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Temporal And Biochemical Aspects Of Sindbis Virus Dissemination In The Mosquito Host, Kristen A. Ciano Jan 2010

Temporal And Biochemical Aspects Of Sindbis Virus Dissemination In The Mosquito Host, Kristen A. Ciano

UNF Graduate Theses and Dissertations

The prototype Alphavirus, Sindbis virus (SIN), relies on cyclic transmission between the mosquito and vertebrate hosts in order to be maintained in nature. This broad host range suggests that alphaviruses use a universally expressed molecule for attachment. Heparan sulfate proteoglycan (HSPG), a ubiquitous SIN receptor present on the cell surface of most eukaryotic cells, has been reported in the salivary glands and midguts of mosquitoes. These organs are essential for virus transmission from this hematophageous invertebrate. Variable host cell response in the mosquito following intrathoracic inoculation with SIN has been documented. In this study, per os infection of Aedine …


Transepithelial D-Glucose And D-Fructose Transport Across Lobster Intestine, Ijeoma Ebelechukwu Obi Jan 2010

Transepithelial D-Glucose And D-Fructose Transport Across Lobster Intestine, Ijeoma Ebelechukwu Obi

UNF Graduate Theses and Dissertations

The mechanisms of transepithelial absorption of dietary D-glucose and D-fructose in the American lobster, Homarus americanus, were investigated in this study in order to determine whether sugar transport proteins have been conserved throughout evolution. Whole lobster intestine was isolated and mounted in a perfusion chamber to determine transepithelial mucosal to serosal (MS) and serosal to mucosal (SM) mechanisms of 3H-D-glucose and 3H-D-fructose transport across the intestine. Unidirectional MS and SM fluxes were measured by adding variable concentrations of 3H-D-glucose and 3H-D-fructose (2.5 to 50uM) to either the perfusate or the bath respectively and sampling the …


Arthropod Biodiversity In Response To The Restoration Of Former Pine Plantations, John Curtis Burkhalter Jan 2010

Arthropod Biodiversity In Response To The Restoration Of Former Pine Plantations, John Curtis Burkhalter

UNF Graduate Theses and Dissertations

The goal of ecological restoration is to return degraded or altered ecosystems to their pre-disturbed state with respect to ecosystem structure, function, and composition. In the current study the research objective was to reestablish high levels of biodiversity on two restored pine plantations as well as restore a native pine flatwoods ecosystem. Managed, even-aged pine flatwoods are now the most extensive ecosystem in North Florida, comprising approximately 70% of the forested landscape. Slash pine trees were thinned in the experimental plots to restore the natural slash pine density of native ecosystems. In addition to the thinning treatments, experimental plots have …


Seeking Generalities In Salt Stress Effects On Herbivores: A Multi-Species Approach, Christy Marie Foust Jan 2010

Seeking Generalities In Salt Stress Effects On Herbivores: A Multi-Species Approach, Christy Marie Foust

UNF Graduate Theses and Dissertations

Stress has been identified as one of the primary factors influencing the structure of food webs, but few conclusions about the effects of stress on community dynamics have emerged. This study examined generalities that exist between salt stress and insect herbivore responses. Ambient salinity was artificially increased by adding 1.3 kg/m2 of salt to six different plant species: Avicennia germinans, Baccharis halimifolia, Batis maritima, Borrichia frutescens, Iva frutescens, and Spartina alterniflora. By performing a multiple species examination, stress responses were examined to determine if consistencies within and between plant type, ambient salinity conditions, and host plant species (among other criteria) …