Exploring Soil Microbial Dynamics In Southern Appalachian Forests: A Systems Biology Approach To Prescribed Fire Impacts, Saad Abd Ar Rafie

Doctoral Dissertations

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Prescribed fires in Southern Appalachian forests are vital in ecosystem management and wildfire risk mitigation. However, understanding the intricate dynamics between these fires, soil microbial communities, and overall ecosystem health remains challenging. This dissertation addresses this knowledge gap by exploring selected aspects of this complex relationship across three interconnected chapters.

The first chapter investigates the immediate effects of prescribed fires on soil microbial communities. It reveals subtle shifts in porewater chemistry and significant increases in microbial species richness. These findings offer valuable insights into the interplay between soil properties and microbial responses during the early stages following a prescribed fire. …

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Sources And Controls Of Carbon Dioxide In Inland Waters At Watershed, Regional, And Continental Scales, Brian Saccardi

Doctoral Dissertations

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Inland waters are significant sources of carbon dioxide to the atmosphere, and estimates of emissions are similar in magnitude to those of the carbon dioxide sequestered by the net terrestrial sink. Currently, methods of estimating carbon dioxide emissions are based on statistical approaches and often do not consider landscape attributes such as human development, agriculture, or the hydrologic connectivity of the stream network. The following research addresses these issues in chapter 1 by developing and validating a reactive transport model at the watershed scale, then in chapter 2 by applying the reactive transport model at the continental scale across US …

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Metagenomic Investigation Of Microbial Dark Carbon Fixation, Viral Interactions, And Horizonal Gene Transfer Within A Convergent Margin Subsurface Ecosystem, Timothy Joseph Rogers

Doctoral Dissertations

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Convergent margins are geological regions where two or more tectonic plates collide, and the denser “subducting slab” is pushed beneath the less dense overriding plate. As the slab descends, it devolatilizes under higher temperatures and pressures, allowing dissolved inorganic carbon (DIC) and redox active volatile rich fluids to cycle between the upper crust and Earth’s mantle. These fluids migrate through cracks and fissures in the upper mantle and crust, fueling chemolithoautotrophy-based microbial ecosystems in the subsurface before they are expelled on the surface in the form of hydrothermal seeps and springs. Chemolithoautotrophic ecosystems, such as those in the Costa Rican …

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Physiological Constraints, Mechanisms, And Mineral Transformations Of Iron Reduction In Hyperthermophilic Crenarchaea, Srishti Kashyap

Doctoral Dissertations

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Dissimilatory iron reduction by hyperthermophilic archaea occurs in many geothermal environments and typically relies on microbe-mineral interactions that transform various iron oxide minerals. However, the kinds of iron oxides that can be used, growth rates, extent of iron reduction, and the mineral transformations that occur due to this metabolism are poorly understood. This dissertation improves our fundamental understanding of the physiological mechanisms and mineral transformations of hyperthermophilic iron reduction using two model crenarchaea, Pyrodictium delaneyi and Pyrobaculum islandicum. Using growth yields and metabolite production rates, we demonstrated that a broad range of Fe(III) (oxyhydr)oxides of variable thermodynamic stability was …

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Fire-Vegetation-Climate Interactions Across The Holocene On The U.S. Southeastern Coastal Plain, Mathew S. Boehm

Doctoral Dissertations

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This dissertation research examined multiple proxy indicators in sediment cores from one lake and one wetland to reconstruct long-term relationships between fire, vegetation, and climate in the southeastern U.S.

At Lake Balboa (30.6992 N, 83.2031 W; 48 m elevation), a sinkhole pond located in southern Georgia, Bølling-Allerød conditions were sufficiently wet to maintain a shallow wetland at the site. Evidence for fire was minimal. Between 12,600 and 9200 cal yr BP, water availability declined, leading to a potential hiatus in sedimentation. During the early Holocene moisture availability increased, leading to greater primary productivity within and outside the lake, triggering an …

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Middle To Late Pleistocene Paleoenvironmental Reconstructions From Lake El'gygytgyn, Arctic Russia, Mary Helen Habicht

Doctoral Dissertations

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Climate change is a major issue challenging the world today. Our global society faces rising temperatures, variable weather patterns, and rising sea level among other associated issues. Our action (or inaction) to address current changes will have serious ramifications for life on our planet in the coming centuries and millennia. In order to provide context for these present and future changes, we can utilize the paleo record to understand the natural variability of Earth’s climate system. One region of the world is changing more rapidly than the global average. Over recent decades, the Arctic has experienced warmer temperatures, reduced sea …

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Sorption Temperature And And The Stability Of Iron-Bound Soil Organic Matter, Michael L. Nguyen

Doctoral Dissertations

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The preservation of soil organic matter (SOM) is an important control on the global cycling of carbon. Long-term preservation of SOM has important implications on soil fertility and climate regulation. Minerals, such as iron oxides, can react with SOM and serve as a preservation mechanism for SOM. Globally, iron oxide-SOM interactions form a “rusty carbon sink” which protects up to 22% of organic carbon in marine sediments. Climate changes, such as warming, may alter the size or efficacy of the “rusty carbon sink.” The effects of temperature, SOM composition, and mineral particle size on the formation and stability of iron …

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Wildfires In The Northeastern United States: Evaluating Fire Occurrence And Risk In The Past, Present, And Future, Daniel R. Miller

Doctoral Dissertations

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Climate change is one of the most complex and challenging issues facing the world today. A changing climate will affect humankind in many ways and alter our physical environment, presenting ethical challenges in how we respond. The impact of climate change will likely be exacerbated in heavily populated regions of the planet, such as the Northeastern United States (NEUS). The NEUS is comprised of complex, sprawling urban centers and rural regions, both of which are vital to the economic and cultural character of the region. Furthermore, both urban and rural areas in the NEUS contain communities that have been historically …

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Estimation Of Cdom In Inland Waters Via Water Bio-Optical Properties Using A Remote Sensing Approach, Jiwei Li

Doctoral Dissertations

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Monitoring of Colored dissolved organic matter (CDOM) in inland waters provides important information for tracing carbon cycle at the land-water interface and studying aquatic ecosystem. Remote sensing estimation of CDOM in the inland waters offers an alternative approach to field samplings in examining CDOM spatial-temporal dynamics. However, CDOM retrieval is a challenge due to the lack of algorithm for resolving bottom effect in shallow inland waters. Moreover, an effective approach based on multi-spectral, high spatial resolution and global coverage satellite images is in urgent need. To resolve these challenges, shallow water bio-optical properties (SBOP) algorithm was developed to overcome bottom …

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Arctic And North Atlantic Paleo-Environmental Reconstructions From Lake Sediments, Gregory A. De Wet

Doctoral Dissertations

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ABSTRACT

ARCTIC AND NORTH ATLANTIC PALEO-ENVIRONMENTAL RECONSTRUCTIONS FROM LAKE SEDIMENTS MAY 2017 GREGORY A. DE WET, B.Sc., BATES COLLEGE M.Sc., UNIVERSITY OF MASSSCHUSETTS, AMHERST Ph.D., UNIVERSITY OF MASSACHUSETTS, AMHERST Directed by: Drs. Raymond S. Bradley and Isla S. Castañeda There are few fields in the discipline of Earth Science that hold more relevancy in 2017 than studies of earth’s climate. Called the “perfect problem” considering its complexity and magnitude, climate change will continue to be one of the greatest challenges humanity will face in the 21^st century. And while numerical models provide valuable information on conditions in the future, …

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Biogeochemical Characteristics Of Organic Matter In A Karst Groundwater System, Teresa Lynn Brown

Doctoral Dissertations

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Development of regional groundwater carbon budgets hinges on the ability to quantify and monitor biogeochemical processes controlled by microbial recycling of natural dissolved organic matter (DOM) in carbon-limited (oligotrophic) areas. DOM is the major reservoir of organic carbon in aquatic ecosystems. However, isolation and characterization of DOM in oligotrophic freshwater systems has been limited by operational protocols and instrumentation. The goals of this research were to investigate the seasonal dynamics of microbially-driven organic matter degradation in a karst groundwater system influenced by surface water, and to identify analytical tools and biomarkers to measure long-term hydro-ecological trends in the Appalachian region. …

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Bacterial Diversity And Function Within An Epigenic Cave System And Implications For Other Limestone Cave Systems, Kathleen Merritt Brannen-Donnelly

Doctoral Dissertations

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There are approximately 48,000 known cave systems in the United States of America, with caves formed in carbonate karst terrains being the most common. Epigenic systems develop from the downward flow of meteoric water through carbonate bedrock and the solutional enlargement of interconnected subsurface conduits. Despite carbonate karst aquifers being globally extensive and important drinking water sources, microbial diversity and function are poorly understood compared to other Earth environments. After several decades of research, studies have shown that microorganisms in caves affect water quality, rates of carbonate dissolution and precipitation, and ecosystem nutrition through organic matter cycling. However, limited prior …

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Evidence Of Late Quaternary Fires From Charcoal And Siliceous Aggregates In Lake Sediments In The Eastern U.S.A., Joanne P. Ballard

Doctoral Dissertations

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The late-glacial transition to the Holocene, 15,000–11,600 cal yr BP, is an enigmatic period of dynamic global changes and a major extinction event in North America. Fire is an agent of disturbance that transforms the environment physically and chemically, and affects plant community composition. To improve understanding of the linkages between fire, vegetation, and climate over the late glacial and Holocene in the eastern U.S., I analyzed lake-sediment cores for charcoal and indicators of wood ash, and compared results to existing pollen records. A new microscopic charcoal record from Anderson Pond, Tennessee revealed high fire activity from 23,000–15,000 cal yr …

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Microbe-Mineral Relationships And Biogenic Mineral Transformations In Actively Venting Deep-Sea Hydrothermal Sulfide Chimneys, Tzihsuan J. Lin

Doctoral Dissertations

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This dissertation uses a combination of microbiology, mineralogy, and geochemistry to understand dissimilatory iron reduction in hyperthermophilic archaea and the role and potential impact of these and other vent microorganisms within active deep-sea hydrothermal vent chimneys. The central objective of the dissertation is to determine if mineral composition and chimney type are among the primary determinants of microbial community composition and hyperthermophilic, dissimilatory iron reducer growth, in addition to other environmental factors such as nutrient availability, temperature, pH, and chlorinity. This is done using samples and organisms collected from the Endeavour Segment of the Juan de Fuca Ridge in the …

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Genetic Analysis Of Bacterial Gene Variations In Sulfidic Springs And The Influence On Geochemistry, Brendan Joseph Headd

Doctoral Dissertations

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Culture-independent methods have revolutionized environmental microbiology and geomicrobiology studies and next-generation sequencing and metagenomics techniques continue to reveal the vast genetic diversity of microorganisms. But, these approaches provide comparatively little quantitative information about the roles that naturally occurring microbial gene variations play in local biogeochemical cycling. The goal of this study was to identify how the physical distribution and genetic diversity of microbial genes within a habitat impact environmental geochemistry by examining the biogeography of 16S rRNA genes and bacterial sulfur oxidation (Sox) genes in terrestrial sulfidic springs. 16S rRNA gene pyrosequences were obtained from microbial mats inhabiting eight sulfidic …

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Mechanisms Of Nutrient Limitation And Nutrient Acquisition In Managed And Unmanaged Forest Ecosystems, Matthew A. Vadeboncoeur

Doctoral Dissertations

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Understanding the interactions between global change, human and natural disturbances, and other factors on biogeochemical processes in forests is necessary to ensure the sustainability of forest management. Here I report the results of several investigations into nutrient acquisition processes in the forests of New Hampshire. I begin with a meta-analysis of fertilization studies showing that phosphorus (P) and calcium (Ca) as well as nitrogen (N) may limit primary production in deciduous forests of the region. Because these limiting nutrients are all removed from the ecosystem when trees are harvested, I compared nutrient budgets under a range of harvesting scenarios with …

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