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Spaceflight And Differential Gene Expression Analysis Of Mice Quadriceps Exposed To Microgravity, Tommy Nguyen
Spaceflight And Differential Gene Expression Analysis Of Mice Quadriceps Exposed To Microgravity, Tommy Nguyen
Master's Projects
he trip to Mars and back is planned for the next 20 years. Improvement in technology and research has allowed data analysis, at a larger scale, on spaceflight specimen. However, research involving spaceflight is decentralized, as research is spread across laboratories with different methodologies. NASA’S GeneLab is a public repository for spaceflight-related omics data and promotes centralizing spaceflight RNA-Seq studies using NASA’s RNA-Seq pipeline. Jonathan Oribello, a SJSU’s Bioinformatics graduate and now an employee at GeneLab, has implemented Nextflow to NASA’s RNA-Seq pipeline. The Nextflow RNA-Seq pipeline was ran on San Jose State University’s College of Science High Performance Computing …
Mrna-Sequencing Pipeline For Differential Gene Expression Analysis, Crystal Han
Mrna-Sequencing Pipeline For Differential Gene Expression Analysis, Crystal Han
Master's Projects
Endothelial cells (ECs) line the insides of blood vessels and play a key role in the coagulation and vascular repair system. Under normal circumstances, ECs are constantly expressing anticoagulants to prevent clots and maintain healthy blood flow. But when there is an injury to the vessel, endothelial cells become centrally involved in orchestrating the complex series of events that would lead to the clotting of the wound. Thus, ECs are dynamic and respond accordingly based on changes in their local environment. Unfortunately, endothelial cells can sometimes misinterpret cues from the environment and initiate coagulation when there is no vessel damage …
Differential Gene Expression Analysis Of Mice Thymi After Spaceflight, Roshani Codipilly
Differential Gene Expression Analysis Of Mice Thymi After Spaceflight, Roshani Codipilly
Master's Projects
With increases in space travel and a desire to inhabit the moon and Mars comes a pressing need to understand the impact of spaceflight on the body. Some effects are already known, such as reduced cardiac function and bone loss, but one area that needs to be further explored is the immune system. Differential gene expression analysis of mice thymi was performed to determine the impact of spaceflight on the immune system. The dataset that was analyzed, GLDS-289, was obtained from GeneLab, a space-omics database developed by NASA. Differential gene expression analysis was accomplished using a Nextflow implementation of GeneLab’s …
Differential Gene Expression Analysis Of Rodents Exposed To Long-Term Space Flight And Insights Into Physiological Effects, Jonathan Oribello
Differential Gene Expression Analysis Of Rodents Exposed To Long-Term Space Flight And Insights Into Physiological Effects, Jonathan Oribello
Master's Projects
Space travel presents inherent risks to human health and a better understanding of space biology is required to mitigate harm in an oncoming age of increased space travel. Omics analysis will play a central role in better understanding human health in space. In this project, differential gene expression analysis was performed on GLDS-104, an open science dataset provided by NASA’s GeneLab. GeneLab’s RNA-Seq Consensus Pipeline was implemented using Nextflow, performed on San Jose State University’s College of Science High Performance Computing Cluster, and optimized for computational resource efficiency. Comparison of the Nextflow implemention developed in this project to GeneLab’s posted …