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Complex Regulatory Pathways Connect Vfm Quorum-Sensing And Cyclic-Di-Gmp Signaling To Bacterial Virulence In The Phytopathogen Dickeya Dadantii 3937, Biswarup Banerjee

Theses and Dissertations

The bacterial second messenger Bis-(3’-5’)-cyclic dimeric guanosine monophosphate (c-di-GMP) regulates multiple cellular behaviors in most bacteria. Bacterial c-di-GMP signaling involves enzymes that synthesize and degrade c-di-GMP, c-di-GMP binding effectors, and targets that are acted upon by the effectors. So far, the c-di-GMP signaling pathways have been understudied. In this work, we explore the c-di-GMP signaling network further in the phytopathogen Dickeya dadantii 3937. In Chapter 2, we identified VfmE as a c-di-GMP binding transcriptional activator that represses pectate lyase production under a high c-di-GMP condition. VfmE was found to bind c-di-GMP in vitro via the RxxxR motif, similar to PilZ …