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Translation Of Partially Decayed Messenger Rnas In Yeast, Ana Luisa Franklin
Translation Of Partially Decayed Messenger Rnas In Yeast, Ana Luisa Franklin
Electronic Theses and Dissertations
Flaviviruses are positive-strand single-stranded RNA viruses that are known to form pseudo-knot RNA structures that halt the progression of 5’→3’ exonuclease Xrn1. We show that these viral Xrn1-resistant structures (xrRNAs) can be used to protect specific homologously-expressed messenger RNAs from 5’→3’ degradation. We investigated the effects of addition of xrRNAs, artificially-installed into the intergenic region of bicistronic mRNA reporters, in the observed levels of protein expression in yeast. The reporters also contain an internal ribosome entry site from the cricket paralysis virus (CrPV IRES) to allow for cap-independent translation of the decay-protected gene, LacZ, encoding the enzyme β-galactosidase. Through …
Development Of A Hek293 Cell Line To Show Inhibition Of Tau Aggregation, Justin Ray Shady
Development Of A Hek293 Cell Line To Show Inhibition Of Tau Aggregation, Justin Ray Shady
Electronic Theses and Dissertations
Intracellular deposition of aggregated tau is the hallmark of several different tauopathies, the most widespread of these being Alzheimer's disease. Tau is a highly soluble, intrinsically disordered, microtubule associated protein. Tau's native function is to stabilize microtubule formation in the axons of neurons. Post translational modification such as hyperphosphorylation as well as several familial mutations allow tau to nucleate and form fibrils. These fibrils can recruit healthy monomers onto their ends in a fashion described as template-assisted growth. Tau has 6 isoforms that vary by the inclusion or exclusion of two N-terminal repeats and the inclusion or exclusion of the …
Mechanistic Insight Into Tau Fibril Cross-Seeding Barriers: Structural Order And Disorder, Hilary Ann Weismiller
Mechanistic Insight Into Tau Fibril Cross-Seeding Barriers: Structural Order And Disorder, Hilary Ann Weismiller
Electronic Theses and Dissertations
Filamentous deposition of microtubule-associated protein tau is a hallmark for a number of neurodegenerative diseases collectively termed tauopathies. While tau fibrils are directly linked to the etiology and pathogenesis of these diseases, fibril morphology and their phenotypic presentation can be quite disparate. Alternative splicing of tau results in two main isoform groups, four-repeat (4R) tau and three-repeat (3R), identified by their number of microtubule binding repeats. Some diseases show deposition of both 4R and 3R isoforms, while others show preferential deposition of only one type. The conformational templated growth scheme and trans-synaptic spreading of fibrils is influenced by fibril conformation. …