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Biology

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Utah State University

1995

Ultrastructure

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The Interaction Of Laser Energy With Ureter Tissues In A Long Term Investigation, U. Stratmann, K. Schaarschmidt, R. R. Lehmann, A. Heinze, G. H. Willital, E. Unsold Jun 1995

The Interaction Of Laser Energy With Ureter Tissues In A Long Term Investigation, U. Stratmann, K. Schaarschmidt, R. R. Lehmann, A. Heinze, G. H. Willital, E. Unsold

Scanning Microscopy

This study investigates tissue responses after laser irradiation of the rabbit ureter, which serves as an experimental model for rectourogenital fistulae of children. Twenty-five rabbit ureters were irradiated intraluminally by a Nd:YAG laser 1320 nm (2 Watt, 20 seconds and 3 Watt, 8 seconds) via an applicator with radialsymmetrical light distribution. Immediately, 2 weeks, 4 weeks, 8 weeks, and 16 weeks after irradiation, the ureters were X-rayed with contrast solution and prepared for light and transmission electron microscopy. For the parameters employed, no apparent morphological differences could be observed. Immediately, the central laser zone showed a transmural therrnonecrosis prevailed by …


Cell Surface Changes Of Hemopoietic Cells During Normal And Leukemic Differentiation: An Immuno-Scanning Electron Microscopy Study, D. Soligo, E. P. De Harven, N. Quirici, L. Caneva, G. Lambertenghi Deliliers Mar 1995

Cell Surface Changes Of Hemopoietic Cells During Normal And Leukemic Differentiation: An Immuno-Scanning Electron Microscopy Study, D. Soligo, E. P. De Harven, N. Quirici, L. Caneva, G. Lambertenghi Deliliers

Scanning Microscopy

Hemopoietic cells display a wide range of cell surface antigens which are either lineage specific or acquired during differentiation. Monoclonal antibodies can be used, in conjunction with colloidal gold markers, to identify under the scanning electron microscopy (SEM) at the single cell level, specific lineage or maturation stages in the hemopoietic bone marrow. Normal bone marrow cells, either gradient separated or purified by immuno-magnetic methods and leukemic cell samples, which can be considered as "frozen" stages of hemopoietic differentiation, have been studied with this method. Typical cell surface morphologies, which characterize immature progenitor cells and cells committed or differentiated towards …


Notes On The Microstructure Of The Nautilus Shell, P. R. Mitchell, P. P. Phakey Feb 1995

Notes On The Microstructure Of The Nautilus Shell, P. R. Mitchell, P. P. Phakey

Scanning Microscopy

The shell of the Nautilus was examined using scanning electron microscopy, transmission electron microscopy and polarized light microscopy. The structure consisted of two major layers: a porcellaneous outer layer and a nacreous inner layer. Most of the porcellaneous layer was composed of granular crystals randomly distributed with a substructure suggestive of bundles of acicular crystallites. A separate prismatic sublayer of the porcellaneous material was composed of a more regular arrangement of crystals. The nacre was composed of alternating crystalline lamellae and films of organic material. The lamellae were formed of many polygonal crystal platelets. The growth surface of the nacre …


Scanning Electron Microscopy And Transmission Electron Microscopy Aspects Of Synergistic Antitumor Activity Of Vitamin C - Vitamin K3 Combinations Against Human Prostatic Carcinoma Cells, Jacques Gilloteaux, James M. Jamison, Meenaxi Venugopal, David Giammar, Jack L. Summers Jan 1995

Scanning Electron Microscopy And Transmission Electron Microscopy Aspects Of Synergistic Antitumor Activity Of Vitamin C - Vitamin K3 Combinations Against Human Prostatic Carcinoma Cells, Jacques Gilloteaux, James M. Jamison, Meenaxi Venugopal, David Giammar, Jack L. Summers

Scanning Microscopy

A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K3 (Vit K3), or vitamin C:vitamin K3 combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K3 alone exhibited antitumor activity, but only at elevated doses. When Vit C and Vit K3 were combined at a C:K3 ratio of 100:1 and administered to the carcinoma cells, the 50% cytotoxic concentrations (CD50) of the vitamins decreased 10-to 60-fold. Subsequently, the DU145 cells were examined with transmission and scanning electron microscopy (TEM and SEM) following …