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Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa Jan 2018

Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa

Electronic Theses and Dissertations

Root provides plant water, nutrients and anchorage from soil. Most our knowledge of molecular mechanisms of root development is from the dicot model plant Arabidopsis, but very few studies have done in monocot crop systems like rice, maize, and wheat. We are studying very short root (VSR) phenotype in wheat, and lack of a sequenced reference genome in wheat prompted us to sequence and assemble the root transcriptome of the reference cultivar Chinese Spring (CS). A root transcriptome was assembled from the sequenced reads generated from root tip and the mature root tissues of CS. Approximately 169 million reads were …


Protein Kinase Activity Toward Taabf1 In Imbibing Grains, Taylor P. Enrico Jan 2017

Protein Kinase Activity Toward Taabf1 In Imbibing Grains, Taylor P. Enrico

Honors Theses

The hormones gibberellin and abscisic acid are essential for plant responses to changing environmental conditions, and can send opposing signals. In wheat, the transcription factor TaABF1 plays an important role at the intersection of a gibberellin-induced/abscisic acid-suppressed pathway. When gibberellin dominates, the GA-induced gene, Amy32b, is transcribed. When abscisic acid is dominant, TaABF1 is active and it downregulates GA-induction of Amy32b, while promoting ABA-induced transcription of the gene HVA1. The activity of TaABF1 is thought to be regulated by post-translational phosphorylation at key serine residues. In this study, to determine TaABF1 phosphorylation by wheat kinases, we purified recombinant …


Analysis Of The Effects Of Hypogeal And Epigeal Emergence On Seedling Competition In Legumes, Keith E. King May 2003

Analysis Of The Effects Of Hypogeal And Epigeal Emergence On Seedling Competition In Legumes, Keith E. King

McCabe Thesis Collection

Seedling emergence is either hypogeal or epigeal. In hypogeal emergence the cotyledons remain below the soil surface during seedling development, while in epigeal emergence the cotyledons extend above the soil surface due to elongation of the hypocotyl. Here we examine the effect of seed size, and emergence type, on seedling growth. Six legumes with variation in seed size and either epigeal or hypogeal emergence were grown under dark and light conditions. A competition study was also conducted where a hypogeal and epigeal legume were grown in combination with wheat and ryegrass. Legumes expressing epigeal emergence were cowpea (Vigna unguiculata), mung …


Some Physical And Chemical Properties Of Wheat Starch In Relationship To The Maturity Of The Wheat, Claude W. Bice Jul 1943

Some Physical And Chemical Properties Of Wheat Starch In Relationship To The Maturity Of The Wheat, Claude W. Bice

Master's Theses

Wheat is one of the most valuable of economic plants and is grown extensively throughout the world. Because of the unique chemical and physical properties of its gluten, wheat is superior to all other cereals for bread making. Its high starch content provides a tremendous source of energy as a food, as well as filling certain industrial starch needs, especially in textile manufacturing . The present study on properties of immature and mature wheat starches should be of inestimable worth for evaluation of damaged wheats for starch production, as well as for yielding fundamental information which may facilitate the use …