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Ca2+ - Induced Structural Change Of Multi-Domain Collagen Binding Segments Of Collagenases Colg And Colh From Hathewaya Histolytica, Christopher Eric Ruth
Ca2+ - Induced Structural Change Of Multi-Domain Collagen Binding Segments Of Collagenases Colg And Colh From Hathewaya Histolytica, Christopher Eric Ruth
Graduate Theses and Dissertations
Hathewaya histolytica, previously renamed as Clostridium histolyticum, secretes at least two collagenases, ColH and ColG, that allow for degradation of extracellular matrices of animal tissue. Hathewaya histolytica virulence factors are proposed to undergo domain rearrangement upon secretion from the bacteria to the host. In order to accomplish this, collagenases seek the least ordered regions in collagen to efficiently disassemble the fibril. Two types of domains, Polycystic Kidney Disease-like (PKD) and Collagen Binding Domain (CBD), direct the collagenases ability to disassemble the fibril. Calcium dependent structural change have been reported to increase in thermal stability and in tighter collagen binding for …