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- Aerobiological index (1)
- Calcium alginate; Enzyme entrapment; Enzyme reusability; Enzyme stability; Lipase (1)
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- Infrared; grapes; composition; viticulture; chemometrics (1)
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- Met exon 14 skipping; diagnostic assay (1)
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- Oesophageal cancer (1)
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- Streptomyces griseus; recombinant β-glucosidase; Fractional Factorial design Plackett-Burman Design; Definitive Screening Design; Response Surface Methodology (1)
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- Urban planning (1)
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Articles 1 - 9 of 9
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The Effect Of Calcium Alginate Entrapment On The Stability Of Novel Lipases From P. Reinekei And P. Brenneri, Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan
The Effect Of Calcium Alginate Entrapment On The Stability Of Novel Lipases From P. Reinekei And P. Brenneri, Priyanka Priyanka, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan
Articles
The high cost of soluble enzymes can limit their use for commercial and industrial purposes. Immobilization can enhance enzyme reusability, thereby reducing product isolation costs and overcoming this economic barrier. In the current study, two novel, purified lipases from Pseudomonas sp. (Pseudomonas reinekei and Pseudomonas brenneri) were entrapped in a calcium alginate matrix, with the aim of simultaneously enhancing enzyme reusability and stability. Following entrapment, the retained activity of the enzyme-alginate composite was verified by an enzymatic hydrolysis reaction of a p-nitrophenol palmitate substrate. The effect of the enzyme-alginate entrapment against various physiochemical parameters such as pH, temperature, metal ions, …
Cold Atmospheric Plasma Induces Accumulation Of Lysosomes And Caspase-Independent Cell Death In U373mg Glioblastoma Multiforme Cells, Gillian Conway, Zhonglei He, Ana L. Hutanu, George P. Cribaro, Eline Manaloto, Alan Casey, Damien Traynor, Vladimir Milosavljevic, Orla Howe, Carlos Barcia, James T. Murray, Patrick Cullen, James Curtin
Cold Atmospheric Plasma Induces Accumulation Of Lysosomes And Caspase-Independent Cell Death In U373mg Glioblastoma Multiforme Cells, Gillian Conway, Zhonglei He, Ana L. Hutanu, George P. Cribaro, Eline Manaloto, Alan Casey, Damien Traynor, Vladimir Milosavljevic, Orla Howe, Carlos Barcia, James T. Murray, Patrick Cullen, James Curtin
Articles
Room temperature Cold Atmospheric Plasma (CAP) has shown promising efficacy for the treatment of cancer but the exact mechanisms of action remain unclear. Both apoptosis and necrosis have been implicated as the mode of cell death in various cancer cells. We have previously demonstrated a caspase-independent mechanism of cell death in p53-mutated glioblastoma multiforme (GBM) cells exposed to plasma. The purpose of this study was to elucidate the molecular mechanisms involved in caspase-independent cell death induced by plasma treatment. We demonstrate that plasma induces rapid cell death in GBM cells, independent of caspases. Accumulation of vesicles was observed in plasma …
The Statistical Optimisation Of Recombinant Β-Glucosidase Production Through A Two-Stage, Multi-Model, Design Of Experiments Approach, Albert Uhoraningoga, Gemma K. Kinsella, Jesus Maria Frias, Gary T. Henehan, Barry J. Ryan
The Statistical Optimisation Of Recombinant Β-Glucosidase Production Through A Two-Stage, Multi-Model, Design Of Experiments Approach, Albert Uhoraningoga, Gemma K. Kinsella, Jesus Maria Frias, Gary T. Henehan, Barry J. Ryan
Articles
β-glucosidases are a class of enzyme that are widely distributed in the living world, with examples noted in plants, fungi, animals and bacteria. They offer both hydrolysis and synthesis capacity for a wide range of biotechnological processes. However, the availability of native, or the production of recombinant β-glucosidases, is currently a bottleneck in the widespread industrial application of this enzyme. In this present work, the production of recombinant β-glucosidase from Streptomyces griseus was optimised using a Design of Experiments strategy, comprising a two-stage, multi-model design. Three screening models were comparatively employed: Fractional Factorial, Plackett-Burman and Definitive Screening Design. Four variables …
Urban Aerobiological Risk Mapping Of Ornamental Trees Using A New Index Based On Lidar And Kriging: A Case Study Of Plane Trees, Raúl Pecero-Casimiro, Santiago Fernández-Rodríguez, Rafael Tormo-Molina, Alejandro Monroy-Colín, Inmaculada Silva-Palacios, Juan Pedro Cortés-Pérez, Ángela Gonzalo-Garijo, J. M. Maya-Manzano
Urban Aerobiological Risk Mapping Of Ornamental Trees Using A New Index Based On Lidar And Kriging: A Case Study Of Plane Trees, Raúl Pecero-Casimiro, Santiago Fernández-Rodríguez, Rafael Tormo-Molina, Alejandro Monroy-Colín, Inmaculada Silva-Palacios, Juan Pedro Cortés-Pérez, Ángela Gonzalo-Garijo, J. M. Maya-Manzano
Articles
Ornamental trees bring benefits for human health, including reducing urban pollution. However, some species, such as plane trees (Platanus sp.), produce allergenic pollen. Consequently, urban maps are a valuable tool for allergic patients and allergists, but they often fail to include variables that contribute to the “building downwash effect”, such as the width and shape of streets and the height of buildings. Other factors that directly influence pollen dispersion (slopes and other geographical features) also have not traditionally been discussed. The LiDAR (Laser Imaging Detection and Ranging) technique enables one to consider these variables with high accuracy. This work proposes …
From The Laboratory To The Vineyard—Evolution Of The Measurement Of Grape Composition Using Nir Spectroscopy Towards High-Throughput Analysis, Aoife Power, Vi Khanh Truong, James Chapman, Daniel Cozzolino
From The Laboratory To The Vineyard—Evolution Of The Measurement Of Grape Composition Using Nir Spectroscopy Towards High-Throughput Analysis, Aoife Power, Vi Khanh Truong, James Chapman, Daniel Cozzolino
Articles
Compared to traditional laboratory methods, spectroscopic techniques (e.g., near infrared, hyperspectralimaging)provideanalystswithaninnovativeandimprovedunderstandingofcomplex issuesbydeterminingseveralchemicalcompoundsandmetabolitesatonce,allowingforthecollection of the sample “fingerprint”. These techniques have the potential to deliver high-throughput options for the analysis of the chemical composition of grapes in the laboratory, the vineyard and before or during harvest, to provide better insights of the chemistry, nutrition and physiology of grapes. Faster computers, the development of software and portable easy to use spectrophotometers and data analytical methods allow for the development of innovative applications of these techniques for the analyses of grape composition.
Measuring The Impact Of Oesophagectomy On Physical Functioning And Physical Activity Participation: A Prospective Study, E.M. Guinan, A.E. Bennett, Suzanne Doyle, Luke O'Neill, J. Gannon, G. Foley, J.A. Elliott, J. O'Sullivan, J.V. Reynolds, J. Hussey
Measuring The Impact Of Oesophagectomy On Physical Functioning And Physical Activity Participation: A Prospective Study, E.M. Guinan, A.E. Bennett, Suzanne Doyle, Luke O'Neill, J. Gannon, G. Foley, J.A. Elliott, J. O'Sullivan, J.V. Reynolds, J. Hussey
Articles
Background: Oesophagectomy remains the only curative intervention for oesophageal cancer, with defined nutritional and health-related quality of life (HR-QOL) consequences. It follows therefore that there is a significant risk of decline in physical wellbeing with oesophagectomy however this has been inadequately quantified. This study prospectively examines change in physical functioning and habitual physical activity participation, from presurgery through 6-months post-oesophagectomy. Methods: Patients scheduled for oesophagectomy with curative intent were recruited. Key domains of physical functioning including exercise tolerance (six-minute walk test (6MWT)) and muscle strength (hand-grip strength), and habitual physical activity participation, including sedentary behaviour (accelerometry) were measured presurgery (T0) …
Decadal Biomass Increment In Early Secondary Succession Woody Ecosystems Is Increased By Co2 Enrichment, Anthony Walker, Martin G. De Kauwe, Blinda E. Medlyn, Sonke Zaehle, Colleen M. Iversen, Shinichi Asao, Bertrand Guenet, Anna Harper, Tonas Hickler, Bruce A. Hungate, Atul K. Jain, Yiqi Luo, Xingjie Lu, Meng Lu, Kristina Luus, J. Patrick Megonigal, Ram Oren, Edmund Ryan, Shijie Shu, Alan Talhelm, Ying-Ping Wang, Jeffrey M. Warren, Christian Werner, Jianyang Xia, Bai Yang, Donald R. Zak, Richard J. Norby
Decadal Biomass Increment In Early Secondary Succession Woody Ecosystems Is Increased By Co2 Enrichment, Anthony Walker, Martin G. De Kauwe, Blinda E. Medlyn, Sonke Zaehle, Colleen M. Iversen, Shinichi Asao, Bertrand Guenet, Anna Harper, Tonas Hickler, Bruce A. Hungate, Atul K. Jain, Yiqi Luo, Xingjie Lu, Meng Lu, Kristina Luus, J. Patrick Megonigal, Ram Oren, Edmund Ryan, Shijie Shu, Alan Talhelm, Ying-Ping Wang, Jeffrey M. Warren, Christian Werner, Jianyang Xia, Bai Yang, Donald R. Zak, Richard J. Norby
Articles
Increasing atmospheric CO2 stimulates photosynthesis which can increase net primary production (NPP), but at longer timescales may not necessarily increase plant biomass. Here we analyse the four decade-long CO2-enrichment experiments in woody ecosystems that measured total NPP and biomass. CO2 enrichment increased biomass increment by 1.05 ± 0.26 kg C m−2 over a full decade, a 29.1 ± 11.7% stimulation of biomass gain in these early-secondary-succession temperate ecosystems. This response is predictable by combining the CO2 response of NPP (0.16 ± 0.03 kg C m−2 y−1) and the CO2-independent, linear slope between biomass increment and cumulative NPP (0.55 ± 0.17). …
Cost-Efficient And Easy To Perform Pcr-Based Assay To Identify Met Exon 14 Skipping In Formalin-Fixed Paraffin-Embedded (Ffpe) Non-Small Cell Lung Cancer (Nsclc) Samples, Odharnaith O'Brien, Mark C. Wright, Cathal O'Brien, Orla Geoghegan, Niamh Leonard, Siobhan Nicolson, Sinead Cuffe, Aurelie Fabre, Wolfram Jochum, Markus Joerger, Steven G. Gray, Stephen P. Finn
Cost-Efficient And Easy To Perform Pcr-Based Assay To Identify Met Exon 14 Skipping In Formalin-Fixed Paraffin-Embedded (Ffpe) Non-Small Cell Lung Cancer (Nsclc) Samples, Odharnaith O'Brien, Mark C. Wright, Cathal O'Brien, Orla Geoghegan, Niamh Leonard, Siobhan Nicolson, Sinead Cuffe, Aurelie Fabre, Wolfram Jochum, Markus Joerger, Steven G. Gray, Stephen P. Finn
Articles
MET is a receptor tyrosine kinase (RTK) that plays important roles in carcinogenesis. Despite being frequently overexpressed in cancer, clinical responses to targeting this receptor have been limited. Recently novel splicing mutations involving the loss of exon 14 (called METex14 skipping) have emerged as potential biomarkers to predict for responsiveness to targeted therapies with Met inhibitors in non-small cell lung cancer (NSCLC). Currently, the diverse genomic alterations responsible for METex14 skipping pose a challenge for routine clinical diagnostic testing. In this report, we examine three different methodologies to detect METex14 and assess their potential utility for use as a diagnostic …
The Match-Play Sprint Performance Of Elite Senior Hurlers During Competitive Games, Damien Young, Giuseppe Coratella, Shane Malone, Kieran Collins, Laurent Mourot, Marco Beato
The Match-Play Sprint Performance Of Elite Senior Hurlers During Competitive Games, Damien Young, Giuseppe Coratella, Shane Malone, Kieran Collins, Laurent Mourot, Marco Beato
Articles
The typical sprint profile in elite hurling has yet to be established. The purpose of this study was to investigate the sprinting demands of elite hurling competition and characterize the sprinting patterns of different playing positions. GPS (10-Hz, STATSports Viper) were used to collect data from 51 hurlers during 18 games. The total sprint (≥22 km·h-1) distance (TSD), the number of sprints (NOS) classified as length (<20 >m, ≥20 m) and relative speed thresholds (90%), the between-sprint duration and the number of repeated-sprint bouts (≥2 sprints in ≤60 s) were analyzed. The NOS was 22.2 ± 6.8 accumulating …