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Articles 1 - 12 of 12
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Evaluating A Test For Shedding Propensity Using Tape Lifts From Different Skin Locations, Xiao M. Chen
Evaluating A Test For Shedding Propensity Using Tape Lifts From Different Skin Locations, Xiao M. Chen
Student Theses
The shedding propensity of a person can assist data interpretation in casework when assessing the possibility of passive transfer for DNA analysis. Past studies on shedding propensity evaluated palmar skin (washed and unwashed) deposits. This study compared different skin locations with respect to shedding propensity, and explored the potential of tape-lifts as a skin surface collection method. Eight different skin types and samples were collected with adhesive tape disks from 28 participants over three non-consecutive days; the washed and unwashed fingers from both hands, toe, and arm, neck below ear, and nape. Samples were extracted, quantified, amplified, genotyped, and evaluated …
Three Lc-Ms Plant Metabolomics Studies Of Hop (Humulus) Species: Wild H. Neomexicanus, Drought Stress, And Agricultural Terroir, Taylan Morcol
Three Lc-Ms Plant Metabolomics Studies Of Hop (Humulus) Species: Wild H. Neomexicanus, Drought Stress, And Agricultural Terroir, Taylan Morcol
Dissertations, Theses, and Capstone Projects
The hop plant (Humulus L., Cannabaceae) is a dioecious, perennial, twining vine with a long history of human use. Nowadays, hop plants are generally grown for their inflorescences (“cones”), which are used in brewing for their phytochemical metabolites. Many of these metabolites are involved in plant stress response and communication. Genetics and environment are two major factors that affect plant metabolism. In three separate metabolomics studies, this project examined the effects of both genetic and environmental factors on hop phytochemistry.
In the first study, 23 hop genotypes were grown in two different locations in the Pacific Northwest region of …
Understanding The Influence Of Zinc On Grain Cadmium Accumulation And Bioaccessibility In Rice, Michael A. Tavarez
Understanding The Influence Of Zinc On Grain Cadmium Accumulation And Bioaccessibility In Rice, Michael A. Tavarez
Dissertations, Theses, and Capstone Projects
The effect of cadmium and zinc on mineral concentrations in three cultivars of rice
Interactions between the essential mineral zinc (Zn) and the toxic heavy metal cadmium (Cd) play an important role in regulating transport of both minerals to rice grains. Understanding these interactions is crucial for limiting cadmium and increasing zinc transfer to the food chain. Previous studies on the matter have had conflicting results suggesting synergistic and antagonistic relationships between the minerals. The goal of this work was to identify the effect of external cadmium and zinc on the uptake and translocation of both minerals from roots to …
The Structural And Functional Role Of Photosensing In Rgs-Lov Proteins, Zaynab Jaber
The Structural And Functional Role Of Photosensing In Rgs-Lov Proteins, Zaynab Jaber
Dissertations, Theses, and Capstone Projects
Light provides organisms with energy and spatiotemporal information. To survive and adapt, organisms have developed the ability to sense light to drive biochemical effects that underlie vision, entrainment of circadian rhythm, stress response, virulence, and many other important molecularly driven responses. Blue-light sensing Light-Oxygen-Voltage (LOV) domains are ubiquitous across multiple kingdoms of life and modulate various physiological events via diverse effector domains. Using a small molecule flavin chromophore, the LOV domain undergoes light-dependent structural changes leading to activation or repression of these catalytic and non-catalytic effectors. In silico analyses of high-throughput genomic sequencing data has led to the marked expansion …
Estrogen Modulation Of Vta Dopamine Neuron Physiology And Behavioral Responsivity To Variable Social Stressors, Mary R. Shanley
Estrogen Modulation Of Vta Dopamine Neuron Physiology And Behavioral Responsivity To Variable Social Stressors, Mary R. Shanley
Dissertations, Theses, and Capstone Projects
The behavioral output of different animals, or even the same animal in different contexts, is remarkably variable in response to the same external stimulus. This behavioral diversity is due to the complex integration of external and internal stimuli, through both neuronal and hormonal signals that selects the best behavioral response. By their nature as long-distance signaling molecules, hormones play a critical role in communicating information about internal states across the organism. Many hormones produced in the periphery target the central nervous system to modulate animal behavior, selecting for behaviors that are appropriate over behaviors that are maladaptive in that specific …
Molecular Mechanisms Underlying Cell Fate Choice Within Specific Retinal Lineages, Estie Schick
Molecular Mechanisms Underlying Cell Fate Choice Within Specific Retinal Lineages, Estie Schick
Dissertations, Theses, and Capstone Projects
During development, retinal progenitor cells (RPCs) divide to form all of the cell types that make up the retina. Multipotent RPCs are competent to generate all retinal cell types, while restricted RPCs form specific lineages of cells. In particular, one genetically-defined RPC type preferentially gives rise to cone photoreceptors and horizontal cells. Many of the mechanisms that are responsible for directing cell fate choice within this lineage are unknown. This thesis largely focuses on examining the development of specific cell types and subtypes from restricted RPCs and on investigating the gene regulatory events that underlie cone photoreceptor and horizontal cell …
Mitochondrial Distribution Of Glycine Receptors In Motor Neuron Cell Lines, Katsiaryna Milashevich
Mitochondrial Distribution Of Glycine Receptors In Motor Neuron Cell Lines, Katsiaryna Milashevich
Student Theses and Dissertations
Although non-essential, glycine plays an important role in major metabolic reactions and is most known for its anti-inflammatory effects. An accumulation of contemporary research has shown that glycine is able to stabilize membrane potential using glycine receptors at the cellular level and to protect mitochondrial function directly, whether it is from inflammation, heavy metal poisoning, or ischemia-induced neuroinflammation. In this research, the existence of a hypothetical mitochondrial glycine receptor is examined. Immunofluorescence imaging was used to examine the presence of the glycine receptor subunits alpha 1 and alpha 2 in both non- differentiated and differentiated neuroblastoma cell lines. The preliminary …
Cell Cycle Progression Effects Of Albumin, Sharmeen Uddin
Cell Cycle Progression Effects Of Albumin, Sharmeen Uddin
Dissertations, Theses, and Capstone Projects
Progression through G1 phase of the cell cycle is controlled largely by growth factors in early G1 and by nutrients in late G1 indicating sufficient raw material for cell division. We previously mapped a late G1 cell cycle checkpoint for lipids upstream from a mammalian target of rapamycin complex 1 (mTORC1)-mediated checkpoint and downstream from a mid-G1 checkpoint known as the Restriction point. We therefore investigated a role for lipids in progression through late G1 into S-phase. Quiescent BJ-hTERT human fibroblasts primed back into G1 with FBS treatment, were treated with a mixture of …
The C. Neoformans Cell Wall: A Scaffold For Virulence, Christine Chrissian
The C. Neoformans Cell Wall: A Scaffold For Virulence, Christine Chrissian
Dissertations, Theses, and Capstone Projects
Cryptococcus neoformans is a globally distributed opportunistic fungal pathogen and the causative agent of life threatening cryptococcal meningoencephalitis in immunocompromised individuals, resulting in ~180,000 deaths each year worldwide. A primary virulence-associated trait of this organism is the production of melanin. Melanins are a class of diverse pigments produced via the oxidation and polymerization of aromatic ring compounds that have a characteristically complex, heterogenous, and amorphous structure. They are synthesized by representatives of all biological kingdoms and share a multitude of remarkable properties such as the ability to absorb ultraviolet (UV) light and protect against ionizing radiation. Melanin production in fungi …
Chloroplast Genome Evolution In The Klebsormidiophyceae And Streptofilum, Sarah Glass
Chloroplast Genome Evolution In The Klebsormidiophyceae And Streptofilum, Sarah Glass
Theses
The Klebsormidiophyceae are a class of green microalgae found globally in both freshwater and terrestrial habitats. Morphology-based classification schemes of this class have been shown to be insufficient, particularly due to the simple morphology of these algae, the tendency of morphology to vary in culture versus in field conditions, and rampant morphological homoplasy. Molecular studies revealing cryptic diversity have renewed interest in this group. We sequenced the complete chloroplast genomes of taxa in the Klebsormidiophyceae representing the known taxonomic breadth of this class, as well as the chloroplast genomes of three strains of Streptofilum, a recently discovered algal lineage …
There’S More Than One Way To Beat The Heat: What Anal Probes Reveal About Thermal Responses In Neotropical Beetles (Coleoptera: Cerambycidae)., Emilie I. Dion
There’S More Than One Way To Beat The Heat: What Anal Probes Reveal About Thermal Responses In Neotropical Beetles (Coleoptera: Cerambycidae)., Emilie I. Dion
Dissertations and Theses
Increasing global temperatures have been linked to shifts in the distributions and preferred microhabitats of terrestrial species. This trend is predicted to impact tropical forest insects, which may already be exposed to conditions that bring them close to their thermal maxima. Tropical microhabitats are linked to vertical stratification, because the canopy is hotter, winder, and less humid than the ground. In ectothermic organisms, such as beetles, thermoregulation is dependent on morphological and behavioral traits. This study investigates woodboring cerambycid beetles, to determine how behavioral thermoregulation through microhabitat selection (vertical stratification) is correlated to morphological attributes (color lightness, size, and pubescence) …
Analyzing Ultrastructural Characteristics Of Post-Synaptic Targets Of Pre-Identified Presynaptic Boutons In Layer 3 And Layer 4 Of The Ferret Primary Visual Cortex, Donia Shehab
Dissertations and Theses
The purpose of this study was to analyze the size and ultrastructural characteristics of post-synaptic elements apposed to previously characterized pre-synaptic elements in layers 3 and 4 of the primary visual cortex (PVC). We are aiming to quantify a range of values for synaptic element morphologies as well as to correlate pre- and post-synaptic elements to find a set of rules that is prevalent in the visual cortex that can also be upheld in other mammals. We hypothesize that bigger pre-synaptic elements correlate to bigger synapses and bigger post-synaptic elements. We compared these two different layers because they receive synaptic …