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Biology

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Utah State University

1986

Pollen

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A Summary Of Ion Beam Etching Of Biological Material With Special Reference To The Saddle Field Source, D. Claugher Apr 1986

A Summary Of Ion Beam Etching Of Biological Material With Special Reference To The Saddle Field Source, D. Claugher

Scanning Electron Microscopy

A brief review of etching of biological materials using radio frequency, ion beam and electrostatic focused sources. Results of experiments using fast atom sources to dissect pollen, diatoms, protozoa and small arthropods, indicate that they produce a much smoother result which enables investigations to be carried out at high magnifications with the SEM.


Plant Ultrastructure In The Scanning Electron Microscope, Susan H. Barnes, Stephen Blackmore Mar 1986

Plant Ultrastructure In The Scanning Electron Microscope, Susan H. Barnes, Stephen Blackmore

Scanning Electron Microscopy

Preparative techniques which have been used to study internal details of plant cells in the scanning electron microscope are reviewed. A number of methods have previously been described which involve selective extraction of materials from freeze-fractured surfaces and can be referred to as freeze-fracture and cytoplasmic maceration. One of these techniques which involves an extended period of cytoplasmic maceration with dilute osmium tetroxide has been applied to the study of Cichorium intybus (chicory) pollen ontogeny. The results obtained, including changes in the numbers of mitochondria and the form of endoplasmic reticulum during the course of development demonstrate the value of …