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Construction Of A New Model System In Saccharomyces Cerevisa To Study Yopo And Identify Its Targets, Laura Nejedlik
Construction Of A New Model System In Saccharomyces Cerevisa To Study Yopo And Identify Its Targets, Laura Nejedlik
Dissertations
Yersinia species use a type three secretion system (TTSS) to inject Yersinia outer proteins (Yops) into the target cell. The effector YopO is a serine/threonine kinase which upon association with actin becomes active. However, activation of the kinase is not required for actin disruption. YopO also contains a Rho/Rac binding domain, but it is unclear how it affects these proteins.
We have established an expression system in Saccharomyces cerevisiae to study YopO. The expression vector contains a GAL1 promoter for inducible expression of YopO as well as a V5 epitope used for visualization of each Yop. We have shown that …
Characterization Of The Ap-1 And Nf-Kappa B Transcription Factors In The U-87 Mg Astrocytoma Cell Line, Denise L. Smith
Characterization Of The Ap-1 And Nf-Kappa B Transcription Factors In The U-87 Mg Astrocytoma Cell Line, Denise L. Smith
Dissertations
Growth factors, signaling molecules and transcription factors are frequently mutated in cancer and platelet-derived growth factor (PDGF) has been shown to be over-expressed in most gliomas. In the astrocytoma cell line U-87 MG, the over-expression of PDGF has been shown to lead to over-expression of transcription factors activator-protein-1 (AP-1) and nuclear factor-kappa B (NF-κB), and it is believed that the individual subunits of AP-1 (c-Jun, JunB, JunD, c-Fos, FosB, Fra-1, and Fra-2) and NF-κB (c-Rel, p50, p52, p65, and RelB) play different roles in proliferation, survival and differentiation in the U-87 MG cell line.
To assess these different roles, transient …