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Affinity Purification And Characterization Of E. Coli Molecular Chaperones, Seung-Hee Nam
Affinity Purification And Characterization Of E. Coli Molecular Chaperones, Seung-Hee Nam
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The molecular chaperones are a group of proteins that are effective in vitro and in vivo folding aids and show a well documented affinity for proteins lacking tertiary structure.
Heat-induced Escherichia coli BL21 cell lysate (10 mg protein) was applied to immobilized ɑ-casein (45 mg/g beads) or β-casein (30 mg/g beads) column. After removing a majority of nonspecifically bound proteins with 1 M NaCl, the molecular chaperones were eluted with cold water, 1 mM Mg-ATP, or 6 M urea. Western analysis identified five Escherichia coli molecular chaperones including DnaK, DnaJ, GrpE, GroEL, and GroES. Among samples, ATP eluates showed the …