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Pine, Aphids, And Parasitoid Wasps: Patterns Of Cospeciation And Host Switches In A Tri-Trophic System, Amber Bass May 2019

Pine, Aphids, And Parasitoid Wasps: Patterns Of Cospeciation And Host Switches In A Tri-Trophic System, Amber Bass

Electronic Theses and Dissertations

Ecological interactions may drive speciation events, and the processes that drive these speciation events can leave behind patterns in the phylogenies of interacting taxa. These patterns have been studied extensively in herbivores and host plants, as well as parasites and their hosts, but rarely in tri-trophic systems. Here, we examine three closely related groups of interacting taxa, including parasitoid wasps (Pauesia), aphid herbivores (Cinara), and pine trees (Pinus) to determine if the patterns between each interacting taxa indicate that cospeciation or host switches are more dominant. We create phylogenies of Cinara and Pauesia in the southeastern United States using ddRADseq …


Do Per-Capita Impact Or Abundance Dominate The Impact Of An Invader? Interactions Among Neighboring Species In Context-Dependent Competition, Haoyu Li May 2019

Do Per-Capita Impact Or Abundance Dominate The Impact Of An Invader? Interactions Among Neighboring Species In Context-Dependent Competition, Haoyu Li

Electronic Theses and Dissertations

Evaluating relative species competitive strength is a central question in community ecology, with strong implications for invasion ecology. Models assessing invader success consider three components: distribution, abundance and per-capita impact. However, relative strength and interactions among these factors remain unclear when applying to specific invasion scenarios. We hypothesized that performance of native and non-native species will vary as a function of direct and indirect effects at different abundances and scales. We conducted a replacement experiment between two dominant grasses in subtropical grasslands (the native Axonopus fissifolius and the non-native Paspalum notatum) in central Florida, USA. Thirty fenced plots (1 m …


Biogeochemical Effects Of Sea Level Rise-Induced Transitions Within Coastal Wetlands, Havalend Steinmuller May 2019

Biogeochemical Effects Of Sea Level Rise-Induced Transitions Within Coastal Wetlands, Havalend Steinmuller

Electronic Theses and Dissertations

As sea level rise (SLR) affects coastal wetlands, ecosystem responses can include vertical accretion, landward transgression, or submergence. Sea level rise-induced transitions can alter key biogeochemical transformations within wetland soils, impacting the ability of these systems to provide ecosystem services, specifically carbon (C) storage and water quality regulation. Through a series of complementary laboratory and field-based studies, biogeochemical responses to salinity, vegetation shifts, and submergence were investigated. Changes in nutrient dynamics associated with saltwater intrusion were evaluated by artificially adding saline water to different freshwater wetland soil types, indicating that potential exports of critical nutrients (forms of nitrogen, phosphorus, and …


Exploring Multi-Scale Variation Of Fish Community Diversity In A Dynamic Coastal Estuary, Brittany Troast Jan 2019

Exploring Multi-Scale Variation Of Fish Community Diversity In A Dynamic Coastal Estuary, Brittany Troast

Electronic Theses and Dissertations

Examining diversity over multiple spatial and temporal scales affords the opportunity to develop a mechanistic understanding of the factors influencing community diversity dynamics, and how these may shift in a changing world. This thesis first examines multi-decadal fish community diversity metrics across a coastal biogeographic transition zone to quantify changes in species assemblages, assess relationships between fish community diversity and the abiotic environment, and capture potential shifts in the location of a putative biogeographic break. Results of this chapter indicate not only a change in fish community composition, but also a shift in the location of the biogeographic transition zone. …


Assessing The Effects Of Habitat And Manatee Exclusion Devices On Red Drum (Sciaenops Ocellatus) Movement Patterns In Estuarine Impoundments, Steven Baker Jan 2019

Assessing The Effects Of Habitat And Manatee Exclusion Devices On Red Drum (Sciaenops Ocellatus) Movement Patterns In Estuarine Impoundments, Steven Baker

Electronic Theses and Dissertations

The Integrate-Transfer-Launch (ITL) complex impoundments located within the Kennedy Space Center security zone are known spawning sites for red drum (Sciaenops ocellatus). However, recent construction of manatee exclusion bollards around culverts leading into these impoundments may impede movement to and from this critical habitat. The goals of this study were to: 1) utilize passive acoustic telemetry to document patterns of red drum movement and habitat use within and around the ITL impoundments to assess how manatee exclusion bollards and environmental conditions may impact movement of red drum; and 2) explore red drum movement in response to environmental disturbance, specifically a …


Understanding Sediment Biogeochemistry And The Role Of Juvenile Oysters On Recently Restored Eastern Oyster Reefs, Bryan Locher Jan 2019

Understanding Sediment Biogeochemistry And The Role Of Juvenile Oysters On Recently Restored Eastern Oyster Reefs, Bryan Locher

Electronic Theses and Dissertations

In recent decades, goals for the restoration of eastern oyster (Crassostrea virginica) populations along the eastern coast of the United States have shifted from increasing harvestable oyster fisheries to enhancing the range of ecosystem services provided by oyster reefs. By filtering large volumes of water and releasing nutrient-rich feces and pseudofeces, oysters can locally enhance sediment biogeochemical cycling compared to that of unstructured benthic environments. An ongoing restoration program in Mosquito Lagoon, FL was leveraged to assess the immediate impacts ( < 1 year) of restoration on sediment biogeochemical properties of oyster reefs. The first study measured both short-term and long-term pools of carbon, nitrogen and phosphorus on dead, natural and restored reefs periodically over one year. The second study investigated one of the contributions to sediment nutrient pools by comparing feeding and feces/pseudofeces nutrient content of juvenile and older oysters. Results show that inorganic nitrogen and phosphorus pools can change within weeks after restoration and total nutrient pools by 6 months post-restoration. Restored reefs experienced a 136 % increase in ammonium, 78 % increase in total nitrogen, 46 % increase in total phosphorus, and 75 % increase in organic matter concentrations after 12 months of restoration. These nutrient increases were all positively correlated with oyster density, shell length and reef height measured on each reef. When standardized to grams of dry tissue weight, juvenile oysters showed significantly higher rates of chlorophyll-a removal, release of ammonium, and biodeposits with higher concentrations of dissolved organic carbon, nitrite + nitrate, and ammonium. The short-term changes to biogeochemical cycling on eastern oyster reefs within the first year of restoration are important to managers seeking to monitor ecosystem service recovery and overall coastal ecosystem health.


Business In The Estuary, Party In The Sea: Migration Patterns Of Striped Mullet (Mugil Cephalus) Within The Indian River Lagoon Complex, Olivia Myers Jan 2019

Business In The Estuary, Party In The Sea: Migration Patterns Of Striped Mullet (Mugil Cephalus) Within The Indian River Lagoon Complex, Olivia Myers

Electronic Theses and Dissertations

Commercial and recreational environmental enterprises in the Indian River Lagoon (IRL), Florida supply nearly 10,000 jobs and produce $1.6 billion dollars a year in revenue. These waters contain iconic species of sportfish, including red drum, snook, and sea trout, as well as their lower trophic level prey such as snapper and mullet. Striped mullet (Mugil cephalus) are both commercially valuable as well as an indicator species for overall ecosystem health. From September to December, mullet in the IRL undergo an annual migration from their inshore foraging habitats to oceanic spawning sites. However, their actual migratory pathways remain unknown. To address …


Seascape Genetics And Rehabilitation Efficiency In The Florida Manatee, Madison Hall Jan 2019

Seascape Genetics And Rehabilitation Efficiency In The Florida Manatee, Madison Hall

Electronic Theses and Dissertations

The Florida manatee (Trichechus manatus latirostris) was recently downlisted federally from "endangered" to "threatened" despite acknowledgments of remaining threats to long term population persistence. Challenges to future manatee conservation include, but are not limited to, increases in frequency of harmful algal blooms, intensifying anthropogenic disturbance, and loss of warm-water habitat. The goals of this dissertation were 1) to assess threats to the manatee via a comprehensive, long-term (1973-2016), retrospective analysis of the manatee rescue and rehabilitation partnership (MRRP) and 2) to use seascape genetics analysis to examine whether abiotic, biotic, or anthropogenic seascape variables could significantly describe genetic distance patterns …


The Ecology Of Central Florida's Thief Ants (Hymenoptera: Formicidae: Solenopsis), Leo Ohyama Jan 2019

The Ecology Of Central Florida's Thief Ants (Hymenoptera: Formicidae: Solenopsis), Leo Ohyama

Electronic Theses and Dissertations

Thief ants of the genus Solenopsis are a diverse group of ants that are found in ant communities throughout the world. They have long been purported to practice lestobiosis, an interaction between small and larger-bodied ants, where small ants cryptically tunnel into larger-bodied ant nests within the subterranean environment and steal brood or eggs for consumption. Thief ants are extremely small, measuring 1-2 mm in length and many of the species within this group practice a subterranean life history, where they live the entirety of their lives exclusively belowground. Due to these key characteristics, the ecology and natural history of …


Hydrodynamic Limitations And The Effects Of Living Shoreline Stabilization On Mangrove Recruitment Along Florida Coastlines, Christian Pilato Jan 2019

Hydrodynamic Limitations And The Effects Of Living Shoreline Stabilization On Mangrove Recruitment Along Florida Coastlines, Christian Pilato

Electronic Theses and Dissertations

The recruitment success of mangroves is influenced by a variety of factors, including propagule availability, desiccation, herbivory, and hydraulic habitat limitations. Hydrodynamic forces (waves and currents) act as obstacles to mangrove recruitment, restricting the successful colonization of mangrove species. We evaluated the biological and physical limitations to mangrove recruitment through monthly shoreline surveys and lateral pull-tests. Surveys followed mangroves from propagule release through recruitment along the shorelines of De Soto National Memorial (Bradenton, FL), capturing differences in propagule availability and recruitment along natural areas and across differing forms of shoreline stabilization ("living shorelines" and revetments). Propagule densities were highest along …


The Hyperaccumulation Of Zinc In Sunflowers And Its Effect On Disease Resistance, Rayner J. Seavey Jan 2019

The Hyperaccumulation Of Zinc In Sunflowers And Its Effect On Disease Resistance, Rayner J. Seavey

Honors Undergraduate Theses

Sunflowers are considered to be a part of a group of plants known as hyperaccumulators that share the ability to accumulate high amounts of heavy metals in the above ground organs, far in excess of the levels found in other species, often without suffering any phytotoxic effects. Quantifying the effects of zinc accumulation through the lens of the elemental defense hypothesis is essential for uncovering if there is a means to increase herbivore resistance in agricultural settings without the use of external interventions such as pesticides. A greenhouse study was conducted on four widely grown commercial cultivars of sunflower. Each …


Investigation Of Microplastic Accumulation In The Gastrointestinal Tract In Birds Of Prey, Julia Carlin Jan 2019

Investigation Of Microplastic Accumulation In The Gastrointestinal Tract In Birds Of Prey, Julia Carlin

Honors Undergraduate Theses

Plastic pollution is unavoidable in the natural environment. Consequences of plastic ingestion include exposure to environmental pollutants and toxin accumulation, causing endocrine disruption, inflammatory and physiological stress in organisms. Microplastics have been shown to transfer across food webs, however, limited studies have examined microplastic accumulation across terrestrial food webs. Furthermore, few studies have examined plastic pollution in apex predatory animals. A study was conducted to quantify the abundance of plastic pollution in the gastrointestinal tract in birds of prey. Two species were investigated, one which forages in terrestrial habitats and one which forages in aquatic environments including Buteo lineatus (red-shouldered …


Assessing The Impact Of Oyster Reef And Living Shoreline Restoration On Macroinvertebrate Community Assemblages In Mosquito Lagoon, Florida, Adam Searles Jan 2019

Assessing The Impact Of Oyster Reef And Living Shoreline Restoration On Macroinvertebrate Community Assemblages In Mosquito Lagoon, Florida, Adam Searles

Honors Undergraduate Theses

As the world continues to experience substantial rates of habitat loss, habitat restoration has become of prime interest to ecologists worldwide. Restoration has shown to be successful in recovering targeted components of certain ecosystems but it is important to achieve a holistic understanding of the resulting ecological impacts it has on communities. To address this, four oyster reefs and three living shorelines were restored during the summer of 2017. These sites, along with four dead oyster reefs, four living oyster reefs, and three undisturbed (control) living shorelines, were sampled before restoration and regularly post-restoration for one year using lift nets. …


Micromanipulation And Genetic Analysis Of Individual Sperm Cells For Sexual Assault Investigations, Amanda Penn Jan 2019

Micromanipulation And Genetic Analysis Of Individual Sperm Cells For Sexual Assault Investigations, Amanda Penn

Honors Undergraduate Theses

Sexual assault investigations utilize both physical and biological evidence to aid in the investigation. Physical evidence may include fingerprints, hair, fibers, stains, soil, and glass. Biological evidence may include semen, saliva, vaginal secretions, menstrual blood, and skin. Semen, often found in small or trace quantities, is of great importance when trying to identify the perpetrator. From the semen sample, DNA profiles using autosomal short tandem repeats (aSTRs) (gold standard in forensic science) or Y-chromosome short tandem repeats (Y-STRs) can be obtained and can be used to identify a perpetrator through comparison to suspect reference samples or by searching the profile …


Investigating The Role Of Neuronal Aging In Fragile X-Associated Tremor/Ataxia Syndrome, Katlin Marie Hencak Jan 2019

Investigating The Role Of Neuronal Aging In Fragile X-Associated Tremor/Ataxia Syndrome, Katlin Marie Hencak

Honors Undergraduate Theses

Fragile X-associated tremor/ataxia syndrome (FXTAS) is an X-linked late-onset neurodegenerative disorder caused by a noncoding trinucleotide repeat expansion in the FMR1 gene. This gene produces fragile x mental retardation protein (FMRP), an RNA binding protein whose targets are involved in brain development and synaptic plasticity. One of the proposed mechanisms of FXTAS pathogenesis is an RNA gain-of-function in which the repeat expansion causes toxic mRNA that sequesters important proteins in the cell, interfering with their functions. Another suggested method of pathogenesis is through a mutant protein called FMRpolyG. This protein results from repeat-associated non-AUG (RAN) translation, in which the expanded …


Fear And Loathing In The Super Organism: Foraging Strategy Doesn't Change Forager Response In A Landscape Of Fear., Philip Schadegg Jan 2019

Fear And Loathing In The Super Organism: Foraging Strategy Doesn't Change Forager Response In A Landscape Of Fear., Philip Schadegg

Electronic Theses and Dissertations

Understanding how predators impact keystone species, like ants, is very important for our understanding of ecology because of ants' importance in shaping community dynamics and ecosystem functions. In this thesis I present research investigating the role of the ant-specialized spider Anasaitis canosa in influencing the foraging behavior of four ant species (Formica pallidefulva, Odontomachus ruginodis, Pheidole obscurithorax & Solenopsis invicta). Collectively, these four species use foraging strategies exhibited by most ants. I conducted two experiments to quantify the impacts of spider predation on ant prey. The first used forty colonies of four ant species to investigate how A. canosa changed …


Variation In Prospecting Behavior And Drivers Of Post-Fire Habitat Preference Among Juvenile Florida Scrub-Jays, David Sherer Jan 2019

Variation In Prospecting Behavior And Drivers Of Post-Fire Habitat Preference Among Juvenile Florida Scrub-Jays, David Sherer

Electronic Theses and Dissertations

Florida Scrub-Jays (Aphelocoma coerulescens, FLSJ; federally Threatened) are cooperatively breeding birds endemic to Florida and dependent on fire-maintained xeric oak scrub. FLSJs are year-round residents, highly territorial, and rarely disperse far from their natal territory. Lifetime reproductive success is highest among individuals breeding in early-successional habitat, usually less than 9 to 10 years post-fire. However, because scrub burns infrequently such early-successional, high-quality habitat is extremely limited and competition for it as breeding space is likely intense. Because some birds live long enough to experience habitat succession, FLSJs also occupy later-successional overgrown scrub, even though both survival and fecundity decline. Although …


Ribbeting Results: Emergent Infectious Diseases In Wetland Systems, Ariel Horner Jan 2019

Ribbeting Results: Emergent Infectious Diseases In Wetland Systems, Ariel Horner

Electronic Theses and Dissertations

North American amphibians have recently been impacted by two major emerging pathogens, the fungus Batrachochytrium dendrobatidis (Bd) and iridoviruses in the genus Ranavirus (Rv). Environmental, seasonal and host factors may play important roles in disease dynamics, but few studies incorporate these components into their analyses. Here, we investigated the role of environmental, seasonal, genetic and location effects on driving Bd and Rv infection prevalence and severity in a biodiversity hot spot, the southeastern United States. We used quantitative PCR to characterize Bd and Rv dynamics in natural populations of three amphibian species: Notophthalmus perstriatus, Hyla squirella and Pseudacris ornata and …


Capillary Electrophoresis Buffer Optimization For Plant Tissue Analysis, Rebekah Davis Jan 2019

Capillary Electrophoresis Buffer Optimization For Plant Tissue Analysis, Rebekah Davis

Honors Undergraduate Theses

Capillary electrophoresis (CE) is an analytical chemistry approach that allows for the efficient separation by charge of diverse classes of compounds for analysis, including secondary metabolites. The goal of this work was to optimize a buffer system for plant tissue analysis using micellar electrokinetic chromatography (MEKC), and by doing so to understand the role of buffer components in the performance of this form of capillary electrophoresis. In this experiment we implemented a factorial design to optimize buffer composition for separating plant tissue and secondary metabolites. The results of this experiment will be used to optimize a universal buffer for MEKC …


Elucidating The Role Of Oxygen And Biotype In The Environmental Persistence Of Vibrio Cholerae, Amy M. Freiberg Jan 2019

Elucidating The Role Of Oxygen And Biotype In The Environmental Persistence Of Vibrio Cholerae, Amy M. Freiberg

Honors Undergraduate Theses

Vibrio cholerae is a natural inhabitant of aquatic environments and serves as the etiological agent for the severe diarrheal disease, cholera. Cholera epidemics follow a regular seasonal pattern, which account for tens to hundreds of thousands of deaths in a given year. V. choleraenaturally persist between epidemics through entry into a dormant state known as viable but nonculturable (VBNC). Research has shown that V. choleraein this VBNC state experience drastic morphological and metabolic changes, which serve as survival mechanisms until environmental conditions become suitable again. The natural marine ecosystem that V. cholerae inhabitis comprised of a complex …