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Fertility Effects Of Genes Causing Reproductive Isolation Inferred From Three-Way Hybrids In Phacelia, Helen Zhujun Hou
Fertility Effects Of Genes Causing Reproductive Isolation Inferred From Three-Way Hybrids In Phacelia, Helen Zhujun Hou
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Prior studies of reproductive isolation among three varieties of Phacelia dubia have found that gene loci encoding isozymes of glutamate oxaloacetate transaminase (GOT) were associated with hybrid sterility. In the first part of this study, I generated three-way hybrids to determine if the genetic or chromosomal region linked to GOT encoding loci has major effects on hybrid sterility and if any observed effects were similar in different genetic backgrounds. In three-way crosses, F1 hybrids of two varieties were crossed to a third variety. Fertilities of three-way hybrids were estimated and their GOT genotypes were ascertained by starch gel electrophoresis. A …