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Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Articles
The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4-Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zheng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4-Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zheng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang
Articles
The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …