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Development Of A Molecular Assay For Caribbean Coral Identification, Elijah D. O'Cain

Electronic Theses and Dissertations

As coral cover has declined throughout the Caribbean, interest in the role that recruitment processes play in reef recovery has increased. Studies investigating these processes have been hampered by the inability to identify many species of coral larvae or recently settled recruits using morphology. In this study, the utility of targeting the non-coding internal transcribed spacer (ITS) regions with a multiplex PCR assay to identify common Caribbean coral species was explored. To design this assay, a database of ITS sequences was developed for 17 Caribbean scleractinian coral species that are important reef builders and/or common in the Florida Keys. It …