Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
Structural Features Of Isolated, Fractionated Bone Marrow Endothelium Compared To Sinus Endothelium In Situ, Seiji Irie, Mehdi Tavassoli
Structural Features Of Isolated, Fractionated Bone Marrow Endothelium Compared To Sinus Endothelium In Situ, Seiji Irie, Mehdi Tavassoli
Scanning Electron Microscopy
Structural features of isolated, fractionated rat bone marrow endothelium were compared to those of marrow sinus endothelium in situ. Marrow endothelium was purified, first by density gradient sedimentation on Percoll and then subjected to centrifugal elutriation. Using antifactor VIII antibody staining (indirect immunofluorescent method), preparations of greater than 50% purified endothelium were obtained. By SEM, these cells were about 10 μm in size and showed smooth surface and numerous invaginations. These features were also observed in the in situ endothelium obtained by perfusion-fixation and freeze-cracking. In addition, in situ endothelium displayed numerous hemopoietic cells in migration through the endothelium. By …
Ultrastructural Studies Of Intercellular Contacts (Junctions) In Bone Marrow. A Review, Ferrell R. Campbell
Ultrastructural Studies Of Intercellular Contacts (Junctions) In Bone Marrow. A Review, Ferrell R. Campbell
Scanning Electron Microscopy
This paper reviews ultrastructural studies of the intercellular contacts or junctions between cells of the bone marrow. Studies using tannic acid and glutaraldehyde as a fixative have shown pentalaminar complexes between many types of cells in marrow of mice and chicks. These intercellular contacts occur between adjacent stromal cells, between stromal cells and developing blood cells and, in marrow of mice, between migrating blood cells and cells of the sinusoidal wall. Because of their location and widespread occurrence, it is believed these contacts may represent a type of adherent junction helping to maintain an orderly arrangement of blood cells and …