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Species Level Differences In The Ecology Of Two Neotropical Tadpole Species: Responses To Nonlethal Predators And The Roles Of Competition And Resource Use, Zacharia Costa Dec 2011

Species Level Differences In The Ecology Of Two Neotropical Tadpole Species: Responses To Nonlethal Predators And The Roles Of Competition And Resource Use, Zacharia Costa

Theses and Dissertations

Closely related species at the same trophic level are often considered to be ecologically equivalent. However, it is clear that individuals species can have unique functional roles that drive community and ecosystem processes. In this study we examine the growth responses of two Neotropical hylid tadpole species, Agalychnis callidryas and Dendropsophus ebraccatus, to intraspecific and interspecific competition. We also look at density-dependent effects of each on phytoplankton, periphyton and zooplankton, as well as their responses to a caged dragonfly predator through ontogeny. Intraspecific competition affected both species similarly, and their effects on resources were qualitatively similar but quantitatively different. Predators …


Evaluation Of Brook Trout (Salvelinus Fontinalis) Introductions And Re-Introductions Into Four Virginia Blue Ridge Mountain Streams, Michael Isel Iii Dec 2011

Evaluation Of Brook Trout (Salvelinus Fontinalis) Introductions And Re-Introductions Into Four Virginia Blue Ridge Mountain Streams, Michael Isel Iii

Theses and Dissertations

Approximately 100 brook trout were transplanted into each of four Virginia streams in September 2008. Garth Run and Kinsey Run were re-introductions, and Wildcat Hollow and Sweet Run were new introductions. Single pass electrofishing (EF) surveys were conducted with a backpack electrofisher five times during the study. Selected trout > 70 mm but < 100 mm and all trout > 100 mm received a Biomark® 134.2 kHz passive integrated transponder (PIT) tag to identify individual fish upon recapture. Trout were present in all streams almost three years post introductions. Catches decreased across the first four surveys. Young of year catch rates severely decreased from June 2009 (n = …


Factors Controlling Dissolved Organic Carbon Lability And Ecological Fate In The East Branch Swift River, Massachusettes, Hall Eric Dec 2011

Factors Controlling Dissolved Organic Carbon Lability And Ecological Fate In The East Branch Swift River, Massachusettes, Hall Eric

Theses and Dissertations

Fluvial systems have been estimated to transform, transport, or store 2.75 petagrams (Pg) of Organic Carbon (OC) per year. Although approximately 1Pg per year of terrestrial carbon is fluxed to the atmosphere through inland waters, little is known about the factors regulating its eventual ecological fate. 28 day lability incubations were conducted concurrent with the measurement of several environmental parameters including discharge, nutrient concentration, DO13C, and DOC:DON at several sites along Bigelow Brook and the East Branch of the Swift River, Massachusetts. Temporal and spatial variation of DOC, DOC:DON and DO13C were explored. Two distinct DOC consumption rates, short and …


Shrubs As Sentinels Of Ordnance Contamination: Using Plant Physiology And Remote Sensing To Detect Tnt In Soils, Kathryn Rubis Nov 2011

Shrubs As Sentinels Of Ordnance Contamination: Using Plant Physiology And Remote Sensing To Detect Tnt In Soils, Kathryn Rubis

Theses and Dissertations

Methods for rapid, safe and effective detection of unmapped buried ordnance are vital to the protection of humans and environmental quality throughout the world. This study aimed to investigate the use of phytosensing and to understand the physiological response of woody plants to 2,4,6-trinitrotoluene (TNT) contamination. Baccharis halimifolia were potted in soils containing various concentrations of TNT and physiological responses were observed over a 9-week experimental period. Measurements included the collection of remotely sensed data, such as hyperspectral reflectance and chlorophyll fluorescence, and traditional plant-level physiological data. In accordance with the hypothesis, low levels of TNT improved physiological response in …


Composition Of Suspended And Benthic Particulate Matter In The Tidal Freshwater James River, Anne Schlegel Sep 2011

Composition Of Suspended And Benthic Particulate Matter In The Tidal Freshwater James River, Anne Schlegel

Theses and Dissertations

Investigating linkages between the compositions of suspended (seston) and benthic particulate matter is important to the understanding of organic matter (OM) cycling and nutrient retention in aquatic systems. We compared the quantity and quality of the truly suspended (TS) and settleable (SB) fractions of seston as well as benthic particulate matter in the tidal freshwater James River, Virginia. The mass of seston and OM was consistently higher in the TS fraction compared to the SB fraction. OM was preferentially retained in the TS fraction relative to seston. The proportional contribution of OM constituents (chlorophyll a, particulate organic carbon and nitrogen) …


The Abundance And Infection Status Of Anopheles Spp. Mosquitoes At Three Sites In Loudoun County, Northern Virginia, Priya Krishnan Aug 2011

The Abundance And Infection Status Of Anopheles Spp. Mosquitoes At Three Sites In Loudoun County, Northern Virginia, Priya Krishnan

Theses and Dissertations

Malaria is a re-emerging infectious disease with approximately half of the world's population at risk. In the US, since the 1950’s the Center for Disease Control (CDC) has been reporting between 1,000 and 1,500 cases of malaria every year. A majority of these cases were among US travellers and were attributed to Plasmodium falciparum. In August 2002, two cases of human malaria due to Plasmodium vivax were reported in Loudoun County, Northern Virginia. The Center for Disease Control and Prevention concluded that these cases were acquired locally. This was because of an absence of other risk factors such as international …


Legacy Sediments In Streams - Effects On Nutrient Partitioning During Simulated Re-Suspension Events., Molly Sobotka Aug 2011

Legacy Sediments In Streams - Effects On Nutrient Partitioning During Simulated Re-Suspension Events., Molly Sobotka

Theses and Dissertations

Anthropogenic inputs of nutrients and sediment are a widespread problem in U.S. streams causing localized impairment and contributing to eutrophication of coastal habitats. Sediments and dissolved nutrients interact through diverse processes including ion exchange, sorption and biotic assimilation by particle-bound bacteria. This study examined the effects of sediment re-suspension on nutrient partitioning in lab microcosms using fine benthic matter collected from two Virginia Coastal Plain streams. Kimages Creek was recently restored following dam removal and was characterized by large deposits of legacy sediments. Courthouse Creek was characterized by sandy substrates typical of Coastal Plain streams. Sediment characteristics differed between the …


Characterizing The Distribution Of Planktonic Fecal Bacteria In The James River, Richmond, Virginia, John Furry Aug 2011

Characterizing The Distribution Of Planktonic Fecal Bacteria In The James River, Richmond, Virginia, John Furry

Theses and Dissertations

Surface waters containing fecal bacteria present significant public health risks. Understanding the sources of and factors affecting the distribution of fecal-indicating bacteria is necessary to predict potential illnesses more effectively. This thesis presents two studies on the distribution of fecal bacteria in the James River through Richmond, Virginia. Chapter 1 describes nearly 11 years of water quality, climate, and hydrologic data that occurred with changes in Escherichia coli concentrations, concluding that Richmond contributes significant quantities of fecal bacteria to the James River, and that the distribution of these bacteria varies seasonally. Chapter 2 details the development of Polymerase Chain Reaction …


Morphology, Material And Vibratory Properties Of The Swimbladder In The Carp, Cyprinus Carpio, Yasha Mohajer Jul 2011

Morphology, Material And Vibratory Properties Of The Swimbladder In The Carp, Cyprinus Carpio, Yasha Mohajer

Theses and Dissertations

The carp Cyprinus carpio has a two-chambered swimbladder and excellent hearing. I explored the hypothesis that the anterior chamber, which connects to Weberian ossicles, is adapted for hearing by testing both chambers for material properties. I also determined displacement and auditory responses to mechanical strikes. Wall stress is higher in the posterior, strain in the anterior and modulus lower in the anterior chamber. Strikes increase pressure followed by a variable rebound that rapidly decays. Displacement and sound amplitude increase with hammer force, and amplitude is similar in both chambers for within chamber strikes but lower across chambers. Normalized for equivalent …


Novel Methodology For Assessing Phytoplankton Response To Pco2 Enrichment In Fresh And Saltwater, Susan B. Gifford Jul 2011

Novel Methodology For Assessing Phytoplankton Response To Pco2 Enrichment In Fresh And Saltwater, Susan B. Gifford

Theses and Dissertations

Atmospheric CO2 emissions are on the rise and are expected to reach 780 parts per million by the year 2100. Research investigating the impacts of increasing CO2 is a relatively new field and the response of phytoplankton communities is largely unknown, especially in coastal and freshwater ecosystems where no CO2 manipulation studies have completed. The present study attempts to encourage uniformity in methods utilized in CO2 perturbation studies and identifies changes in phytoplankton abundance in freshwater (James River) and coastal ocean (Atlantic, Cape Hatteras) sites. A novel bubbling method to manipulate pCO2 was compared with the classic method of acid …


Investigation Of Pre- And Post-Zygotic Reproductive Barriers Between Two Host-Plant Complex Races Of The Parasitic Wasp Cotesia Congregata (Say) [Hymenoptera: Braconidae], Justin Bredlau Jul 2011

Investigation Of Pre- And Post-Zygotic Reproductive Barriers Between Two Host-Plant Complex Races Of The Parasitic Wasp Cotesia Congregata (Say) [Hymenoptera: Braconidae], Justin Bredlau

Theses and Dissertations

Investigations of host-associated differentiation of parasitoids have largely focused on the degree of molecular genetic differentiation, but a true test of species status must examine the mating patterns of differentiated populations to determine if they can interbreed in the wild and produce viable offspring. We examined possible mechanisms of isolation between two genetically distinct host-plant complex races of the braconid, Cotesia congregata, originating from hosts on tobacco and catalpa. We compared male responses to female pheromones, elements of male acoustic courtship signals, and breeding success between the two races. Males responded to pheromones from both sources and male courtship signals …


Changes In Leaf Morphology, Photosynthesis And Nitrogen Content In Two Coastal Shrubs, Elizabeth Kost May 2011

Changes In Leaf Morphology, Photosynthesis And Nitrogen Content In Two Coastal Shrubs, Elizabeth Kost

Theses and Dissertations

It is important to understand mechanisms that facilitate expansion of two common shrubs, Morella cerifera and Baccharis halimifolia in coastal environments. The purpose of my study was to investigate the physiological and structural changes that occur as leaves age. Photosynthesis, incident light, chlorophyll, and leaf C:N ratios were quantified for young, intermediate, and old leaves (distal, central and proximal leaves, respectively). Leaf structural differences were also compared. Leaves did not change morphologically with age. Light decreased with leaf age and during winter months. Photosynthesis showed no seasonal or age related patterns. Chlorophyll increased initially and then declined with age due …


Exploring The Functional Interaction Between Camk-Ii And P53, Raymond Lai Apr 2011

Exploring The Functional Interaction Between Camk-Ii And P53, Raymond Lai

Theses and Dissertations

Calcium (Ca2+)/calmodulin-dependent kinase 2 (CaMK-II) is a multifunctional member of a family of Ca2+/calmodulin-dependent serine/threonine protein kinases that respond to transient intracellular calcium signaling. CaMK-II has been reported to be involved with transcription regulation, cell motility, neuronal development, cell cycle regulation, and more recently early development of vertebrates (Easley et al., 2008; Rothschild et al., 2009; Francescatto et al., 2010). Through previous work in the lab using tandem mass spectrometry and “substrate-trapping mutants”, tumor suppressor protein 53 (p53) was identified as a novel CaMK-II binding partner in tissue culture. In this study, I sought to provide characterization of the functional …


Morphometric And Genetic Variation Among Populations Of The Southern Short-Tailed Shrew, Blarina Carolinensis, Located Along The Coastal Plain Of Virginia, Devon Kersten Jan 2011

Morphometric And Genetic Variation Among Populations Of The Southern Short-Tailed Shrew, Blarina Carolinensis, Located Along The Coastal Plain Of Virginia, Devon Kersten

Theses and Dissertations

The distribution of short-tailed shrews, Blarina carolinensis and B. brevicauda, demonstrates contiguous allopatry in peninsular situations in the Coastal Plain of Virginia, and in other areas. The distribution of these two species is unusual in that they do not exhibit syntopy except within a narrow corridor along the borders of their ranges. Interestingly, along the Coastal Plain of Virginia, B. carolinensis is generally found only at the distal ends of the peninsular areas, existing as local populations that appear to be isolated from each other and from the primary population that exists in south-central Virginia. It is thought that the …


Head-Start Juvenile And Adult Resident Eastern Box Turtles (Terrapene Carolina Carolina): Winter Ecology Including Microhabitat Selection, Temperature Tolerance And Philopatry, Amberly Moon Jan 2011

Head-Start Juvenile And Adult Resident Eastern Box Turtles (Terrapene Carolina Carolina): Winter Ecology Including Microhabitat Selection, Temperature Tolerance And Philopatry, Amberly Moon

Theses and Dissertations

The Eastern box turtle (Terrapene carolina carolina) is one of the most common reptiles in North America, but is soon to be threatened due to increased urbanization, disease, and the pet and food trades. In order to assist resource managers in choosing suitable sites for relocation studies, more information on the winter ecology of this species is necessary. We examined the temperature tolerance, microhabitat and degree of philopatry to hibernacula for juvenile and adult E. box turtles for two consecutive winters in Charles City Co, VA. The turtles were tracked via radio telemetry to record point locations during the active …


Determining Impediments To Gene Flow In A Natural Population Of Cornus Florida L. Cornaceae, Using Integrative Landscape Genetic Techniques, Crystal Meadows Jan 2011

Determining Impediments To Gene Flow In A Natural Population Of Cornus Florida L. Cornaceae, Using Integrative Landscape Genetic Techniques, Crystal Meadows

Theses and Dissertations

This study examined the impact intervening environment has on gene flow in the insect pollinated understory tree, Cornus florida L., by combining GIS and landscape genetic techniques (Least Cost Path Analysis, Circuit Theory, and Conditional Genetic Distance). Traditional population genetic analysis indicated pair-wise relatedness was significantly correlated to distance (Pearson; r = -0.312, P < 0.001) suggesting a spatial component to offspring relatedness. Dispersal throughout the study site was non-random, exhibiting a high degree of pollen pool structure due to restricted gene flow (Two-Generation Analysis; Φft = 0.161, P = 0.001). Forest structure was quantified in GIS layers representing coniferous canopies, mixed hardwood canopies, C. florida canopies, open understory (roads), and open understory/canopy due to tree removal. Of these layers, landscape isolation for the roads layer provided the best-fit model for describing genetic differentiation among sampled pollen pools (Mantel; r = 0.542, P = 0.001). These data also suggest that improved biological inferences can be gained by examining a range of landscape isolation models.