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Assessment Of Arbuscular Mycorrhizal Symbiosis On Invasion Success In Brachypodium Sylvaticum, Caitlin Elyse Lee
Assessment Of Arbuscular Mycorrhizal Symbiosis On Invasion Success In Brachypodium Sylvaticum, Caitlin Elyse Lee
Dissertations and Theses
The effects that mutualistic soil biota have on invasive species success is a growing topic of inquiry. Studies of the interactions between invasive plants and arbuscular mycorrhizal fungi (AMF) have shown changes in AMF community composition, reductions in AMF associations in invasive plants, and changes in native species fitness and competitive outcomes in invasive-shifted AMF communities. These findings support the degraded mutualist hypothesis, where invasive species alter the mutualist community composition, resulting in detrimental associations with the new mutualist community for native species. Here I present two studies that examine various aspects of the arbuscular mycorrhizal fungal (AMF) mutualism in …
Characterization Of Functional Domains Of Cul3, An E3 Ubiquitin Ligase, Using Chimeric Analysis, Jennifer Anne Mitchell
Characterization Of Functional Domains Of Cul3, An E3 Ubiquitin Ligase, Using Chimeric Analysis, Jennifer Anne Mitchell
Dissertations and Theses
Modification of cellular proteins with molecules of ubiquitin is an important process that regulates the activity of cellular proteins. Cullin RING ligases (CRLs) are multi-subunit complexes that act in concert with E2 enzymes to attach molecules of ubiquitin to protein substrates. There are seven CRLs in mammalian cells (Cul1, Cul2, Cul3, Cul4A, Cul4B, Cul5, and Cul7) that are highly homologous in sequence and structure. CRLs possess a highly conserved C- terminal domain that interacts with E2 enzymes, and a more variable N- terminal domain which recruits substrates through distinct substrate adapter molecules. Despite the structural similarity, these CRLs recognize distinct …
The Orientation Of The Pyrococcus Furiosus Transcription Factor Tfb2 In The Transcription Initiation Complex, Arati Bhattarai
The Orientation Of The Pyrococcus Furiosus Transcription Factor Tfb2 In The Transcription Initiation Complex, Arati Bhattarai
Dissertations and Theses
The hyperthermophile archaeon, Pyrococcus furiosus encodes two eukaryotic TFIIB family proteins, TFB1 and TFB2. TFB1 is very similar to TFIIB in terms of sequence homology and function, whereas TFB2 is unusual as it is missing highly conserved sequences in its N-terminal domain that are present in TFIIB and TFB1. Despite this, TFB2 is effective in transcription process, albeit with lower efficiency compared to TFB1. Other archaea also contain multiple TFBs, but unlike Pyrococcus furiosus TFB2, these multiple TFBs have higher sequence homology to each other and have similar transcription efficiencies. Photochemical cross-linking experiments have shown that the B-reader of TFB …
Temperature Dependent Transcription Initiation In Archaea: Interplay Between Transcription Factor B And Promoter Sequence, Ming-Hsiao Wu
Temperature Dependent Transcription Initiation In Archaea: Interplay Between Transcription Factor B And Promoter Sequence, Ming-Hsiao Wu
Dissertations and Theses
In Pyrococcus furiosus (Pfu), a hyperthermophile archaeon, two transcription factor Bs, TFB1 and TFB2 are encoded in the genomic DNA. TFB1 is the primary TFB in Pfu, and is homologous to transcription factor IIB (TFIIB) in eukaryotes. TFB2 is proposed to be a secondary TFB that is compared to TFB1, TFB2 lacks the conserved B-finger / B-reader / B-linker regions which assist RNA polymerase in transcription start site selection and promoter opening functions respectively. P. furiosus, like all Archaea, encodes a single transcription factor E (TFE), that is homologous to the N-terminus of transcription factor II …