Digital Commons Network^™

Characterization Of Neutrophil Extracellular Traps In Naked Mole-Rats: A Step Towards Cancer Resistance, Thomas Abraham Smith

Undergraduate Honors Capstone Projects

Cancer is the second leading cause of death in the United States, exceeded only by heart disease. One of every five deaths in the United States is due to cancer. A growing area of research involves the analysis of cancer resistant traits in other species to understand their biological mechanisms and eventually make translations to human cancer research and clinical treatment. Because of their remarkable cancer resistance, the naked mole-rat (NMR) is a prime subject for this research, and various studies have already suggested that the immune mechanisms of the NMR might be harnessed for human cancer therapies^1-4,7. …

Protein Nutrition And Immunity In Male Bumblebees (Bombus Impatiens), Carson Stoker

Undergraduate Honors Capstone Projects

Male bumble bees play a vital but understudied role in their colonies. They are essential to colony reproduction and success, despite not emerging until the end of the colony's life cycle. Even so, the biology of male bumble bees is not well understood, which leaves our understanding of colony health incomplete. This knowledge gap is important because bumblebee populations are declining, which threatens the ecosystems and industries which rely on them for pollination. This study aimed to understand how two understudied factors of bumble bee health—diet and immunity—are related in males. Pollen, a bee’s main source of protein, has been …

Effect Of Basal Diet And Black Raspberry Supplementation On Gene Biomarkers Of "Leaky Gut" In A Mouse Model Of Colitis-Associated Colorectal Cancer, Eliza Catherine Stewart

Undergraduate Honors Capstone Projects

Colorectal cancer is currently the second leading cause of cancer death in the world. The risk of developing colorectal cancer increases significantly in individuals who suffer from colitis, which is inflammation of the colon lining, seen in Irritable Bowel Disease. The term “leaky gut” is often used to describe increased intestinal permeability and is closely related to colitis. Leaky gut is associated with dysbiosis of the gut microbiota, which can occur through diet. This dysbiosis leads to prolonged inflammation and affects genes that encode for proteins involved in the tight junctions between cells. Compromised intestinal integrity allows for translocation of …

Standardization Of A Protocol To Propagate Gateway Vectors, Kayla Suisse

Undergraduate Honors Capstone Projects

Cloning is an important technique in functional genomics, particularly genetics and molecular biology. Cloned genes can be used to facilitate protein expression; this is an essential step in understanding gene function. In traditional cloning, the gene of interest is amplified and tagged with a restriction enzyme sequence and ligated to the digested open vector. However, the GATEWAY™ Cloning Technology provides a speedy and more efficient route to insert the gene of interest in the desired vector, known as Destination vectors or Expression vectors, using site-specific recombination sites and recombinase enzymes.¹ Once DNA segments from the gene of interest are …

Evolution Of Floral Microbes And The Resulting Effects On Pollinator Preference, Hailey Hatch

Undergraduate Honors Capstone Projects

Floral microbes are an overlooked aspect of the extended floral phenotype. Through altering floral nectar chemistry, they can mediate interactions between flowers, pollinators, and other floral microbes, with significant implications for plant and pollinator health. Interactions between floral microbes and pollinators are critically important to understand, as pollinators provide important ecosystem services in both natural and agriculture systems. Here, I explored how floral nectar traits affected both evolution and competition within the floral yeast Metschnikowia reukaufii, the floral bacterium Bacillus subtilis, and other microbes isolated from Brassica rapa nectar, an important plant model system and oilseed crop. To …

Effects Of Sex And Autism On Oxytocin Receptors In The Substantia Nigra Of The Human Brain, Kip Dooley

Undergraduate Honors Capstone Projects

Oxytocin, a hormone present in the mammalian brain, has been shown to be a vital component of social function in animals and may have a role in the social deficits associated with Autism Spectrum Disorder in humans. Based on previous studies from our lab, there are oxytocin receptors in the human substantia nigra, a basal ganglia structure in the midbrain that is important in both movement and reward pathways. The substantia nigra contains two subsections that are defined by the neurotransmitters they contain: the pars compacta, which is dopaminergic, and the pars reticulata, which is GABAergic. By localizing oxytocin receptors …

Autism & Autoimmunity, Clarissa Nelson

Undergraduate Honors Capstone Projects

My research was for the Honors Capstone, and consisted of familiarizing myself with the vast amount of research in the fields of autism and autoimmunity and how the two may be connected, then going on to design a new project to help acquire more information where these two fields overlap. The official abstract I have written for this new project goes as follows: Our proposed research project is focused primarily in the fields of autism and autoimmunity, and hopes to uncover more evidence that these two fields are related. We believe that autism is an autoimmune disease, and our project …

Emergent Seedling Spatial Patterns Following Insect Seed Predation In A Simulated Population, Justin Tirrell

Undergraduate Honors Capstone Projects

Tropical vertebrate species have faced increasing pressures from hunters, causing many to become locally extinct. I used an agent-based model (NEDD) to investigate the influence of dispersal and insect seed predation on seedling survival. Statistical dispersal kernels were used to simulate the dispersal of seeds. The NEDD model generates survival and spatial data from parameter sets, which were chosen based on a Latin-Hypercube experimental design. Spatial point analysis was performed on the output data to identify trends in spatial clustering patterns as the parameter space was changed. The results of this investigation suggest that there is a positive association between …

Intra- And Interspecific Secondary Metabolite Variation Between Fruit And Leaf Tissues In The Hyperdiverse Psychotria Genus, Cole A. Carlson

Undergraduate Honors Capstone Projects

Secondary metabolites are chemical compounds that are considered to mediate a variety of plant interactions with their environment and are not involved in basic metabolism. Recently, there has been an interest in understanding the function and allocation of these metabolites in fruit tissues. In contrast to leaves, the chemistry in fruit tissue mediates exclusive interactions with seed dispersers that directly affect plant fitness and are under different evolutionary selective pressures. Only a few studies outline the patterns of chemistry between fruit and leaf tissues. This study aims to understand how secondary metabolites in two species of the hyperdiverse congeneric genus …

Exposure Of Phlebotomus Argentipes To Alpha-Cypermethrin, Permethrin, And Ddt Using Cdc Bottle Bioassays To Assess Insecticide Susceptibility, Jacob Rex Andersen

Undergraduate Honors Capstone Projects

Background: Insecticide resistance for sand flies is a concern since sand flies are vectors for Leishmania spp. parasites which cause leishmaniasis affecting millions of people each year. The CDC bottle bioassay is used to assess resistance by comparing known insecticide diagnostic doses and diagnostic times from an insecticide-susceptible population. The objective of this study was to determine diagnostic doses and diagnostic times for α-cypermethrin and the lethal dose for 50% and 90% mortality for α-cypermethrin, permethrin, and DDT for Phlebotomus argentipes.

Methods: The CDC bottle bioassays were performed in 1,000 mL glass bottles with 15-25 sand flies from a laboratory …

The Response Of Male Bumblebees To A Putative Queen Pheromone, Xavier K. Haemmerle

Undergraduate Honors Capstone Projects

Queen pheromones are chemical signals produced by the dominant reproductive female in many species of eusocial insects. These pheromones are vital for maintaining a reproductive division of labor. Two evolutionary scenarios may describe the origin of queen pheromones. Sensory exploitation describes a scenario where the pheromone is produced to take advantage of a preexisting sensory bias in a population. An alternative scenario is that the recipient of the pheromone has an adapted response to a preexisting chemical signal. There is a growing body of evidence that cuticular hydrocarbons that act as queen pheromones are co-opted from ancient fertility signals that …

Effects Of Reproductive Maturity On Neuroplasticity In Male Bumble Bees (Bombus Impatiens), Karlee Eck

Undergraduate Honors Capstone Projects

Within the span of an insect's lifetime, new synaptic connections between neurons are made in response to age and experience. This neuroplasticity leads to anatomical changes within individual brain regions that represent investment in different brain functions. The neuroplasticity of female bumble bees has been well studied, but almost nothing is known about how the male bee brain responds to life experiences. Due to the complex sensory requirements of mate finding and copulation for males, neuroplasticity is likely to be an important component of reproduction. These sensory requirements include olfactory and optic inputs, which are processed in the calyces of …

Evaluating The Potential Of Repurposing Commercially Available Drugs For The Treatment Of Viral Infections, Brennan Connor Mcewan

Undergraduate Honors Capstone Projects

Viral infections that are often overlooked as common seasonal illnesses such as influenza can rapidly become a public threat. They threaten society as new, more dangerous strains of these common viruses emerge and as strains develop resistance to current vaccines and antiviral treatments (Kochanek, Murphy, Xu, & Tejada-Vera, 2014). To combat this, the development of antiviral treatments with novel mechanisms of action is essential. Repurposing drugs instead of developing new drugs can save years of development time and hundreds of millions of dollars (DiMasi, Hansen, & Grabowski, 2003). To support the effort to discover drugs with unique mechanisms of action, …

Transcriptomic Analysis Of Honey Bees Exposed To Organosilicone, Matthew Brent Thompson

Undergraduate Honors Capstone Projects

Honey bees play an important role in agriculture and the decline of honey bee populations worldwide has generated concern. While the application of pesticides in agricultural settings is often implicated in the deterioration of honey bee population health, pesticide applications contain more than just pesticides; they also contain adjuvants that may have detrimental effects to bee health. One known effect of one type of adjuvant is the suppression of immunity-related genes and consequent increase of viral load in larvae. We investigate the effects of one class of adjuvant, organosilicone surfactants (OSS), on adult honey bee health. In a laboratory based …

Evaluation Of Antiviral Agents In Two Mouse Models Of Rna Virus Infections, John Hyrum Mcclatchy

Undergraduate Honors Capstone Projects

The first model we are evaluating is human influenza virus in mice. Influenza virus is a negative sense RNA virus, which the CDC estimates causes between 9.2 million and 35.6 million illnesses each year in the United States.^[1] In the current influenza season, H3N2 viruses have been the predominant subclade of influenza A virus in circulation.^[2] Development of a mouse model for influenza H3N2 virus infection has been difficult. In these studies, we endeavored to mouse-adapt influenza A/Hong Kong/4801/2014 H3N2 virus by serial passaging in AG129 mice. This virus strain was chosen as it overlaps in serotype with …

The Effect Of Gender And Socioeconomic Status On Concussion Reporting Behavior Among Ncaa Student-Athletes, Joshua Anthony Hansen

Undergraduate Honors Capstone Projects

Concussions are traumatic brain injuries that result from "brain shaking" that can occur during any situation that transmits force to the head. Concussions are defined as a clinical syndrome characterized by immediate and transient post-traumatic impairment of neural functions which lead to a complex grouping of both psychological and physiological symptoms (McCrory, et al., 2013). As knowledge of the long-term implications of these injuries grows, concussions are becoming more of a major health concern worldwide. One subset of concussion classifications, sports-related concussions, is receiving an increasing amount of attention from both scientists and health-care practitioners. It is estimated that more …

Maternal Vitamin D Deficiency And Early Childhood Health Outcomes Including Autism Development, Kelsey L. Girardelli

Undergraduate Honors Capstone Projects

Many studies have shown that vitamin D deficiency during pregnancy is associated with a variety of adverse maternal and pediatric outcomes. Disease outcomes that have been observed in pregnant women who are vitamin D deficient include increased risk of C-section, preeclampsia, bacterial vaginosis, and gestational diabetes. In children born to deficient mothers, increased rates of childhood asthma, type 1 diabetes, low birthweight, and autism spectrum disorder (ASD) have been observed. Although there is much evidence to support these correlations, much is yet to be understood regarding the etiology of these outcomes. This paper specifically examines the relationships between risk factors …

Cache Valley Resident Exposure To Pm2.5, Kristina Krepinski

Undergraduate Honors Capstone Projects

Air pollution poses one of the largest environmental risks to human health, and greatly contributes to increased mortality within populations. Of the different types of pollutants, fine particulate matter (PM_2.5) has the most adverse health effects. Long-term exposure to PM_2.5 is known to have serious health outcomes; however, evidence has indicated that even short-term exposure to moderate concentrations of PM_2.5 is detrimental to human health. While PM_2.5 does contribute to various respiratory conditions by affecting lung function, it also significantly affects the cardiovascular system. Elevated PM_2.5 exposure increases risk for cardiovascular disease, congestive heart …

Dietary Supplementation With Tart Cherries For Prevention Of Inflammation-Associated Colorectal Cancer In Mice, Ashlie Hunter

Undergraduate Honors Capstone Projects

The cherry fruit is a nutrient-dense food with comparatively low caloric content and significant amounts of key nutrients and bioactive food chemicals. Much of the health benefit of cherries is attributed to their high amounts of anthocyanins, which have anti-oxidant and anticancer properties that contribute to changes in cell signaling pathways involved in inflammation, carcinogenesis and angiogenesis. In this project, we aimed to determine whether dietary supplementation with tart cherries prevents colon tumor development in mice consuming a Western diet compared to a prudent diet. Previously, our research team developed the Total Western Diet (TWD) for mice to emulate typical …

The Role Of Orexin Receptors In Diurnal Variations In Learning And Memory, Jacob Christian Blotter

Undergraduate Honors Capstone Projects

The brain's ability to learn and remember is a topic of extensive debate and research. Mammals share many similarities, including the way in which information from the outside world is processed and stored. Mammalian circadian rhythms have long been thought to be essentially involved with these processes. Orexin, a neurotransmitter in the brain, plays a crucial role in arousal and circadian rhythm. This study is designed to explore the brain's ability to process and store information at different times of the circadian period, and to explore the role of orexin by comparing the performance of normal (wild-type) mice and abnormal …

Developing A Portable System For Measuring Human Motor Learning, Karen Elizabeth Tew

Undergraduate Honors Capstone Projects

Point-to-point reaching is a commonly used paradigm in the field of human motor control. By studying how people move their arms from one location in space to another, researchers have gained insight into how the central nervous system controls and learns skilled movement. Many experimental methods that are designed to study reaching are not portable. This makes it difficult for researchers to access certain clinical populations with limited mobility or motor dysfunction. We have addressed this issue by developing a point-to-point reaching system that can capture key movement variables (e.g. speed and accuracy) yet is portable and inexpensive. We have …

Group Housing And Social Stress In Side-Blotched Lizards (Uta Stansburiana), Marilize Van Der Walt

Undergraduate Honors Capstone Projects

Stress in regards to an animal's social housing environment is well studied in mammals; however there are few studies examining this in reptiles and the results are thus far unclear. For example, behavioral evidence shows adverse effects from individual housing in juvenile chameleons, however physiological measures in whiptail lizards show adverse effects from group housing. Because some reptiles appear to be affected negatively by their social housing environment while others are impacted positively, if we can discover the reason for these differences, we will be able to implement the most effective housing regimes for reptiles. In this study, we tested …

Sources Of Uncertainty In Stream Nutrient Sampling Below A Point Source, Alexandria Hayden Campbell

Undergraduate Honors Capstone Projects

The goal of this study was to determine what aspects of sampling and sample storage could lead to uncertainty when taking samples in a stream below a point source. Sources of uncertainty studied were the locations where the samples were taken to assess if nutrients were adequately mixed within a cross-section, different filtration techniques, dilution errors, analytical uncertainty, and freezing time. Bootstrapping analyses were used to determine whether mixing and dilution errors led to uncertainty, while one-way ANOVAs were used to evaluate filtration techniques and storage time. Sample spikes to determine percent recovery of nutrients and repeat sample analyses are …

The Effects Of Zno Nanoparticles On Egg, Larva, And Adult Rough-Skinned Newts (Taricha Granulosa), Austin Reid Spence

Undergraduate Honors Capstone Projects

The objective of this study was to examine the effects of zinc oxide (ZnO) nanoparticles on egg, larva, and adult rough-skinned newts, Taricha granulosa. To date, little research has been done to investigate the potentially detrimental effects of nanoparticles on amphibians, especially salamanders and newts (caudates). Chronic toxicity was tested on eggs and larvae, and acute toxicity was tested on eggs, larvae, and adults. For eggs, chronic exposure to ZnO nanoparticles caused higher mortality at 10.0 and 100.0 mg/L compared to 0.0, 0.1, and 1.0 mg/L. When given an acute exposure (24h) to nanoparticles at a late incubation stage, …

Investigating The Pathogenecity Of Chl1 Leu17phe Polymorphism In Schizophrenia, Brooke Nichole Hansen

Undergraduate Honors Capstone Projects

Schizophrenia is a devastating brain disorder that affects a surprising 1% of the world's population. Despite this prevalence, little is known about the molecular aspects of this disorder making it both difficult to diagnose and treat. Several studies have identified the CHL1 gene (Close Homolog of L1), sometimes referred to as CALL, as a risk gene for schizophrenia. CHL1, a neural cell adhesion molecule, has major roles in cell migration, and the development of dendritic and axonal projections. Therefore any deficiency in CHL1 may result in brain defects similar to those identified in schizophrenic populations. Moreover, in genetically engineered mice, …

Increasing Vaccine Accessibility Through Cost Alternative Manufacturing And Elimination Of The Cold Chain, Jorgen Madsen

Undergraduate Honors Capstone Projects

Millions of people die each year from infectious diseases. This is partly due to the difficulty of transporting temperature dependent vaccines through what is called the cold chain in developing countries. I hypothesize that we can increase vaccine accessibility by finding cost effective alternatives to vaccine production and by eliminating the cold chain through vaccine stabilizers. The gold standard in purification of influenza virus is by means of ultracentrifugation. Although effective, this process is very expensive and thus impractical for developing countries. I hypothesize that column chromatography can be a cost efficient alternative that is as effective as ultracentrifugation. The …

Evaluation Of Pro-Inflammatory Biomarkers As Potential Early Indicators Of Acute Respiratory Distress Syndrome (Ards), Makda Solomon Gebre

Undergraduate Honors Capstone Projects

Infections with the 2009 pandemic influenza A(HlNl) virus often leads to acute respiratory distress syndrome (ARDS) in high-risk patients. In these patients, ARDS has a mortality rate of 40-50 % [1]. Patients may die before any intervention is possible since there are few if any early biomarkers that are indicative of ARDS and can be used for diagnosis. In a search for biomarkers that signal early disease progression in mice, multiple acute phase proteins involved with inflammatory responses to infectious stimuli were proposed. Three biomarkers were evaluated for this project. These included C-reactive protein (CRP), Serum Amyloid A (SAA) and …

An Evaluation Of An Auditory Neurophysiological Model, Alysha Nicole Waters

Undergraduate Honors Capstone Projects

Individuals with normal hearing are adept at understanding speech in the presence of noise, such as other speakers or environmental sounds. In contrast, individuals with hearing loss struggle to understand speech in the same adverse conditions. Neural processing in the inferior colliculus (IC) of the brainstem appears to contribute to the ability to separate simultaneous competing sounds. A computational model developed in the Sinex lab reproduces the responses of IC neurons to complex sound mixtures. It seems likely that the model can be applied to improve the processing of speech in noise. The computational model's effectiveness at improving the processing …

Spectrum Noise Exposures - Analysis And Control Implementation, Kyle Lynn Naylor

Undergraduate Honors Capstone Projects

The Dee Glen Smith spectrum at Utah State University has a reputation of having one of the loudest student sections in the nation. For years, students have taken pride in creating a home court advantage by creating an extremely rowdy and loud atmosphere. Many would agree that this is what makes USU basketball so special.

Elevated noise levels, however, have the potential of creating a health hazard to both employees and spectators. Elevated noise levels can cause negative acute effects such as headache, dizziness, nausea, and tinnitus (ringing in the ears). The most likely acute affects that students, employees, and …

Modulation Of Neuronal R-Type Ca2+ Channels (Cav2.3) By G Protein-Coupled Receptors, Lauren S. Page

Undergraduate Honors Capstone Projects

Neuronal R-type Ca²⁺ channels (Ca_v2.3) are expressed at high concentrations within the cortex, hippocampus and striatal regions of the brain, where they participate in neuronal excitability and synaptic signaling. This pattern of expression may signify a connection between Ca_v2.3 channel function and neurological disorders such as Parkinson's Disease (PD) and Huntington's Disease (HD). Both of these disorders are caused by inadequate secretion of the neurotransmitter dopamine within the striatum. The first step in characterizing the potential importance of Ca_v2.3 in PD and HD is to examine its modulation by G-protein-coupled dopamine receptors. Specifically, …