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Population Demography, Spatial Ecology, And Habitat Use Of The Florida Box Turtle (Terrapene Bauri) On A Barrier Island, Michael D. Mills Nov 2022

Population Demography, Spatial Ecology, And Habitat Use Of The Florida Box Turtle (Terrapene Bauri) On A Barrier Island, Michael D. Mills

USF Tampa Graduate Theses and Dissertations

Turtles are one the most threatened vertebrate groups in the world due to anthropogenic threats such as habitat loss and overexploitation. In addition to occupying a range that has been vulnerable to major habitat loss, the Florida box turtle (Terrapene bauri) is particularly at risk of overexploitation due to its popularity in the pet trade. Sanibel Island is a barrier island in southwest Florida that has experienced major habitat loss and is the site of a recent poaching event. In response to these threats, studies of both the population demography and spatial ecology were conducted on Sanibel’s Florida box turtle …


Reproduction In Relation To Heavy-Metal Contamination Along An Urbanization Gradient In Aninvasive Florida Lizard Species (Anolis Sagrei), Elizabeth Hauck Nov 2022

Reproduction In Relation To Heavy-Metal Contamination Along An Urbanization Gradient In Aninvasive Florida Lizard Species (Anolis Sagrei), Elizabeth Hauck

USF Tampa Graduate Theses and Dissertations

Environmental contamination is accumulating rapidly worldwide due to increasing urbanization rates. Heavy metals are known to be particularly toxic to organisms, to accumulate in biological systems, and to be found in higher concentrations in animals located in areas of high urban levels. However, the physiological impacts of these toxic heavy metals in wildlife remain largely unknown. Lizards are excellent bioindicator species for environmental contaminants due to their trophic level as mesopredators and their susceptibility of exposure via air, water, diet, and soil through egg deposition. To assess the impacts heavy-metal contamination has on reproductive parameters in lizards and how these …


Investigating Students' Interpretations Of Ecological Food Webs, Christopher Neil Grissett Nov 2022

Investigating Students' Interpretations Of Ecological Food Webs, Christopher Neil Grissett

USF Tampa Graduate Theses and Dissertations

To better prepare undergraduate students for current and future biological challenges, scientists, educators and researchers in the Vision and Change report recommended five core conceptual areas essential for the improvement of biological literacy, one of which is biological systems. Systems are an identified core concept that may help promote biological literacy. One example of a system that students have difficulty understanding is an ecological food web, which consists of parts or components interacting with one another to perform a given phenomenon. The intricacies of this system tend to confuse students and can produce naïve conceptions that could hinder future learning …


Utilizing Maternal Investment Strategies Of Sharks To Develop Management Tools To Aid In Shark Conservation, Dennis Deeken Oct 2022

Utilizing Maternal Investment Strategies Of Sharks To Develop Management Tools To Aid In Shark Conservation, Dennis Deeken

USF Tampa Graduate Theses and Dissertations

Every generation, breeding females and males repopulate ecosystems with the next generation of offspring. Two models link maternal investments in offspring size and number to natural selection processes. In unstable resource environments, bet-hedging models predict a conservative tradeoff in maternal investments resulting in moderate egg size and clutch size within a given range of sizes for a given species. r/K selection models make predictions on maternal investments across species. In unstable resource environments, r-selected species invest in large broods of small offspring. In stable environments, K-selected species invest in small broods of large offspring. The maternal risk management model predicts …


Interactions Between Juvenile Estuary-Dependent Fishes And Microalgal Dynamics, Ian C. Williams Oct 2022

Interactions Between Juvenile Estuary-Dependent Fishes And Microalgal Dynamics, Ian C. Williams

USF Tampa Graduate Theses and Dissertations

The ontogenetic movement of juvenile estuary-dependent fishes upstream in estuarine settings is a phenomenon observed around the world. These movements usually coincide with a shift in diet from smaller prey, such as calanoid copepods, to larger prey, such as mysids. However, the mechanism that drives this pattern has yet to be conclusively described. Prior to the current study, zooplankton/hyperbenthos (“zooplankton”), primary production, and water quality data were collected from the Caloosahatchee River estuary in two concurrent and coordinated studies over a two-year period. One of the products of these sampling efforts was a classification of primary-producer types at the sediment-water …


A Holistic Investigation Of Acidosis In Breast Cancer, Bryce Ordway Oct 2022

A Holistic Investigation Of Acidosis In Breast Cancer, Bryce Ordway

USF Tampa Graduate Theses and Dissertations

In the early 20th century, Nobel laureate Otto Warburg made the observation that cells of a carcinoma had considerably higher glycolytic metabolism and considerably lower oxidative metabolism compared to cells of a normal tissue. He postulated that within this observation was the key to deciphering the differences between malignant and normal tissue. It is now well established that tumors of the breast are unequivocally acidic, caused by an abnormal amount of aerobic glycolysis, colloquially known as the Warburg effect. Over the last decades, our group, led by Dr. Robert J. Gillies, has set out to characterize the causes and consequences …


X-Linked Ubiquitin-Specific Peptidase 11 Increases Tauopathy Vulnerability In Women, Yan Yan Oct 2022

X-Linked Ubiquitin-Specific Peptidase 11 Increases Tauopathy Vulnerability In Women, Yan Yan

USF Tampa Graduate Theses and Dissertations

Women experience significantly higher tau burden and increased risk for Alzheimer’s disease (AD) than men, yet the underlying mechanism for this vulnerability has not been explained. Here, we demonstrate through in vitro and in vivo models, as well as human AD brain tissue, that X-linked ubiquitin specific peptidase 11 (USP11) augments pathological tau aggregation via tau deubiquitination initiated at lysine-281. Removal of ubiquitin provides access for enzymatic tau acetylation at lysines 281 and 274. USP11 escapes complete X-inactivation, and female mice and people both exhibit higher USP11 levels than males. Genetic elimination of usp11 in a tauopathy mouse model preferentially …


Regulation Of The Heat Shock Response Via Lysine Acetyltransferase Cbp-1 And In Neurodegenerative Disease In Caenorhabditis Elegans, Lindsey N. Barrett Jul 2022

Regulation Of The Heat Shock Response Via Lysine Acetyltransferase Cbp-1 And In Neurodegenerative Disease In Caenorhabditis Elegans, Lindsey N. Barrett

USF Tampa Graduate Theses and Dissertations

The decline of proteostasis is a hallmark of aging that is, in part, affected by the dysregulation of the heat shock response (HSR), a highly conserved cellular response to proteotoxic stress in the cell. The heat shock transcription factor HSF-1 is well-studied as a key regulator of proteostasis, but mechanisms that could be used to modulate HSF-1 function to enhance proteostasis during aging are largely unknown. In this study, we examined lysine acetyltransferase regulation of the HSR and HSF-1 in C. elegans. We performed an RNA interference screen of lysine acetyltransferases and examined mRNA expression of the heat-shock inducible gene …


Comparing Brood Care Behaviors Across Five Fertility Castes In The Invasive Fire Ant,Solenopsis Invicta,And The Expression Of Inotocin, Alexis Masnjak Jun 2022

Comparing Brood Care Behaviors Across Five Fertility Castes In The Invasive Fire Ant,Solenopsis Invicta,And The Expression Of Inotocin, Alexis Masnjak

USF Tampa Graduate Theses and Dissertations

The fire ant, Solenopsis invicta, is one of the most successful of the invasive eusocial invertebrates. The fire ant’s prolific reproductive capacity and its division of labor among five fertility castes are key factors to its successful invasion and global colonization across all continents except Antarctica. Although the rate of self-care and brood care by sterile S. invicta workers is well known, little is known about the rate of self-care and brood care by the other four fertility castes. In this study, I quantified the frequency and duration of behaviors by alate males, alate queens, newly mated queens, older mature …


Antibiotic-Resistant Escherichia Coli And EnterococcusSpp. In Sand And Water At Tampa Bay Beaches, Jennifer K. Sabater Jun 2022

Antibiotic-Resistant Escherichia Coli And EnterococcusSpp. In Sand And Water At Tampa Bay Beaches, Jennifer K. Sabater

USF Tampa Graduate Theses and Dissertations

As antibiotic resistance in the environment continues to rise there is an increased concern that infections may become harder to treat as bacteria acquire genes for multidrug resistance. Recreational beach waters in the Tampa Bay area are routinely monitored by the Florida Department of Environmental Protection for the presence of fecal indicator bacteria (FIB) such as Escherichia coli and enterococci. Exceedances of beach action values (BAV) 235 CFU/100 mL (E. coli) and 70 CFU/100 mL (enterococci) indicate the presence of fecal contamination which is associated with an increased risk of disease for beachgoers. Antibiotic-resistant E. coli and Enterococcus spp. have …


Continuous Effort Required To Maintain Populations Of Outplanted Acropora Cervicornis In The Florida Reef Tract, Usa, Tiffany S. Boisvert Jun 2022

Continuous Effort Required To Maintain Populations Of Outplanted Acropora Cervicornis In The Florida Reef Tract, Usa, Tiffany S. Boisvert

USF Tampa Graduate Theses and Dissertations

The degradation of coral reefs due to natural and anthropogenic stressors has resulted in the expansion of coral restoration projects worldwide. In the Caribbean region, most restoration efforts focus on outplanting Acropora cervicornis, once a dominant branching coral, now found predominantly in spatially isolated populations. Thousands of A. cervicornis colonies are propagated within nurseries and outplanted onto degraded reefs every year. However, monitoring the long-term growth and survival of outplanted corals has been limited by financial, physical, and temporal constraints. In the current study, we assessed the long-term success of A. cervicornis restoration by determining the relationship between current populations …


Mixed Method Analysis Of Undergraduate Student Interpretation Of Different Phylogenetic Trees, Faith Frings Jun 2022

Mixed Method Analysis Of Undergraduate Student Interpretation Of Different Phylogenetic Trees, Faith Frings

USF Tampa Graduate Theses and Dissertations

Phylogenetic trees are common tools used to visualize evolutionary concepts such as historical patterns of ancestry, divergence of species, and descent of species. However, students have misconceptions when reading these abstract diagrams. The purpose of this study was to compare student performance and evolutionary thinking when using two styles of phylogenetic trees: cladograms and phylograms. The study also assessed the validity of a hierarchal theoretical framework evaluating student phylogenetic tree interpretation. Introductory biology students from two research universities were assigned to two groups, one solely given assessments with phylograms, and one solely given assessments with cladograms. One-on-one student interviews were …


Host-Pathogen Coevolution Between Tasmanian Devils (Sarcophilus Harrisii) And Devil Facial Tumor Disease, Dylan Garret Gallinson Apr 2022

Host-Pathogen Coevolution Between Tasmanian Devils (Sarcophilus Harrisii) And Devil Facial Tumor Disease, Dylan Garret Gallinson

USF Tampa Graduate Theses and Dissertations

Coevolution is a driving force of rapid evolution, yet the complexity of coevolutionary interactions has made it difficult to characterize the genomic basis of traits mediating such relationships. Coevolutionary dynamics are especially important in host-pathogen systems where the host and pathogen must constantly adapt to one another. The Tasmanian devil and its species-specific transmissible cancer, devil facial tumor disease (DFTD), provide the rare opportunity to study host-pathogen coevolution in a complex natural system. Extensive spatiotemporal devil sampling, high linkage disequilibrium in devils, and a large selective pressure imposed by DFTD facilitate a system tractable for study. Here, we characterized devil …


Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven Mar 2022

Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven

USF Tampa Graduate Theses and Dissertations

Bolinopsis infundibulum is a wide-ranging, ubiquitous ctenophore whose fragile nature makes the collection of specimens and quantification of key predator-prey activities in controlled laboratory experiments, challenging. Thus, in situ methods often represent the best means for data collection. However, while present in surface waters, these animals can also be abundant at depths well beyond those attainable by divers. As a result, very little empirical data exist over the depth range of their natural habitats which limits our ability to assess key predator-prey interactions needed to assess their ecological role in midwater food webs. Working in Monterey Bay, California, remotely operated …


Characterizing The Impact Of Postharvest Temperature Stress On Polyphenol Profiles Of Red And White-Fruited Strawberry Cultivars, Alyssa N. Smith Jan 2022

Characterizing The Impact Of Postharvest Temperature Stress On Polyphenol Profiles Of Red And White-Fruited Strawberry Cultivars, Alyssa N. Smith

USF Tampa Graduate Theses and Dissertations

All living organisms have developed mechanisms that help them prevent internal stress and survive under harsh conditions. For fruits, specifically strawberries, stress is fought against primarily by increasing the synthesis of polyphenols, which are secondary plant metabolites with bioactive activity. Characterizing these bioactive compounds and the differences between strawberry cultivars can be vital for strawberry breeders. Furthermore, understanding the mechanisms that trigger the synthesis of polyphenols and their levels within different strawberry cultivars will provide breeders with tools to successfully identify cultivars with higher resistance to pre- and postharvest stressors. To gain that understanding, this study aimed to characterize the …