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Cd46 Interaction With Adenovirus Type 64, A Causative Agent For Viral Pink Eye, Hanglin (Henry) Zhu

Honors Theses

Human adenovirus type 64 (Ad64) is a causative agent of contagious viral pink eye. We do not understand why Ad64 causes eye infections. One likely reason is the presence of a receptor molecule on the exposed surface of eye cells, but not other cells. Ad64 binds to a protein called CD46 on the cell surface. We aim to visualize how CD46 binds to the surface of Ad64. I mixed the extracellular portion of CD46 with purified Ad64 virus, then visualized it using negative stain transmission electron microscopy. In order to gain insight into how the virus binds CD46, we used …

Investigation Of The Vira Linker Domain To Characterize Its Phenol Interactions, Jessica Garofalo

Honors Theses

The soil bacterium Agrobacterium tumefaciens causes tumors in plants through interkingdom gene transfer. This transfer is initiated upon a wounding event that results in the release of plant signaling factors such as phenols and sugars that are recognized by protein machinery in the periplasm and inner membrane of A. tumefaciens. The histidine kinase protein VirA, in combination with the periplasmic protein ChvE, recognizes these signals and initiates induction of virulence genes via a signaling pathway, culminating with the insertion of tumor-inducing T-DNA into the wounded plant cells. While the interaction between the Periplasmic domain of VirA and sugar-bound ChvE …

Analysis Of Wild Rodent Gut Microbiota As A Function Of Exposure To Ticks And Tick-Borne Pathogens, Joshua Pandian

Honors Theses

Due to advances in high-throughput parallel sequencing, researchers have conducted novel studies exploring relationships between microbiome compositions and different aspects of organism health. Some of these studies have shown that the gut microbiome of rodent models has effects on organism health and behavior and that infection with pathogens and the composition of the skin microbiome are linked to changes in gut microbiome composition. While previous studies have shown how vector microbiota impact vector behavior and pathogen transmission, the effect vectors have on reservoir species microbiomes has been a less prominent focus. We were interested in the relationships between tick parasitism, …

Myo19 Tethers Mitochondria To Endoplasmic Reticulum-Associated Actin To Promote Mitochondrial Fission, Stephen M. Coscia, Cameron P. Thompson, Qing Tang, Elana E. Baltrusaitis, Joseph A. Rhodenhiser, Omar A. Quintero, E. Michael Ostap, Melike Lakadamyali, Erika L. Holzbaur

Biology Faculty Publications

Mitochondrial homeostasis requires a dynamic balance of fission and fusion. The actin cytoskeleton promotes fission, and we found that the mitochondrially localized myosin, myosin 19 (Myo19), is integral to this process. Myo19 knockdown induced mitochondrial elongation, whereas Myo19 overexpression induced fragmentation. This mitochondrial fragmentation was blocked by a Myo19 mutation predicted to inhibit ATPase activity and strong actin binding but not by mutations predicted to affect the working stroke of the motor that preserve ATPase activity. Super-resolution imaging indicated a dispersed localization of Myo19 on mitochondria, which we found to be dependent on metaxins. These observations suggest that Myo19 acts …

Halogen Bonding: A Computational Chemistry Investigation Of The Interaction Between Thyroid Hormone And Deiodinase, William Rice

Honors Theses

Halogen bonding is a noncovalent interaction that continues to garner interest among the scientific community. Investigation of halogen bonds in biological contexts typically revolves around rational drug design for developing therapeutics. However, halogen bonding may be occurring naturally in our body every day. Thyroid hormone and its regulating enzyme, iodothyronine deiodinase, show promising results for a halogen bonding interaction that happens during catalysis. Prior work has examined the interaction between the iodine of thyroid hormone and the selenium of iodothyronine deiodinase. However, this study is the first of its kind to use computational chemistry methods to analyze the halogen bond …

Investigation Of The Effects Of Resveratrol And Epigallocatechin Gallate On Woodsmoke-Induced Inflammation, Sarenna Naomi Enright

Honors Theses

Until a few years ago, most scientific investigations related to the health effects of air pollution focused on outdoor air pollutants. But in recent years, the concerns over indoor air pollution has increased. People can spend up to 90% of their time in indoor environments, especially their homes, even more so since the beginning of the COVID-19 pandemic. The concentrations of some air pollutants are five times higher than what is found outdoors.

Indoor air pollution exposure remains a significant cause of morbidity and mortality worldwide. Approximately half of the global population is exposed to abnormally high concentrations of household …

Larval Chondrocranial And Internal Oral Morphology Of The Neotropical Treefrog Boana Crepitans (Wied-Neuwied, 1824; Amphibia, Anura, Hylidae), Marianna Isabella Rosa Rodrigues De Oliveira, Luiz Norberto Weber, Johnny Sousa Ferreira, Anna Evelin Coimbra Liborio, André Masahide Guimaraes Takazone, Rafael O. De Sá

Biology Faculty Publications

We describe the internal oral morphology and chondrocranial anatomy for Boana crepitans tadpoles, and compare them with available descriptions for other species in the subfamily Cophomantinae. Among species of the Boana faber group, the chondrocranial anatomy has been reported only for one species internal oral morphology and cranial anatomy are similar to other described species of Boana and Cophomantinae. B. crepitans lacks unique features in the oral cavity and chondrocranium that would distinguish it from other congeneric species. We identify six characters from the internal oral anatomy of tadpoles unique for Cophomantinae. In addition, Boana has infralabial papillae projections, buccal …

Research Towards Understanding Hox Regulation By Cdx, Joshua M. Fisher

Honors Theses

In this project, we aim to functionally test the contribution that individual Cdx binding sites have in hox gene regulation. We will achieve this by individually blocking Cdx binding sites using CRISPR/dCas9 in the zebrafish and then analyzing changes in the time, distribution, and levels of hox gene transcription.

We will focus our analysis in only one of the seven zebrafish hox clusters, the hoxca cluster. We are focusing on the hoxca gene cluster because it has lost the least number of genes relative to other clusters and is involved in specifying the axial identity of cells in …

Investigation Of The Effects Of Curcumin On Woodsmoke-Induced Inflammation, Melissa Lewis

Honors Theses

Inflammatory diseases caused by biomass smoke exposure and indoor air pollution affect millions of people worldwide. These diseases can be caused by the burning of biomass (plastic, wood, rubber, tobacco, etc.) for light or heat. Acrolein is a common indoor and outdoor pollutant from tobacco smoke or organic combustion. Wood smoke is hypothesized to cause inflammation since it contains high concentrations of particulate matter and gaseous compounds and are similar in size to other well-known damaging particles. Natural remedies, like curcumin, are hypothesized to be a natural remedy for combating inflammation. The goal of this research was to investigate the …

The Effect Of Color Morph On Behavior Interactions In Red-Backed Salamanders (Plethodon Cinereus), Taylor M. Aliferis

Honors Theses

Studies in behavioral ecology provide critical information regarding a species’ territoriality, predator-prey interactions, and reproduction. Red-backed salamanders (Plethodon cinereus) are a polymorphic terrestrial amphibian whose behavioral interactions have long been studied to provide insights on the processes that structure salamander populations, which are often cryptic and difficult to study. However, research regarding the effect of coloration on these interactions is more limited, but potentially extremely important for studies of population dynamics and speciation in terrestrial salamanders. In this study, aggressive and submissive behaviors of adult male red-backed salamanders in five different color morph treatment pairings were observed to …

Characterization Of A Myo19 Knockdown Phenotype In A Cultured Neuron-Like Cell Line, Adelaide Masterson, Bridget Wolters

Arts & Sciences Student Symposium

We will be characterizing and analyzing properties related to neurite outgrowth, including neurite length & mitochondrial dynamics, in order to understand how MYO19 activity and mitochondrial distribution play a role in neurite development. We aim to observe if loss of MYO19 expression alters these properties, and if reintroducing specific aspects of MYO19 function can “rescue” any observed defects in cells where normal MYO19 expression has been lost. Our studies and hypothesis are based on the previous work of Dr. Quintero and his students, where they have observed differences in the behavior of neuron-like CAD cells when MYO19 expression has been …

Larval Anatomy Of Monotypic Painted Ant Nest Frogs Lithodytes Lineatus Reveals Putative Homoplasies With The Leptodactylus Pentadactylus Group (Anura: Leptodactylidae), Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The morphological diversity of anuran larvae made them an important source of information for evolutionary and systematic studies. For the monotypic frog genus Lithodytes, which has an interesting taxonomic history, including its past synonymizing with Adenomera and its placement as a subgenus of Leptodactylus, the information provided from its larvae can help to understand its systematics interrelationships and also provide insights about its evolutionary trajectories. Herein, we provide a detailed description of the larval morphology of Lithodytes lineatus, including novel data of internal morphology (buccopharyngeal cavity and skeleton), and discuss some morphological and evolutionary aspects in relation …

Taxonomic Review Of South American Butter Frogs: Phylogeny, Geographic Patterns, And Species Delimitation In The Leptodactylus Latrans Species Group (Anura: Leptodactylidae), Felipe De M. Magalhães, Mariana L. Lyra, Thiago R. De Carvalho, Diego Baldo, Francisco Brusquetti, Pamela Burella, Guarino R. Colli, Marcelo C. Gehara, Rafael O. De Sá, Et Al.

Biology Faculty Publications

The Leptodactylus latrans species group currently comprises eight medium- to large-sized frog species with a convoluted taxonomic history, particularly related to the specific limits of the L. latrans complex, and the species pair Leptodactylus chaquensis–Leptodactylus macrosternum. Their homogeneous external morphology and continental geographic distribution in South America have posed severe limitations to a comprehensive review, such that taxonomic consensus and species limits remain uncertain. This is further worsened by the presence of chromatic polymorphism among coexisting species that can hardly be distinguished by external morphology. Based on a large-scale geographic sampling including multilocus DNA analyses, and acoustic …

Climate-Related Geographical Variation In Performance Traits Across The Invasion Front Of A Widespread Non-Native Insect, Lily M. Thompson, Sean D. Powers, Ashley Appolon, Petra Hafker, Leila Milner, Dylan Parry, Salvatore J. Agosta, Kristine L. Grayson

Biology Faculty Publications

Aim

Invasive species are ideal systems for testing geographical differences in performance traits and measuring evolutionary responses as a species spreads across divergent climates and habitats. The European gypsy moth, Lymantria dispar dispar L. (Lepidoptera: Erebidae), is a generalist forest defoliator introduced to Medford, Massachusetts, USA in 1869. The invasion front extends from Minnesota to North Carolina and the ability of this species to adapt to local climate may contribute to its continuing spread. We evaluated the performance of populations along the climatic gradient of the invasion front to test for a relationship between climate and ecologically important performance traits. …

Tadpole Of The Amazonia Frog Edalorhina Perezi (Anura: Leptodactylidae) With Description Of Oral Internal And Chondrocranial Morphology, Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The genus Edalorhina consists of two species of small forest-floor frogs inhabiting the Amazon basin. The tadpole of Edalorhina perezi, the most widely distributed species, was previously described based on a single and early stage (Gosner 25) individual. Herein, we provide a description of the tadpole in Gosner stages 35–36 including internal morphology data (i.e., buccopharyngeal cavity and larval skeleton) based on samples from two populations from Ecuador. Edalorhina shares a generalized morphology with most members of its closely related taxa; however, it is distinguished from the other species by having an almost terminal oral disc. The presence of …

The Importance Of Ile716 Toward The Mutagenicity Of 8-Oxo-2’-Deoxyguanosine With Bacillus Fragment Dna Polymerase, Michelle L. Hamm, Anarosa A. Garcia, Rachel Gilbert, Manavi Johri, Miranda Ricart, Samantha L. Sholes, Laura A. Murray-Nerger, Eugene Y. Wu

Biology Faculty Publications

8-oxo-2’-deoxyguanosine (OdG) is a prominent DNA lesion that can direct the incorporation of dCTP or dATP during replication. As the latter reaction can lead to mutation, the ratio of dCTP/dATP incorporation can significantly affect the mutagenic potential of OdG. Previous work with the A-family polymerase BF and seven analogues of OdG identified a major groove amino acid, Ile716, which likely influences the dCTP/dATP incorporation ratio opposite OdG. To further probe the importance of this amino acid, dCTP and dATP incorporations opposite the same seven analogues were tested with two BF mutants, I716M and I716A. Results from these studies support the …

Spatiotemporal Variability In Allee Effects Of Invading Gypsy Moth Populations, Jonathan A. Walter, Kristine L. Grayson, Laura M. Blackburn, Patrick C. Tobin, Derek M. Johnson

Biology Faculty Publications

The Allee threshold, the critical population density separating growth from decline in populations experiencing strong Allee effects, can vary over space and time but few empirical studies have examined this variation. A lack of geographically extensive, long-term studies on low density population dynamics makes studying variability in Allee effects difficult. We used North American gypsy moth population data from 1996-2016 to quantify Allee thresholds in 11 regions of the invasion front. Allee thresholds spanned a continuum from being undetectable due to strong population growth at all densities, to being unmeasurable because populations declined across all densities. The lag-1 temporal autocorrelation …

The Influence Of Flooding On Macroinvertebrate Diversity Of The James River Rock Pools, Meghan Leber

Honors Theses

Biodiversity within an ecosystem can be greatly influenced by environmental disturbances, such as fires, flooding, or other extreme events. Studying the effects of these disturbances on species diversity can be complicated though, due to difficulties tracking species-level responses and isolating the effects of disturbances. Model systems in community ecology, such as rock pools, are a beneficial way to scale down the study of disturbances in discrete communities without losing the ability to analyze important influences or interactions. In this study, macroinvertebrates within the James River rock pools were surveyed to investigate seasonal and annual differences in species richness and diversity, …

Thermal Stress Responses Of Sodalis Glossinidius, An Indigenous Bacterial Symbiont Of Hematophagous Tsetse Flies, Jose Santinni Roma, Shaina D’Souza, Patrick J. Somers, Leah F. Cabo, Ruhan Farsin, Serap Aksoy, Laura J. Runyen-Janecky, Brian L. Weiss

Biology Faculty Publications

Tsetse flies (Diptera: Glossinidae) house a taxonomically diverse microbiota that includes environmentally acquired bacteria, maternally transmitted symbiotic bacteria, and pathogenic African trypanosomes. Sodalis glossinidius, which is a facultative symbiont that resides intra and extracellularly within multiple tsetse tissues, has been implicated as a mediator of trypanosome infection establishment in the fly’s gut. Tsetse’s gut-associated population of Sodalis are subjected to marked temperature fluctuations each time their ectothermic fly host imbibes vertebrate blood. The molecular mechanisms that Sodalis employs to deal with this heat stress are unknown. In this study, we examined the thermal tolerance and heat shock response of …

Presence And Distribution Of Leptodactylus Guianensis Heyer And De Sa, 2011 In Colombia: Comparisons With Other Species In The L. Latrans Group., Mariela Osorno Muñoz, Doris L. Gutiérrez-Lamus, Rafael O. De Sá

Biology Faculty Publications

Leptodactylus guianensis is reported for the first time for Colombia along with a distributional map of the species. The species is the fifth species of the L. latransspecies group documented in the country. Males possess a single, conical and slightly chisel-shaped thumb spine; only three species in the group have a single thumb spine. In collections, specimens of L. guianensis are misidentified as L. macrosternum, L. latrans or L. bolivianus. We provide morphological, colouration and habitat descriptions for where the species occurs in Colombia.

[Introduction To] Collateral Values: The Natural Capital Created By Landscapes Of War., Todd R. Lookingbill, Peter D. Smallwood

Bookshelf

This book explores the unanticipated benefits that may arise after wars and conflicts, showing how the preservation of battlefields and the establishment of borderlands can create natural capital in the former landscapes of war. The editors call this Collateral Value, in contrast to the collateral damage that war inflicts upon infrastructure, natural capital, and human capital. The book includes case studies recounting successes and failures, opportunities and risks, and ambitious proposals.

The book is organized in two sections. The first visits U.S., English, and French battlefield sites dating from medieval England to World War I. The second explores borderlands located …

Retinoic Acid Inhibition Impairs Planarian Eye Regeneration, Gabrielle Bennetti, Aaron Corbin-Leftwich, Linda M. Boland, Isaac Skromne

Biology Faculty Publications

Retinoic acid is a known morphogen in regulating animal growth and development. Planaria are a key model system for regeneration and their eyes are a morphological marker of anterior differentiation. We explored the requirement for retinoic acid signaling in the regeneration of body parts in the planaria S. mediterranea using an inhibitor of retinoic acid synthesis, diethylaminobenzaldehyde (DEAB). Whole planaria, soaked in DEAB for three days prior to and five days following amputation, produced trunk and tail fragments with defective anterior regeneration. Following regeneration, up to 80% of posterior fragments developed abnormal eyes. The abnormalities included animals without eyes, with …

What Lies Beneath? : Removal Sampling To Test For Biases In Surface Activity Of The Eastern Red-Backed Salamander (Plethodon Cinereus), Amelia Tedesco

Honors Theses

In light of the current global amphibian extinction crisis from threats such as climate change and disease, reliable and repeatable sampling methods carry heavy importance for assessing North American salamander species.

Multiple Connections Between Amazonia And Atlantic Forest Shaped The T Phylogenetic And Morphological Diversity Of Chiasmocleis Mehely, 1904 (Anura: Microhylidae: Gastrophryninae), Rafael O. De Sá, João Filipe Riva Tonini, Hannah Van Huss, Alex Long, Travis Cuddy, Mauricio C. Forlani, Pedro L.V. Peloso, Hussam Zaher, Célio F.B. Haddad

Biology Faculty Publications

Chiasmocleis is the most species-rich genus of Neotropical microhylids. Herein, we provide the first comprehensive multilocus phylogeny for the genus, including all but 3 of the 34 recognized species and multiple individuals per species. We discuss cryptic speciation, species discovery, patterns of morphological evolution, and provide a historical biogeographic analysis to account for the current distribution of the genus. Diversification of Chiasmocleis from other New World microhylids began during the Eocene, app. 40 mya, in forested areas, and current diversity seems to be a product of recurrent connections between the Atlantic Forest and Amazonia. Small-sized species evolved independently three times …

Review Of The Family Rivulidae (Cyprinodontiformes, Aplocheiloidei) And A Molecular And Morphological Phylogeny Of The Annual Fish Genus Austrolebias Costa 1998, Marcelo Loureiro, Rafael O. De Sá, Sebastián W. Serra, Felipe Alonso, Luis Esteban Krause Lanés, Matheus Vieira Volcan, Pablo Calviño, Dalton Nielsen, Alejandro Duarte, Graciela Garcia

Biology Faculty Publications

The family Rivulidae is the fourth most diverse clade of Neotropical fishes. Together with some genera of the related African family Nothobranchiidae, many rivulids exhibit a characteristic annual life cycle, with diapausing eggs and delayed embryonic development, which allows them to survive in the challenging seasonal ponds that they inhabit. Rivulidae also includes two species known as the only the self-fertilizing vertebrates and some species with internal fertilization. The first goal of this article is to review the systematics of the family considering phylogenetic relationships and synapomorphies of subfamilial clades, thus unifying information that is dispersed throughout the literature. From …

First Record Of The Genus Leptodactylus (Anura: Leptodactylidae) In Cuba: Leptodactylus Fragilis, A Biological Invasion?, Tomás M. Rodríguez-Cabrera, L. Yusnaviel García-Padrón, Andrés R. Acosta Galvis, Rafael O. De Sá, Roberto Alonso Bosch

Biology Faculty Publications

The Neotropical genus Leptodactylus is currently represented by three species in the West Indies (Leptodactylus albilabris, Leptodactylus fallax and Leptodactylus validus). Based on morphological, acoustic and molecular evidence, we document the presence of a fourth species in the Caribbean region, Leptodactylus fragilis (Brocchi, 1877). The species was found at two localities in western Cuba, and molecular data suggest a northern South American origin, possibly Venezuela, for these populations. We discuss the potential invasive status of L. fragilis, based on its known distribution, relative abundance, behaviour and possible impacts on native species of Cuban amphibians.

Cross-Life Stage Effects Of Aquatic Larval Density And Terrestrial Moisture On Growth And Corticosterone In The Spotted Salamander, Julie F. Charbonnier, Jacquelyn Pearlmutter, James R. Vonesh, Caitlin R. Gabor, Zachery R. Forsburg, Kristine L. Grayson

Biology Faculty Publications

For organisms with complex life cycles, conditions experienced during early life stages may constrain later growth and survival. Conversely, compensatory mechanisms may attenuate negative effects from early life stages. We used the spotted salamander, Ambystoma maculatum, to test how aquatic larval density and terrestrial moisture influence juvenile growth, food intake, evaporative water loss and water reuptake rates, and corticosterone levels. We conducted an outdoor mesocosm experiment to manipulate larval density and transferred metamorphosed salamanders into low and high terrestrial moisture treatments in laboratory terrariums. After the larval stage, high-density salamanders were significantly smaller and had higher corticosterone release rates …

The Hero Organism And Heroism Science: A New Frontier Of Human Consciousness, Olivia Efthimiou

Heroism Science

In the context of a new science of heroism this article presents a brief history of the presence and study of heroism, and an outline of the key characteristics of the modern-day movement of heroism. The potentially wide-reaching impacts of the science of heroism are briefly discussed. The hypothesis of human beings as “hero organisms” is presented, before embarking on a discussion on what it means for each of us to be on our own hero’s journey.

The Search For A Hero Gene: Fact Or Fiction?, Olivia Efthimiou

Heroism Science

The radical entry of heroism research into scientific inquiry presents interesting challenges and possibilities for the study of heroism and the human condition more broadly. This ‘final frontier’ of the enduring phenomenon of heroism stands to offer remarkable, unprecedented, and controversial advances in our understanding of heroic and human behaviour. Is a genetic basis for heroism a real possibility? If so, what would its impacts be? Advances in genomics and increased interest in the fields of epigenetics and neuroplasticity might hold the key to its discovery. This article considers some of the leading emerging research in global health genomics and …

Thermal Physiology And Developmental Plasticity Of Pigmentation In The Harlequin Bug (Hemiptera: Pentatomidae), Carly D. Sibilia, Kelly A. Brosko, Christopher J. Hickling, Lily M. Thompson, Kristine L. Grayson, Jennifer R. Olson

Biology Faculty Publications

Traits that promote the maintenance of body temperatures within an optimal range provide advantages to ectothermic species. Pigmentation plasticity is found in many insects and enhances thermoregulatory potential as increased melanization can result in greater heat retention. The thermal melanism hypothesis predicts that species with developmental plasticity will have darker pigmentation in colder environments, which can be an important adaptation for temperate species experiencing seasonal variation in climate. The harlequin bug (Murgantia histrionica, Hemiptera: Pentatomidae, Hahn 1834) is a widespread invasive crop pest with variable patterning where developmental plasticity in melanization could affect performance. To investigate the impact of temperature …