium discoideum using deep coverage replicate sequencing of bisulfite-converted gDNA extracted from post-starvation cells. We find an overall …