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Matrices (Re)Loaded: Durability, Viability, And Fermentative Capacity Of Yeast Encapsulated In Beads Of Different Composition During Long-Term Fed-Batch Culture, Jordan Gulli, Peter Yunker, Frank Rosenzweig

Biological Sciences Faculty Publications

Encapsulated microbes have been used for decades to produce commodities ranging from methyl ketone to beer. Encapsulated cells undergo limited replication, which enables them to more efficiently convert substrate to product than planktonic cells and which contributes to their stress resistance. To determine how encapsulated yeast supports long-term, repeated fed-batch ethanologenic fermentation, and whether different matrices influence that process, fermentation and indicators of matrix durability and cell viability were monitored in high-dextrose, fed-batch culture over 7 weeks. At most timepoints, ethanol yield (g/g) in encapsulated cultures exceeded that in planktonic cultures. And frequently, ethanol yield differed among the four matrices …