Digital Commons Network^™

Metabolic Profiling Reveals Biochemical Pathways Responsible For Eelgrass Response To Elevated Co2 And Temperature, Carmen C. Zayas-Santiago, Albert Rivas-Ubach, Li-Jung Kuo, Nicholas D. Ward, Richard C. Zimmerman

OES Faculty Publications

As CO₂ levels in Earth’s atmosphere and oceans steadily rise, varying organismal responses may produce ecological losers and winners. Increased ocean CO₂ can enhance seagrass productivity and thermal tolerance, providing some compensation for climate warming. However, the metabolic shifts driving the positive response to elevated CO₂ by these important ecosystem engineers remain unknown. We analyzed whole-plant performance and metabolic profiles of two geographically distinct eelgrass (Zostera marina L.) populations in response to CO₂ enrichment. In addition to enhancing overall plant size, growth and survival, CO₂ enrichment increased the abundance of Calvin Cycle and …

Metagenomic Sequencing Identifies Highly Diverse Assemblages Of Dinoflagellate Cysts In Sediments From Ships' Ballast Tanks, Lixia Shang, Zhangxi Hu, Yunyan Deng, Yuyang Liu, Xinyu Zhai, Zhaoyang Chai, Xiaohan Liu, Zifeng Zhan, Fred C. Dobbs, Ying Zhong Tang

OES Faculty Publications

Ships' ballast tanks have long been known as vectors for the introduction of organisms. We applied next-generation sequencing to detect dinoflagellates (mainly as cysts) in 32 ballast tank sediments collected during 2001-2003 from ships entering the Great Lakes or Chesapeake Bay and subsequently archived. Seventy-three dinoflagellates were fully identified to species level by this metagenomic approach and single-cell polymerase chain reaction (PCR)-based sequencing, including 19 toxic species, 36 harmful algal bloom (HAB) forming species, 22 previously unreported as producing cysts, and 55 reported from ballast tank sediments for the first time (including 13 freshwater species), plus 545 operational taxonomic units …

Phytoplankton Plastid Proteomics: Cracking Open Diatoms To Understand Plastid Biochemistry Under Iron Limitation, Skyler J. Nunn, Phoebe Dreux Chappell, Kristofer Gomes, Anasthasia Bonderenko, Bethany D. Jenkins, Brook L. Nunn

OES Faculty Publications

Diatoms, such as Thalassiosira pseudonana, are important oceanic primary producers, as they sequester carbon dioxide (CO₂) out of the atmosphere, die, and precipitate to the ocean floor. In many areas of the world’s oceans, phytoplankton, such as diatoms, are limited in growth by the availability of iron (Fe). Fe is an essential nutrient for phytoplankton, as it is central in the electron transport chain component of photosynthesis. Through this study, we examined if Fe-limitation makes a significant difference in the proteins expressed within the chloroplast, the power source for diatoms. Here, we utilized a new plastid isolation technique specific …

Predicting Effects Of Ocean Warming, Acidification, And Water Quality On Chesapeake Region Eelgrass, Richard C. Zimmerman, Victoria J. Hill, Charles L. Gallegos

OES Faculty Publications

Although environmental requirements of seagrasses have been studied for years, reliable metrics for predicting their response to current or future conditions remain elusive. Eelgrass (Zostera marina L.) populations of the Chesapeake region lie near the southern limit of their range in the Western North Atlantic, exposing them to increasing thermal stress as the climate warms. However, CO₂ stimulated photosynthesis may offset some of the negative effects of temperature stress. The combined effects of temperature, CO₂, and light availability controlled by water quality and epiphytes were explored using GrassLight, a bio-optical model that provided a predictive …

Reproductive Phase Determination In Male Meagre (Argyrosomus Regius, Sciaenidae): Testis Development And Histologic Corroboration Of A Gross Anatomical Scale, Nuno Prista, Leonel Gordo, José Lino Costa, Maria José Costa, Cynthia Jones

OES Faculty Publications

Reproductive stage determination of male gonads has received sparse attention in fish biology literature with few studies detailing the building of gross anatomical- and histologic scales. The meagre (Argyrosomus regius) is one of the world's largest sciaenids and supports a significant regional fishery in European and North African waters whose reproductive patterns are yet to be fully investigated. In the present study, we derive a macroscopic grading system for meagre testis using semi-quantitative graphs that feature the testis variability along the species size range and time of the year. We then describe the histological stages and reproductive phases of male …

Synergistic Effects Of Iron And Temperature On Antarctic Phytoplankton And Microzooplankton Assemblages, J. M. Rose, Y. Feng, G. R. Ditullio, R. B. Dunbar, C. E. Hare, P. A. Lee, M. Lohan, M. Long, W. O. Smith Jr., B. Sohst, S. Tozzi, Y. Zhang, D. A. Hutchins

OES Faculty Publications

Iron availability and temperature are important limiting factors for the biota in many areas of the world ocean, and both have been predicted to change in future climate scenarios. However, the impacts of combined changes in these two key factors on microbial trophic dynamics and nutrient cycling are unknown. We examined the relative effects of iron addition (+1 nM) and increased temperature (+4° C) on plankton assemblages of the Ross Sea, Antarctica, a region characterized by annual algal blooms and an active microbial community. Increased iron and temperature individually had consistently significant but relatively minor positive effects on total phytoplankton …

Geobiology: Evidence For Early Life On Earth And The Search For Life On Other Planets, Sherry L. Cady, Nora Noffke

OES Faculty Publications

Extensive research efforts in the subdisciplinary field of geobiology have focused on the interactions between Earth and life through time. As a consequence, gaps in our knowledge of Earth’s history are closing, and the search for life beyond Earth is expanding. A few examples of geobiology studies designed to advance our understanding of life on early Earth and to improve the chances of finding life on other planets are provided to highlight recent developments and research areas that are on the verge of new discoveries.

Potential Export Of Unattached Benthic Macroalgae To The Deep Sea Through Wind Driven Langmuir Circulation, H. M. Dierssen, Richard C. Zimmerman, Lisa A. Drake, David J. Burdige

OES Faculty Publications

Carbon export to the deep sea is conventionally attributed to the sinking of open ocean phytoplankton. Here, we report a Langmuir supercell event driven by high winds across the shallow Great Bahama Bank that organized benthic non-attached macroalgae, Colpomenia sp., into visible windrows on the seafloor. Ocean color satellite imagery obtained before and after the windrows revealed a 588 km² patch that rapidly shifted from highly productive macroalgae to bare sand. We assess a number of possible fates for this macroalgae and contend that this event potentially transported negatively buoyant macroalgae to the deep Tongue of the Ocean …

Response Of Eelgrass Zostera Marina To Co2 Enrichment: Possible Impacts Of Climate Change And Potential For Remediation Of Coastal Habitats, Sherry L. Palacios, Richard C. Zimmerman

OES Faculty Publications

Projected increases in dissolved aqueous concentrations of carbon dioxide [CO_2(aq)] may have significant impacts on photosynthesis Of CO₂-limited organisms such as seagrasses. Short-term CO_2(aq) enrichment increases photosynthetic rates and reduces light requirements for growth and survival of individual eelgrass Zostera marina L. shoots growing in the laboratory under artificial light regimes for at least 45 d. This study examined the effects of long-term CO_2(aq) enrichment on the performance of eelgrass growing under natural light-replete (33% surface irradiance) and light-limited (5% surface irradiance) conditions for a period of 1 yr. Eelgrass shoots were grown at …

Length-At-Age In Juvenile Patagonian Toothfish (Dissostichus Eleginoides), J. Ashford, C. Jones, S. Bobko, I. Everson

OES Faculty Publications

We used the otoliths from a sample of Dissostichus eleginoides pre-recruits, whose length density was distinctly polymodal, to see whether ages estimated by reading otoliths were congruent with the length modes observed. Length densities by age were compared graphically with the overall length density observed. Additionally, ages were predicted for each fish based on length, and compared with ages estimated from reading otoliths in a goodness-of-fit test. The majority of the otoliths (83.6%) were estimated to be from fish 1+ or 2+ years old, with mean total lengths of 32.5 cm and 41.3 cm respectively. No difference was found between …

Yield-Per-Recruit Analysis For Black Drum, Pogonias Cromis, Along The East Coast Of The United States And Management Strategies For Chesapeake Bay, Cynthia M. Jones, Brian K. Wells

OES Faculty Publications

Black drum, Pogonias cromis along the U.S. East Coast is subject to commercial and recreational harvest. However, prior to this study no modeling had been undertaken to examine the potential for overfishing in the Chesapeake Bay region. We present evidence from yield-per-recruit models that growth overfishing of black drum is unlikely under current fishing practices in this region. Particular attention was given to fishing practices in the Chesapeake Bay region where old, large fish predominate in the commercial and recreational catches (mean age=26 years: mean total length=108.4 cm; mean weight 22.1 kg). Yield-per-recruit model results showed that growth overfishing was …

Limitation Of Algal Growth By Iron Deficiency In The Australian Subantarctic Region, Peter N. Sedwick, Giacomo R. Ditullio, David A. Hutchins, Philip W. Boyd, F. Brian Griffiths, A. Clive Crossley, Thomas W. Trull, Bernard Queguiner

OES Faculty Publications

In March 1998 we measured iron in the upper water column and conducted iron- and nutrient-enrichment bottle-incubation experiments in the open-ocean Subantarctic region southwest of Tasmania, Australia. In the Subtropical Convergence Zone (∼42°S, 142°E), silicic acid concentrations were low (< 1.5μM) in the upper water column, whereas pronounced vertical gradients in dissolved iron concentration (0.12-0.84 nM) were observed., presumably reflecting the interleaving of Subtropical and Subantarctic waters, and mineral aerosol input. Results of a bottle-incubation experiment performed at this location indicate that phytoplankton growth rates were limited by iron deficiency within the iron-poor layer of the euphotic zone. In the Subantarctic water mass (∼46.8°S, 142°E), low concentrations of dissolved iron (0.05-0.11nM) and silicic acid (< 1μM) were measured throughout the upper water column, and our experimental results indicate that algal growth was limited by iron deficiency. These observations suggest that availability of dissolved iron is a primary factor limiting phytoplankton growth over much of the Subantarctic Southern Ocean in the late summer and autumn.

Age, Growth, And Mortality Of Black Drum, Pogonias Cromis, In The Chesapeake Bay Region, Cynthia M. Jones, Brian Wells

OES Faculty Publications

We used otolith ageing to describe the population dynamics of black drum, Pogonias cromis, collected over a three-year period from the Chesapeake Bay region's commercial and recreational fisheries. Black drum average age, total length, and weight were 26 years, 109.5 cm, and 22.1 kg respectively. The oldest fish was 59 years and fish older than 50 years were present in the catch from 1990 to 1992. Growth in length slowed by age 20, whereas growth in weight did not slow until age 45. A von Bertalanffy growth function was fitted to our data (L(infinity) = 117.3 cm, …

Modeling Daily Production Of Aquatic Macrophytes From Irradiance Measurements: A Comparative Analysis, Richard C. Zimmerman, Alejandro C. Pasini, Randall S. Alberte

OES Faculty Publications

The importance of submerged aquatic macrophytes to coastal ecosystems has generated a need for knowledge of minimum light levels that will support the maintenance and restoration of healthy populations. Our goals were (1) to evaluate the sensitivity to natural, non-sinusoidal fluctuations in irradiance I of analytical integration techniques for calculating daily carbon gain, (2) to evaluate the H_sat (the daily period of I-saturated photosynthesis) model of daily production relative to models based on instantaneous photosynthesis vs irradiance (P vs I) and (3) to provide some guidance for the temporal density of irradiance data required for accurate estimation of …

A Comparison Of No And N20 Production By The Autophic Nitrifier Nitrosomonas Europaea And The Heterotrophic Nitrifier Alcaligenes Faecalis, Iris C. Anderson, Mark Poth, Julie Homstead, David J. Burdige

OES Faculty Publications

Soil microorganisms are important sources of the nitrogen trace gases NO and N₂O for the atmosphere. Present evidence suggests that autotrophic nitrifiers such as Nitrosomonas europaea are the primary producers of NO and N₂O in aerobic soils, whereas denitrifiers such as Pseudomonas spp. or Alcaligenes spp. are responsible for most of the NO and N₂O emissions from anaerobic soils. It has been shown that Alcaligenes faecalis, a bacterium common in both soil and water, is capable of concomitant heterotrophic nitrification and denitrification. This study was undertaken to determine whether heterotrophic nitrification might be …

Development Of Pelagic Larvae And Postlarva Of Squilla Empusa (Crustacea, Stomatopoda), With An Assessment Of Larval Characters Within The Squillidae, Steven G. Morgan, Anthony J. Provenzano Jr.

OES Faculty Publications

Larvae of the predatory crustacean Squilla empusa were collected from the plankton in Chesapeake Bay and reared in the laboratory to permit description of the pelagic stages before the postlarval stage.

Characters such as rostral length and spinulation, carapace spinulation, relative size of telson, overall body size, and appearance probably are of more value for specific than for generic identification. The presence or absence of teeth on the dactylus of the second maxilliped, the presence or absence of a spine on the basis of the second maxilliped, and the number of epipods may be useful characters in determining generic alliances …