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Design And Construction Of Single-Spacer Type-Iv Crispr Sequences, Michael Adams, Hannah Domgaard, Ryan Jackson
Design And Construction Of Single-Spacer Type-Iv Crispr Sequences, Michael Adams, Hannah Domgaard, Ryan Jackson
Biology Posters
CRISPR systems are RNA-guided microbial adaptive immune systems that have been repurposed for applications in medicine, pharmacology, and agriculture. The hallmark of CRISPR immune systems is the CRISPR sequence contained in the host chromosome, which consists of short direct-repeats of 20-40 bases, followed by unique spacer sequences of about the same length. In 2005 several bioinformatics groups discovered that CRISPR spacer sequences in bacteria and archaea were identical to known microbial pathogens such as viruses and plasmids, suggesting that CRISPRs serve as a molecular memory of encounters with foreign nucleic acid. Since 2005 basic research on these systems has revealed …
Design Of A BiobrickTm Compatible Gene Expression System For Rhodobacter Sphaeroides, Junling Huo
Design Of A BiobrickTm Compatible Gene Expression System For Rhodobacter Sphaeroides, Junling Huo
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The concept of introducing engineering principles of abstraction and standardization into synthetic biology has received increasing attention in the past several years and continues to be in the forefront of synthetic biology. One direction being pursued by synthetic biologists is creation of modular biological parts (BioBrickTM) that can be readily synthesized and mixed together in different combinations. However, most standard BioBrickTM parts in the Registry were designed for E. coli, although synthesis of specific BioBrickTM parts for other bacteria, such as for yeast and cyanobacteria, have begun. Besides, at the present time, there are only …