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Evidence For Phytoremediation And Phytoexcretion Of Nto From Industrial Wastewater By Vetiver Grass, Abhishek Roychowdhury, Pallabi Mukherjee, Saumik Panja, Rupali Datta, Christos Christodoulatos, Dibyendu Sarkar Dec 2020

Evidence For Phytoremediation And Phytoexcretion Of Nto From Industrial Wastewater By Vetiver Grass, Abhishek Roychowdhury, Pallabi Mukherjee, Saumik Panja, Rupali Datta, Christos Christodoulatos, Dibyendu Sarkar

Michigan Tech Publications

The use of insensitive munitions such as 3-nitro-1,2,4-triazol-5-one (NTO) is rapidly increasing and is expected to replace conventional munitions in the near future. Various NTO treatment technologies are being developed for the treatment of wastewater from industrial munition facilities. This is the first study to explore the potential phytoremediation of industrial NTO-wastewater using vetiver grass (Chrysopogon zizanioides L.). Here, we present evidence that vetiver can effectively remove NTO from wastewater, and also translocated NTO from root to shoot. NTO was phytotoxic and resulted in a loss of plant biomass and chlorophyll. The metabolomic analysis showed significant differences between treated and …


Maize Microrna166 Inactivation Confers Plant Development And Abiotic Stress Resistance, Na Li, Tianxiao Yang, Zhanyong Guo, Qiusheng Wang, Mao Chai, Mingbo Wu, Xiaoqi Li, Weiya Li, Guangxian Li, Jihua Tang, Guiliang Tang Dec 2020

Maize Microrna166 Inactivation Confers Plant Development And Abiotic Stress Resistance, Na Li, Tianxiao Yang, Zhanyong Guo, Qiusheng Wang, Mao Chai, Mingbo Wu, Xiaoqi Li, Weiya Li, Guangxian Li, Jihua Tang, Guiliang Tang

Michigan Tech Publications

MicroRNAs are important regulators in plant developmental processes and stress responses. In this study, we generated a series of maize STTM166 transgenic plants. Knock-down of miR166 resulted in various morphological changes, including rolled leaves, enhanced abiotic stress resistance, inferior yield-related traits, vascular pattern and epidermis structures, tassel architecture, as well as abscisic acid (ABA) level elevation and indole acetic acid (IAA) level reduction in maize. To profile miR166 regulated genes, we performed RNA-seq and qRT-PCR analysis. A total of 178 differentially expressed genes (DEGs) were identified, including 118 up-regulated and 60 down-regulated genes. These DEGs were strongly enriched in cell …


The Modular Expression Patterns Of Three Pigmentation Genes Prefigure Unique Abdominal Morphologies Seen Among Three Drosophila Species, William A. Dion, Mujeeb O. Shittu, Tessa Steenwinkel, Komal K.B. Raja, Prajakta P. Kokate, Thomas Werner Dec 2020

The Modular Expression Patterns Of Three Pigmentation Genes Prefigure Unique Abdominal Morphologies Seen Among Three Drosophila Species, William A. Dion, Mujeeb O. Shittu, Tessa Steenwinkel, Komal K.B. Raja, Prajakta P. Kokate, Thomas Werner

Michigan Tech Publications

To understand how novel animal body colorations emerged, one needs to ask how the development of color patterns differs among closely related species. Here we examine three species of fruit flies – Drosophila guttifera (D. guttifera), D. palustris, and D. subpalustris – displaying a varying number of abdominal spot rows. Through in situ hybridization experiments, we examine the mRNA expression patterns for the pigmentation genes Dopa decarboxylase (Ddc), tan (t), and yellow (y) during pupal development. Our results show that Ddc, t, and y are co-expressed in modular, identical patterns, each foreshadowing the adult abdominal spots in D. guttifera, D. …


Evolution Of Breeding Plumages In Birds: A Multiple-Step Pathway To Seasonal Dichromatism In New World Warblers (Aves: Parulidae), Ryan S. Terrill, Glenn F. Seeholzer, Jared D. Wolfe Sep 2020

Evolution Of Breeding Plumages In Birds: A Multiple-Step Pathway To Seasonal Dichromatism In New World Warblers (Aves: Parulidae), Ryan S. Terrill, Glenn F. Seeholzer, Jared D. Wolfe

Michigan Tech Publications

Ecology and Evolution published by John Wiley & Sons Ltd Many species of birds show distinctive seasonal breeding and nonbreeding plumages. A number of hypotheses have been proposed for the evolution of this seasonal dichromatism, specifically related to the idea that birds may experience variable levels of sexual selection relative to natural selection throughout the year. However, these hypotheses have not addressed the selective forces that have shaped molt, the underlying mechanism of plumage change. Here, we examined relationships between life-history variation, the evolution of a seasonal molt, and seasonal plumage dichromatism in the New World warblers (Aves: Parulidae), a …


The Making Of Transgenic, Mujeeb Olushola Shittu, Tessa Steenwinkel, Shigeyuki Koshikawa, Thomas Werner Apr 2020

The Making Of Transgenic, Mujeeb Olushola Shittu, Tessa Steenwinkel, Shigeyuki Koshikawa, Thomas Werner

Michigan Tech Publications

The complex color patterns on the wings and body of Drosophila guttifera (D. guttifera) are emerging as model systems for studying evolutionary and developmental processes. Studies regarding these processes depend on overexpression and downregulation of developmental genes, which ultimately rely upon an effective transgenic system. Methods describing transgenesis in Drosophila melanogaster (D. melanogaster) have been reported in several studies, but they cannot be applied to D. guttifera due to the low egg production rate and the delicacy of the eggs. In this protocol, we describe extensively a comprehensive method used for generating transgenic D. guttifera. Using the protocol described here, …


Spatial Contrasts In Hepatic And Biliary Pahs In Tilefish (Lopholatilus Chamaeleonticeps) Throughout The Gulf Of Mexico, With Comparison To The Northwest Atlantic, Susan M. Snyder, Jill A. Olin, Erin L. Pulster, Steven A. Murawski Mar 2020

Spatial Contrasts In Hepatic And Biliary Pahs In Tilefish (Lopholatilus Chamaeleonticeps) Throughout The Gulf Of Mexico, With Comparison To The Northwest Atlantic, Susan M. Snyder, Jill A. Olin, Erin L. Pulster, Steven A. Murawski

Michigan Tech Publications

A multinational demersal longline survey was conducted on the Gulf of Mexico continental shelf over the years 2015 and 2016 to generate a Gulf-wide baseline of polycyclic aromatic hydrocarbon (PAH) concentrations in demersal fishes. Tilefish (Lopholatilus chamaeleonticeps) were sampled in all regions of the Gulf of Mexico for biometrics, bile, and liver. Tilefish liver was also obtained from surveys in the northwest Atlantic Ocean for comparison. Liver tissues (n ¼ 305) were analyzed for PAHs and select alkylated homologs using QuEChERS extractions and gas chromatography tandem mass spectrometry. Bile samples (n ¼ 225) were analyzed for biliary PAH metabolites using …


Growing Biofuel Feedstocks In Copper-Contaminated Soils Of A Former Superfund Site, Virinder Sidhu, Dibyendu Sarkar, Rupali Datta Feb 2020

Growing Biofuel Feedstocks In Copper-Contaminated Soils Of A Former Superfund Site, Virinder Sidhu, Dibyendu Sarkar, Rupali Datta

Michigan Tech Publications

Copper mining in the Upper Peninsula of Michigan in the mid-19th century generated millions of tons of mining waste, called stamp sand, which was deposited into various offshoots of Lake Superior. The toxic stamp sand converted the area into barren, fallow land. Without a vegetative cover, stamp sand has been eroding into the lakes, adversely affecting aquatic life. Our objective was to perform a greenhouse study, to grow cold-tolerant oilseed crops camelina (Camelina sativa) and field pennycress (Thlaspi arvense) on stamp sand, for the dual purpose of biofuel production and providing a vegetative cover, thereby decreasing erosion. Camelina and field …


Effects Of Invasive Watermilfoil On Primary Production In Littoral Ones Of North-Temperate Lakes, Ryan R. Van Goethem, Casey Huckins, Amy Marcarelli Feb 2020

Effects Of Invasive Watermilfoil On Primary Production In Littoral Ones Of North-Temperate Lakes, Ryan R. Van Goethem, Casey Huckins, Amy Marcarelli

Michigan Tech Publications

Species invasions are changing aquatic ecosystems worldwide. Submerged aquatic macrophytes control lake ecosystem processes through their direct and indirect interactions with other primary producers, but how these interactions may be altered by macrophyte species invasions in temperate lakes is poorly understood. We addressed whether invasive watermilfoil (IWM) altered standing crops and gross primary production (GPP) of other littoral primary producers (macrophytes, phytoplankton, attached algae, and periphyton) in littoral zones of six Michigan lakes through a paired-plot comparison study of sites with IWM (standardized abundance 7–56%) compared to those with little or no IWM (standardized abundance 0–2%). We found that primary …


Copper-Rich “Halo” Off Lake Superior's Keweenaw Peninsula And How Mass Mill Tailings Dispersed Onto Tribal Lands, W. Charles Kerfoot, Noel Urban, Jaebong Jeong, Carol Maclennan, Sophia Ford Jan 2020

Copper-Rich “Halo” Off Lake Superior's Keweenaw Peninsula And How Mass Mill Tailings Dispersed Onto Tribal Lands, W. Charles Kerfoot, Noel Urban, Jaebong Jeong, Carol Maclennan, Sophia Ford

Michigan Tech Publications

Over a century ago, shoreline copper mills sluiced more than 64 million metric tonnes of tailings into Lake Superior, creating a “halo” around the Keweenaw Peninsula with a buried copper peak. Here we examine how tailings from one of the smaller mills (Mass Mill, 1902–1919) spread as a dual pulse across southern Keweenaw Bay and onto tribal L'Anse Indian Reservation lands. The fine (“slime clay”) fraction dispersed early and widely, whereas the coarse fraction (stamp sands) moved more slowly southward as a black sand beach deposit, leaving scattered residual patches. Beach stamp sands followed the path of sand eroding from …