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A Novel Rcc1-Like Protein Is A Crucial Regulator Of The Intraerythrocytic Cycle Of The Human Malaria Parasite, Plasmodium Falciparum., Marcus Davon Skaflen Dec 2013

A Novel Rcc1-Like Protein Is A Crucial Regulator Of The Intraerythrocytic Cycle Of The Human Malaria Parasite, Plasmodium Falciparum., Marcus Davon Skaflen

Masters Theses, 2010-2019

Malaria is a deadly infection caused by a single celled protozoan of the Plasmodium genus. Plasmodium spp. are transmitted to humans by mosquitoes, and initially invade the liver, but the disease is caused by the blood stage of the infection. Approximately 500 million cases of malaria are documented annually and over 1 million of those result in death. Plasmodium falciparum is the most lethal of five species known to infect humans. To further compound this problem, drug-resistant parasite strains have been documented for every currently available antimalarial drug, making the need to identify new drug targets more urgent than ever. …


Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon May 2013

Microbial Dynamics And Core Microbiome Of Red-Backed Salamanders (Plethodon Cinereus), Andrew Howard Loudon

Masters Theses, 2010-2019

Beneficial cutaneous bacteria on amphibians can protect against the lethal fungal disease chytridiomycosis, which has decimated many amphibian species. The stability of these bacterial communities likely influences health outcomes, and is investigated here for the first time. We describe the diversity of bacteria on red-backed salamanders (Plethodon cinereus) in the wild, and the stability of these communities over time in captivity using culture-independent Illumina sequencing. In the field, there was no correlation between the diversity of salamanders’ microbial communities and the diversity of their substrates’ microbial communities. Salamanders were brought into the laboratory to test for the effect of an …


The Potential For Replication And Transmission Of Antibiotic Resistance Plasmids In An E. Coli Population In Agriculturally Impacted Stream Sediment, Erika Gehr May 2013

The Potential For Replication And Transmission Of Antibiotic Resistance Plasmids In An E. Coli Population In Agriculturally Impacted Stream Sediment, Erika Gehr

Masters Theses, 2010-2019

The use of antibiotics in agriculture is thought to be a major cause of resistance in microorganisms found in the environment. Horizontal transfer of genetic information from transient to native and from native to transient bacterial populations may enhance the spread and recombination of resistance genes and might play a role in the formation of multi-resistant organisms in environmental reservoirs. Tetracycline resistance plasmids were compared using three isolation techniques -- traditional “endogenous” extraction from isolates, “exogenous” plasmid capture, and direct plasmid extraction from sediment --to determine the potential for plasmid born resistance in an E. coli population found in agriculturally-impacted …


Occurrence, Survival, And Persistence Of The Fecal Indicator Bacterium, Enterococcus Mundtii, In Soils Of A Contaminated Watershed, Marc Carpenter May 2013

Occurrence, Survival, And Persistence Of The Fecal Indicator Bacterium, Enterococcus Mundtii, In Soils Of A Contaminated Watershed, Marc Carpenter

Masters Theses, 2010-2019

The Clean Water Act of 1972 raised awareness of the extent of the pollution in U.S. waters and the need for monitoring and identifying sources of contamination. Surface water quality is routinely evaluated using fecal indicators, such as Enterococcus spp. Recent studies in a south Texas watershed showed that fecal bacteria were being transported to water via agricultural land runoff following rainfall, suggesting that soil may be a source of these bacteria. In this study, soils from fields under different crop covers were sampled to determine seasonal levels of enterococci and species of enterococci were identified using the BIOLOG™ Microbial …


Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz May 2013

Probiotic Bioaugmentation Of An Anti-Bd Bacteria, Janthinobacterium Lividum, On The Amphibian, Notophthalmus Viridescens: Transmission Efficacy And Persistence Of The Probiotic On The Host And Non-Target Effects Of Probiotic Addition On Ecosystem Components, Molly Bletz

Masters Theses, 2010-2019

Amphibians worldwide are threatened by the fungal disease chytridiomycosis, caused by the skin pathogen Batrachochytrium dendrobatidis. Mutualistic skin bacteria are a critical element in amphibians’ defenses against chytridiomycosis. Probiotic bioaugmentation of beneficial, anti-Bd bacteria on amphibians is a potential conservation strategies. Outdoor experimental ponds were used to investigate transmission efficacy and persistence of the anti-Bd bacteria, Janthinobacterium lividum, on the amphibian, Notophthalmus viridescens. More specifically, this research investigated whether a short-term individual bath, environmental bioaugmentation, or both are necessary to afford transmission and persistence of J. lividum on N. viridescens. Additionally, this research investigated the effectiveness of these different probiotic …