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A Comparison Of Electronic And Traditional Cigarette Butt Leachate On The Development Of Xenopus Laevis Embryos, Tatiana Tatum Parker, James Rayburn
A Comparison Of Electronic And Traditional Cigarette Butt Leachate On The Development Of Xenopus Laevis Embryos, Tatiana Tatum Parker, James Rayburn
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Potential developmental toxicities of three different cigarette butt leachates were evaluated using the frog embryo teratogenesis assay–Xenopus (FETAX). Xenopus laevis embryos were exposed to regular cigarette butt (RCB), menthol (MCB) and electronic (ECB) in concentrations ranging from 0 to 4 butts/l for RCB and MCB and 0–10 butts/l for ECB. The embryos were from stage 8 to 11 and were exposed for a 96-h period in static renewal test conditions. Median lethal concentration (LC50), malformation (EC50), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. Results from these studies suggest that each tested leachate is …