Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Entire DC Network
Down-Regulation Of The Caffeic Acid O-Methyltransferase Gene In Switchgrass Reveals A Novel Monolignol Analog, Timothy J. Tschaplinski, Robert F. Standaert, Nancy L. Engle, Madhavi Z. Martin, Amandeep K. Sangha, Jerry M. Parks, Jeremy C. Smith, Reichel Samuel, Nan Jiang, Yunqiao Pu, Arthur J. Ragauskas, Choo Y. Hamilton, Chunxiang Fu, Zeng-Yu Wang, Brian H. Davidson, Richard A. Dixon, Jonathan R. Mielenz
Down-Regulation Of The Caffeic Acid O-Methyltransferase Gene In Switchgrass Reveals A Novel Monolignol Analog, Timothy J. Tschaplinski, Robert F. Standaert, Nancy L. Engle, Madhavi Z. Martin, Amandeep K. Sangha, Jerry M. Parks, Jeremy C. Smith, Reichel Samuel, Nan Jiang, Yunqiao Pu, Arthur J. Ragauskas, Choo Y. Hamilton, Chunxiang Fu, Zeng-Yu Wang, Brian H. Davidson, Richard A. Dixon, Jonathan R. Mielenz
Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology
Background
Down-regulation of the caffeic acid 3-O-methyltransferase EC 2.1.1.68 (COMT) gene in the lignin biosynthetic pathway of switchgrass (Panicum virgatum) resulted in cell walls of transgenic plants releasing more constituent sugars after pretreatment by dilute acid and treatment with glycosyl hydrolases from an added enzyme preparation and from Clostridium thermocellum. Fermentation of both wild-type and transgenic switchgrass after milder hot water pretreatment with no water washing showed that only the transgenic switchgrass inhibited C. thermocellum. Gas chromatography–mass spectrometry (GCMS)-based metabolomics were undertaken on cell wall aqueous extracts to determine the nature of the microbial …