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Articles 1 - 11 of 11
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A High-Content Multiplexed Screening Platform For The Evaluation And Manipulation Of Force And Fatigue Of Adult Derived Skeletal Muscle Myotubes In Defined Serum-Free Medium, Christopher Mcaleer
A High-Content Multiplexed Screening Platform For The Evaluation And Manipulation Of Force And Fatigue Of Adult Derived Skeletal Muscle Myotubes In Defined Serum-Free Medium, Christopher Mcaleer
Electronic Theses and Dissertations
The overall focus of this project has two parts: First, was to develop a protocol utilizing serum-free media formulations and defined plating and culture techniques to create functional in vitro myotubes derived from adult skeletal muscle satellite cells. The second was to manipulate the inherent muscle parameters such as force output and fatigue of these myotubes by employing exercise regimes or by small molecule application. The importance of serum-free medium use for in vitro cultures is becoming increasingly important in creating functional systems that can be validated for drug testing by the Food and Drug Administration (FDA). Also, the study …
Identifying Inundation-Driven Effects Among Intertidal Crassostrea Virginica In A Commercially Important Gulf Of Mexico Estuary, Joshua Solomon
Identifying Inundation-Driven Effects Among Intertidal Crassostrea Virginica In A Commercially Important Gulf Of Mexico Estuary, Joshua Solomon
Electronic Theses and Dissertations
Sea level rise and changing storm frequency and intensity resulting from climate change create tremendous amounts of uncertainty for coastal species. Intertidal species may be especially affected since they are dependent on daily inundation and exposure. The eastern oyster Crassostrea virginica is an economically and biologically important sessile intertidal species ranging from Canada to the Gulf of Mexico. Declines and changes in distribution of oyster populations has forced commercial harvesting to spread from subtidal to intertidal reefs. We investigated the potential responses of intertidal C. virginica to sea level rise, and the response of larval settlement to sedimentation which is …
Testing For Isolation In Florida Raccoons (Procyon Lotor) Using Phylogenetics And Population Genetic Structure, Alexa Trujillo
Testing For Isolation In Florida Raccoons (Procyon Lotor) Using Phylogenetics And Population Genetic Structure, Alexa Trujillo
Electronic Theses and Dissertations
Molecular data are useful in determining if populations are isolated and for species delimitation. Researchers and managers currently recognize five subspecies of raccoons (Procyon lotor) in Florida, based largely on perceived geographic isolation due to the island ranges of four subspecies. In this study, I provide the first estimate of phylogenetic relationships and population divergences within Florida raccoons using a molecular dataset. I analyze the mitochondrial control region, cytochrome b gene, and eight nuclear microsatellite loci to test two hypotheses: 1) the five, morphologically and geographically-defined subspecies of raccoon in Florida represent genetically distinct populations and (2) due to differing …
Development Of Human And Rodent Based In Vitro Systems Toward Better Translation Of Bench To Bedside In Vivo Results, Bonnie Berry
Development Of Human And Rodent Based In Vitro Systems Toward Better Translation Of Bench To Bedside In Vivo Results, Bonnie Berry
Electronic Theses and Dissertations
Prospective medicinal compounds progress through multiple testing phases before becoming licensed drugs. Testing of novel compounds includes a preclinical phase where the potential therapeutic is tested in vitro and/or in animal models in vivo to predict its potential efficacy and/or toxicity in humans. The failure of preclinical models to accurately predict human drug responses can lead to potentially dangerous compounds being administered to humans, or potentially beneficial compounds being kept in development abeyance. Moreover, inappropriate choice in model organism for studying disease states may result in pushing forward inappropriate drug targets and/or compounds and wasting valuable time and resources in …
Genetic Structure And Demographic Analysis Of Key Deer (Odocoileus Virginianus Clavium), Vicki Villanova
Genetic Structure And Demographic Analysis Of Key Deer (Odocoileus Virginianus Clavium), Vicki Villanova
Electronic Theses and Dissertations
Recent improvements in genetic analyses have paved the way in using molecular data to answer questions regarding evolutionary history, genetic structure, and demography. Key deer are a federally endangered subspecies assumed to be genetically unique (based on one allozyme study), homogeneous, and have a female-biased population of approximately 900 deer. I used 985bp of the mitochondrial cytochrome b gene and 12 microsatellite loci to test two hypotheses: 1) if the Moser Channel is a barrier to gene flow, I should expect that Key deer are differentiated and have reduced diversity compared to mainland deer and (2) if isolation on islands …
Does The Journey Matter More Than The Destination? The Contribution Of Geospatial And Local Conditions To Invasive Pomacea Maculata Distribution Across Ranchland Wetlands, Steffan Pierre
Electronic Theses and Dissertations
Geospatial characteristics such as isolation and avenues of connectivity influence an invader's pattern of dispersal and distribution. However many examinations of invasion success ignore the contribution of dispersal to patterns of invasion and focus only on the local environmental/habitat factors. This study examines the interaction of geospatial characteristics, that may influence dispersal, and local environmental factors, that may govern successful occupation, on the likelihood of invasion (invasability) of wetlands within an agriculturally modified landscape. I examined the current invasion of seasonal wetlands in south-central Florida ranchland by non-native apple snails (Pomacea maculata (Ampullariidae)) as a model system for understanding this …
A Binary Approach For Selective Recognition Of Nucleic Acids And Proteins, Evan Cornett
A Binary Approach For Selective Recognition Of Nucleic Acids And Proteins, Evan Cornett
Electronic Theses and Dissertations
The design of probes for the selective recognition of biopolymers (nucleic acids and proteins) is a fundamental task for studying, diagnosing, and treating diseases. Traditional methods utilize a single component (small molecule or oligonucleotide) that binds directly to the target biopolymer. However, many biopolymers are unable to be targeted with this approach. The overarching goal of this dissertation is to explore a new, binary approach for designing probes. The binary approach requires two components that cooperatively bind to the target, triggering a recognition event. The requisite binding of two-components allows the probes to have excellent selectivity and modularity. The binary …
Quantifying The Effects Of Boat Wakes On Intertidal Oyster Reefs In A Shallow Estuary, Donna Campbell
Quantifying The Effects Of Boat Wakes On Intertidal Oyster Reefs In A Shallow Estuary, Donna Campbell
Electronic Theses and Dissertations
There have long been concerns about the negative impacts of recreational boating activity in the Indian River Lagoon system (IRL), especially in Mosquito Lagoon (ML), the northernmost part of the IRL. My research is focused on the impacts of boat wakes on intertidal reefs formed by the eastern oyster, Crassostrea virginica. There has been a 24% loss of oyster habitat in ML since 1943, where natural oyster reefs have been replaced by dead oyster reefs which do not serve the same ecological function. While there is anecdotal and correlative evidence that this loss is a result of boat wakes, no …
A Socio-Economic Assessment Of Marine Turtle Eco-Tourism, Kendra Cope
A Socio-Economic Assessment Of Marine Turtle Eco-Tourism, Kendra Cope
Electronic Theses and Dissertations
Marine turtles have historically contributed to economic activity through consumptive harvest for food, tools, and decorative objects. Only recently have they begun to benefit humans economically through non-consumptive roles, primarily as a focal point of educational eco-tourism. In recent years, the annual number of turtle walks conducted around the Archie Carr National Wildlife Refuge (ACNWR) has risen. This expansion contributed to a statistically significant increase in attendance from 2,162 in 2001 to 3,047 participants in 2014. I examined the regional economic impacts of marine turtle eco-tourism around the ACNWR using social surveys and an economic impact analysis tool. IMPLAN, an …
Modeling And Analysis Of A Three-Species Food Web With Facilitated And Intraguild Predation, Joshua Castro
Modeling And Analysis Of A Three-Species Food Web With Facilitated And Intraguild Predation, Joshua Castro
Electronic Theses and Dissertations
Biotic interactions are known to shape natural community assemblages and biodiversity. Positive interactions such as facilitation have recently received attention in ecological food webs. Mechanistic models have improved our understanding of these complex food web interactions. Here, focus is given to a three-species food web system with a beach dune natural community in mind. In the last decade, there has been a series of studies investigating intraguild predation between two major loggerhead sea turtle nest predators, North American raccoons and Atlantic ghost crabs. Studies have also highlighted that ghost crab predation assists raccoons in finding nests (i.e., facilitated predation). However, …
The Effect Of Landscape Variables On Adult Mosquito (Diptera:Culicidae)Diversity And Behavior, Caitlyn Debevec
The Effect Of Landscape Variables On Adult Mosquito (Diptera:Culicidae)Diversity And Behavior, Caitlyn Debevec
Electronic Theses and Dissertations
Diseases vectored by mosquitoes cause millions of deaths each year. In modern times Florida*s disease risk has been reduced due to efforts to lessen the prevalence of mosquitoes through habitat modification of non-adults. With emerging diseases (i.e. Dengue and Chikunguya) encroaching into Florida from the Caribbean, this traditional approach may not be enough. Alternatively, we can better understand the ecology of how disease works in an ecosystem. One possible way is through the Dilution Effect, which states that the more species that are in a system the lower the chance for zoonosis. This project models mosquito diversity across regions, land …