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Regulation Of Haplid Phenotypes In Ustilago Maydis By Ammonium Transporters And Components Of The B Mating Locus., Rena Margaret Wallen Aug 2017

Regulation Of Haplid Phenotypes In Ustilago Maydis By Ammonium Transporters And Components Of The B Mating Locus., Rena Margaret Wallen

Electronic Theses and Dissertations

Fungi that can switch from budding to a filamentous infectious state have evolved mating type loci. Ustilago maydis, the maize pathogen, must mate with compatible partners possessing different alleles at two mating type loci for successful host infection. The a locus encodes pheromones and receptors, while the b locus encodes subunits of a heterodimeric transcription factor that regulates expression of virulence genes. Mating is triggered by environmental signals, including nutrient deprivation. My goal was to determine the fate of nitrogen starved haploid cells without a compatible mating partner. On solid low ammonium media, wild-type U. maydis filaments. I examined …


Social Dynamics, Network Structure, And Information Diffusion In Fish Shoals., Matthew Jerome Hasenjager May 2017

Social Dynamics, Network Structure, And Information Diffusion In Fish Shoals., Matthew Jerome Hasenjager

Electronic Theses and Dissertations

Animal populations are often highly structured, with individuals differing in terms of whom they interact with and how frequently they do so. The resulting pattern of relationships constitutes a population’s social network. In this dissertation, I examine how environmental variation can shape social networks and influence information flow within them. In Chapter I, I review the history of social network analysis in animal behavior research, and discuss recent insights generated by network approaches in behavioral ecology. I focus on the fields of: social learning, collective behavior, animal personalities, and cooperation. Animal network studies are often criticized for a lack of …


Function Of The Enterobactin Operon Of A. Actinomycetemcomitans In The Presence Of Catecholmines And Iron., Taylor Johnson May 2017

Function Of The Enterobactin Operon Of A. Actinomycetemcomitans In The Presence Of Catecholmines And Iron., Taylor Johnson

Electronic Theses and Dissertations

Background: Aggregatibacter actinomycetemcomitans (Aa) possesses a two-component system, QseBC, which is key in biofilm formation/virulence and is activated in the presence of iron-catecholamine complexes. Aa does not synthesize enterobactin, a catechol-based siderophore, yet possesses the machinery for recognition and uptake. We hypothesize that Aa is able to acquire iron by catecholamine mediation through the enterobactin receptor/transporter. Methods: By insertional mutation, we attempted to delete the enterobactin permease from the genome using suicide vector pJT1. A growth curve of truncated mutant ΔfepA was also conducted to observe growth in the presence of iron and catecholamines. Statistical significance was determined by ANOVA …