Digital Commons Network^™

The Effect Of Metabolites, Which Accumulate During Phenylketonuria, On The Activity Of Stearoyl-Coenzyme A Desaturase, Walter Scott

Masters Theses

No abstract provided.

Comparison Of Protein Kinase Activity In Tissue Extracts Of The Diabetic And Nondiabetic Chinese Hamster, Michael Andrew Dombos

Masters Theses

No abstract provided.

The Half-Life Of Bacterial Messenger Rna, John B. Madison

Masters Theses

No abstract provided.

Isolation, Purification And Partial Characterization Of Some Isoenzymes Of Human Serum Alkaline Phosphatase, M. Sue Jones

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by M. Sue Jones on July 18, 1977.

Limnological Aspects Of Lake Mead, Nevada-Arizona, John R. Baker, James E. Deacon, Thomas A. Burke, Samuel S. Egdorf, Larry J. Paulson, Richard W. Tew, Bureau Of Reclamation

Publications (WR)

Lake Mead is a deep, subtropical, moderately productive, desert impoundment with a negative heterograde oxygen profile occurring during; the summer stratification. investigations of the Boulder Basin of Lake Mead by the University of Nevada were initiated in November 1971. The primary objective of the study was to determine what effects industrial and sewage effluent from the Las Vegas metropolitan area, discharged into Las Vegas Bay, have had on the water quality and limnological conditions of Boulder Basin. Data from the 1975-76 period are presented in detail, with earlier data included in the summaries and discussions.

Measurements of water temperature, dissolved …

Light Scattering Studies Of Transverse Sound Wave And Molecular Motion In Benzonitrile, Scott L. Whittenburg, C. H. Wang

Chemistry and Biochemistry Faculty Publications

The zero‐frequency shear wave dip appearing in the depolarized Rayleigh spectrum in benzonitrile has been studied as a function of concentration and temperature. The solution study was carried out at constant viscosity equal to the viscosity of liquid benzonitrile at each temperature. The result indicates that the presence of shear wave fine structure does not depend on the collective orientational fluctuations. The orientational and vibrational relaxation times of benzonitrile were measured at various concentrations and temperatures. The orientational relaxation times show no concentration dependence at any temperature, suggesting that the pair correlation is negligible at all concentrations. The orientational relaxation …

Human Placental Alkaline Phosphatase And Steroid Metabolism By Rat Adrenals, Allen G. Meier

Loma Linda University Electronic Theses, Dissertations & Projects

Human placental alkaline phosphatase was purified by butanol extraction, methanol precipitation and chromatography on DEAE-cellulose. The pH optimum was found to be 10.9. The enzyme was competitively inhibited by di-ethyl-p-nitrobenzyl-phosphonate and copper sulfate. An increase in enzymatic activity occurred in the presence of the phosphate ion (HPO₄) and ATP.

The enzyme was crystallized from a 40% saturated solution of ammonium sulfate and shown to be homogeneous by sedimentation analysis. Homogeneity of the enzyme was also demonstrated using poly-acrylamide-gel-electrophoresis. At a pH of 7.5% the electrophoretic pattern yielded a single band and showed no significant contamination.

The metabolic transformation …

The Relationship Of Reduced Levels Of Atp To Inhibited Chondrogenesis In The Production Of Limb Deformity Induced By 6-Aminonicotinamide, Val C. Sheffield

Theses and Dissertations

6-aminonicotinamide (6-AN), an analogue and antagonist of nicotinamide, impairs cartilage formation and results in shortening of the limb when administered to chick embryos. 6-AN forms an abnormal NAD analogue (6-ANAD), which inhibits the activity of NAD-dependent enzymes associated with the production of ATP. The mechanism of action of 6-AN was studied by measuring the biosynthesis of protein and DNA, and the sulfation and glycosylation of chondroitin sulfate in control and 6-AN treated cartilage from 8-day chick embryos. The cartilage was also assayed for ATP levels. Incorporation of Na235SO4 was inhibited by 6 h treatment with 10 μg/ml 6-AN, whereas 3H-thymidine …

Light Scattering Studies Of Rotational And Vibrational Relaxations Of Acetonitrile In Carbon Tetrachloride, Scott L. Whittenburg, C. H. Wang

Chemistry and Biochemistry Faculty Publications

The rotational and vibrational relaxation times of acetonitrile–carbon tetrachloride solutions were investigated as a function of concentration, viscosity, and temperature using depolarized Rayleigh and Raman scattering. Using a Fabry‐Perot interferometer and single frequency laser source, we have shown that reliable results for the single particle orientational correlation times (τ_s) for CH₃CN can be obtained by carrying out a concentration dependent depolarized Rayleigh scattering study. Raman scattering was shown to yield inconsistent results for τ_s in CH₃CN. At constant viscosity, it was found that the Rayleigh scatteringrelaxation time (τ_Ray) of CH_{3 …}

Metabolic Regulation Of Glycolate Synthesis, Photorespiration, And Net Photosynthesis In Tobacco By L-Glutamate, David J. Oliver, Israel Zelitch

David J. Oliver

Experiments were undertaken to identify and characterize control mechanisms in tobacco leaf tissue which decrease the relative contribution of photorespiratory CO2 release and thereby increase net photosynthetic CO2 fixation. A number of metabolites were supplied to illuminated leaf discs and their effect on the inhibition of glycolate synthesis was measured. Glycolate accumulation, in the presence of α-hydroxy-2-pyridinemethanesulfonic acid, was inhibited in leaf discs previously floated on 30 mM solutions of either L-glutamate, L-aspartate, phospho-enolpyruvate, or glyoxylate. The effect of glutamate on glycolate synthesis, which was investigated in detail, was concentration- and time-dependent. Glycolate synthesis was inhibited about 40% by treating …

Isolation, Purification, Scanning Electron Microscopy And Bacterial Dna Uptake Of Plant Protoplasts, Bronwyn G. Hughes

Theses and Dissertations

Protoplasts were isolated from tobacco and barley leaves in sucrose or mannitol using commercially available cellulases and macerozymes. Barley growth and protoplast isolation and purification conditions were optimized so that protoplasts were obtained in high yields free of unwanted debris and organelles. A technique for processing barley and tobacco protoplasts for examination by scanning electron microscopy was developed in which protoplasts seem to have maintained their structural integrity. Barley and tobacco protoplasts took up 3H-B. subtilis DNA, 125I-B. subtilis DNA or 125I-M. luteus DNA as a linear function of time (0-6 hr) and DNA concentration (0-200 μg/ml). Up to 16 …

Covalent Linkage Of A Protein To A Defined Nucleotide Sequence At The 5'-Terminus Of Virion And Replicative Intermediate Rnas Of Poliovirus, James B. Flanegan, Ralf F. Pettersson, Victor R. Ambros, Martinez J. Hewlett, David Baltimore

Victor R. Ambros

The 5'-terminus of poliovirus polyribosomal RNA is pUp. A candidate for the 5'-terminus of poliovirion RNA was recovered as a compound migrating toward the cathode when 32P-labeled virion RNA was completely digested with ribonucleases T1, T2 and A and analyzed by paper ionophoresis at pH 3.5. Treatment with proteinase K reversed its direction of migration, indicating the presence of protein. Treatment with venom phosphodiesterase liberated all of the radioactivity as pUp, suggesting that poliovirion RNA has a protein-pUp 5'-terminus. Treatment of virion RNA with T1 ribonuclease alone generated a proteinase K-sensitive oligoribonucleotide. Analysis of the oligoribonucleotide using ribonucleases A and …

Intermolecular Potential Surfaces From Electron Gas Methods. Ii. Angle And Distance Dependence Of The A’ And A” Ar–No(X^2ii) Interactions, Glen C. Nielson, Gregory A. Parker, Russell T. Pack

Faculty Publications

Angle dependent intermolecular potential energy surfaces for the two states (2A’ and 2A”) that arise from the interaction of ground (X^2II) state NO with Ar are calculated using the electron gas model to obtain the short range interactions. The average and difference of the two interaction energies are fit to analytic forms convenient for use in scattering calculations and joined smoothly onto the long range van der Waals potential previously determined. The results, which appear to be of useful accuracy, and the applicability of the electron gas model to such open shell–closed shell interactions are discussed.

Evidence For Chromium-Insulin Interaction, Ronald Belusko

Dissertations

No abstract provided.

Changes In Morphology And Protein Content Of Neurons And Glia Of The Developing Hamster Cerebral Cortex, Thomas E. Durica

Dissertations

No abstract provided.

Studies On The Mechanism Of Inhibition Of Rna Synthesis By Template Inactivators, Nikos Panayotatos

Dissertations

No abstract provided.

Characterization Of Carbohydrates In Oyster (Crassostrea Virginica) Hemolymph, Robert Joel Lowy

Dissertations, Theses, and Masters Projects

No abstract provided.

Adaptive Mechanisms For Aquatic Existence In Freshwater Turtles, Oscar Gloor

Honors Theses

Naturalists have often marveled at the ability of some air-breathing vertebrates to remain underwater for long periods of time (that is, "long" from man's reckoning). Seals, penguins, porpoises, and whales are all noted fro their ability to use oxygen stored in various ways to permit them to "stay under" for many minutes.

The return of vertebrates, which evolved on land, to an aquatic existence has been a much discussed subject from an evolutionary standpoint. That there are many advantages to life underwater is easily seen simply by the fact that, according to fossil records, terrestrial life arose from the sea. …

Direct Chemical Evidence For A Supramolecular Protein Structure In The Nuclear Envelope, David Lee Cochran

Theses and Dissertations

Protein subunit structure in the nuclear envelope has not been previously described. Covalent crosslinking of polypeptides provides a method for studying the subunit structure of protein-rich systems. In this study, several polypeptide crosslinking methods have been used to determine associations among polypeptides in isolated chicken erythrocyte nuclear envelope. Interchain associations that exist in the isolated membrane were fixed by crosslinking the polypeptides in the isolated nuclear envelope. Crosslinked and uncrosslinked polypeptides were resolved by electrophoresis in a dissociating detergent system. Each agent caused distinctive alterations in the gel pattern. Certain bands diminished and disappeared while new bands of two or …