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The Effect Of Thyroid Hormone On The Expression Of A7 Myosin Heavy Chain Mrna In Xenopus Laevis, Bradley Thomas Butkovich
The Effect Of Thyroid Hormone On The Expression Of A7 Myosin Heavy Chain Mrna In Xenopus Laevis, Bradley Thomas Butkovich
Master's Theses
It has long been known that thyroid hormone (TH) is responsible for metamorphosis in tadpoles. It is also known that A7 MHC, an adult muscle isoform, is expressed in adult Xenopus laevis frogs, but not in larvae. Furthermore, the appearance of A7 corresponds with the onset of metamorphosis. The present study investigated the relationship between TH and the expression of A7 MHC mRNA in Xenopus laevis. Isolated tails in culture and 6-n-propyl 2-thiouracil (PTU) treated tails were treated with TH and the presence of A7 mRNA analyzed with in-situ hybridization, and RT-PCR. It was found that TH caused substantial …
Some Factors Involved In Growth And Sporulation Of Pilobolus Crystallinus Tode And Pilobolus Umbonatus Buller, Kenneth L. Poff
Some Factors Involved In Growth And Sporulation Of Pilobolus Crystallinus Tode And Pilobolus Umbonatus Buller, Kenneth L. Poff
Master's Theses
Pilobolus umbonatus Buller and P. crystallinus Tode grew normally in a nitrogen atmosphere but their sporagnia were non-pigmented. When palced in anaerobic jars along with obligately anaerobic and obligately aerobic bacteria, the fungi grew in the range of oxygen concentrations too low for growth of the aerobic bacterium, but high enough to prevent growth of the anaerobe. The fungi did not grow when the oxygen was depleted to the point that allowed growth of the anaerobic bacterium. Catalase was not produced by the hyphae and trophocysts which normally grow beneath the surface of the medium, but was present in the …