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Morphological Effects Of Glycosaminoglycans On Calcium Oxalate Monohydrate Crystals, Y. Shirane, Y. Kurokawa, Y. Sumiyoshi, S. Kagawa
Morphological Effects Of Glycosaminoglycans On Calcium Oxalate Monohydrate Crystals, Y. Shirane, Y. Kurokawa, Y. Sumiyoshi, S. Kagawa
Scanning Microscopy
The effects of individual glycosaminoglycan (GAG) species on calcium oxalate monohydrate (COM) crystal growth were studied in vitro by the observation of crystal morphology grown in a supersaturated calcium oxalate solution in the presence of GAGs using optical and scanning electron microscopes. GAGs affected the morphology of COM crystals differently depending on the species. The growth rates of the crystals formed in the presence of chondroitin-6-sulfate (ChS-C) were higher in length and lower in width and thickness than those of control crystals. The incorporation of dermatan sulfate or heparin into the crystals formed in the presence of these GAGs was …
Low Concentrations Of Mercury Induce Changes In Ion Composition Of Cultured Myoblasts, Joanna Wroblewski, Jan Ekstrand, Romuald Wroblewski
Low Concentrations Of Mercury Induce Changes In Ion Composition Of Cultured Myoblasts, Joanna Wroblewski, Jan Ekstrand, Romuald Wroblewski
Scanning Microscopy
The effects of low concentrations (1 pM, 1 nM, 1 μM) of mercuric chloride on ion distribution in cultured myoblasts were analysed by energy dispersive X-ray microanalysis. An increase in intracellular sodium concentration was observed five minutes after addition of HgC12 to the culture medium. This increase was dose dependent and accompanied by a transient decrease in potassium concentration. Exposure to 1 nM and 1 μM HgC12 led to a two-fold increase in the cytoplasmic chlorine concentration. The higher HgC12 concentration (1 μM) induced morphological alterations in the form of cell membrane blebs, perforations and shrinkage or flattening of the …
Vitamin E Deficiency As A Model Of Precocious Brain Aging: Assessment By X-Ray Microanalysis And Morphometry, Carlo Bertoni-Freddari, Patrizia Fattoretti, Ugo Caselli, Roberta Paoloni, William Meier-Ruge
Vitamin E Deficiency As A Model Of Precocious Brain Aging: Assessment By X-Ray Microanalysis And Morphometry, Carlo Bertoni-Freddari, Patrizia Fattoretti, Ugo Caselli, Roberta Paoloni, William Meier-Ruge
Scanning Microscopy
Vitamin E (α-tocopherol) is a known biological antioxidant able to quench the lipid peroxidation chain and to protect the cellular structures (e.g., plasma membranes) from the attack of free radicals which are reported to play a primary role in aging. To assess whether the absence of α-tocopherol from the diet of young laboratory animals may be considered a reliable model of precocious brain aging, intracellular ionic content of brain cortex pyramidal cells, ultrastructural features of synaptic contact zones, synaptic mitochondria and perykarial mitochondria positive to the succlillc dehydrogenase (SDH) histochemical reaction with copper ferrocyanide have been investigated by X-ray microanalysis …
Early Elemental And Ionic Changes In Cultured Cells After Stimulation With Epidermal Growth Factor, Julian E. Beesley, Simon R. Hall, Martin J. Page, Karen Affleck
Early Elemental And Ionic Changes In Cultured Cells After Stimulation With Epidermal Growth Factor, Julian E. Beesley, Simon R. Hall, Martin J. Page, Karen Affleck
Scanning Microscopy
Stimulation of A43 l cells (a human vulval epidermal cell line) with 50 ng/ml of epidermal growth factor (EGF) in the presence of 1. 7 mM extracellular calcium produced a sharp and sustained rise in intracellular ionic Ca2+, increased elemental Na, decreased K and a rise in Ca. In the absence of extracellular calcium, the initial Ca2+ rise remained but the sustained elevation of intracellular Ca2+ was abolished, Na and K fluxes were variable and the Ca did not change. Increased Na and decreased K was marked at 2 minutes and returned to the control value after 60 minutes. The …