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Substrate And Regulation Of Mitochondrial Μ-Calpain, Aashish Joshi
Substrate And Regulation Of Mitochondrial Μ-Calpain, Aashish Joshi
University of Kentucky Doctoral Dissertations
μ -Calpain is localized to the mitochondrial intermembrane space. Apoptosisinducing factor (AIF), which executes caspase-independent cell death, is also localized to the mitochondrial intermembrane space. Following processing at the N-terminus, AIF becomes truncated (tAIF) and is released from mitochondria. The protease responsible for AIF processing has not been established. The same submitochondrial localization of mitochondrial μ-calpain and AIF gives support to the hypothesis that mitochondrial μ-calpain may be responsible for processing AIF. Atractyloside-induced tAIF release in rat liver mitochondria was inhibited by cysteine protease inhibitor MDL28170, but not by calpain inhibitors PD150606 or calpastatin. Moreover, μ-calpain immunoreactivity was difficult to …
Physiological Genomics Of Spinal Cord And Limb Regeneration In A Salamander, The Mexican Axolotl, James Robert Monaghan
Physiological Genomics Of Spinal Cord And Limb Regeneration In A Salamander, The Mexican Axolotl, James Robert Monaghan
University of Kentucky Doctoral Dissertations
Salamanders have a remarkable ability to regenerate complex body parts including the limb, tail, and central nervous system. Although salamander regeneration has been studied for several hundred years, molecular-level studies have been limited to a relatively few important transcription factors and signaling molecules that are highly conserved among animals. Physiological genomic approaches were used here to investigate spinal cord and limb regeneration. Chapter 2 reports that hundreds of gene expression changes were identified during spinal cord regeneration, showing that a diverse injury response is activated in concert with extracellular matrix remodeling mechanisms during the early acute phase of natural spinal …
Transcriptional And Morphological Changes During Thyroxine-Induced Metamorphosis Of The Mexican Axolotl And Axolotl-Tiger Salamander Hybrids, Robert Bryce Page
Transcriptional And Morphological Changes During Thyroxine-Induced Metamorphosis Of The Mexican Axolotl And Axolotl-Tiger Salamander Hybrids, Robert Bryce Page
University of Kentucky Doctoral Dissertations
For nearly a century, amphibian metamorphosis has served as an important model of how thyroid hormones regulate vertebrate development. Consequently metamorphosis has been studied in a number of ways including: morphologically, developmentally, ecologically, and from an endocrine perspective. Over the last two decades, much has been learned about the molecular basis of anuran (frog) metamorphosis. However, very little is known about the molecular underpinnings of urodele (salamander) metamorphosis. Using the axolotl and axolotl hybrids as models, I present some of the first studies on the gene expression changes that occur during urodele metamorphosis. In Chapter 1, the motivation for the …