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Effect Of Stress On Mucin Expression In The Gastrointestinal Tract Of Mice., Bupe Martha Habiyambere
Effect Of Stress On Mucin Expression In The Gastrointestinal Tract Of Mice., Bupe Martha Habiyambere
Electronic Theses and Dissertations
This study investigated the effects of chronic stress on mucin expression in the GIT of mice. Sixteen ICR male mice were randomly divided into treated and control animals. Restraint stress was applied to the treated group for 21 days. Body weight (BW) changes and feed consumed (FC) were regularly recorded. After 21 days mice were euthanized and blood, GIT mucosa, and GIT sections were collected. Serum corticosterone levels were determined. The GIT sections were fixed, paraffin embedded, sectioned, and stained with AB-PAS to visualize goblet cells. Mucin expression was determined using qPCR. As expected stressed mice showed higher corticosterone than …
The Effects Of Betadine®, Polyvinylpyrrolidone (Pvp), And Iodine On Regeneration In The Planarian Dugesia Tigrina., Traci R. Shaffer
The Effects Of Betadine®, Polyvinylpyrrolidone (Pvp), And Iodine On Regeneration In The Planarian Dugesia Tigrina., Traci R. Shaffer
Electronic Theses and Dissertations
The freshwater planarian has a great capacity to regenerate and is an ideal animal model in the study of stem cell and regeneration biology. In this study planarian regenerating new tails were exposed to nonlethal doses of Betadine®, Polyvinylpyrrolidone (PVP), and Iodine. Betadine® is a topical antiseptic commonly used in the healthcare setting and may have a detrimental effect on wound healing. PVP is linked to iodine to create povidone-iodine, the active ingredient in Betadine®. Initially, a preliminary test was performed on the worms to determine the nonlethal concentrations of these chemicals . After this nonlethal concentration was determined, tails …