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The Cost Of Mupirocin Resistance In Staphylococcus., Susan D. Reynolds
The Cost Of Mupirocin Resistance In Staphylococcus., Susan D. Reynolds
Electronic Theses and Dissertations
Control of antibiotic resistance in bacteria is based on the concept that resistance incurs a fitness cost in non-selective conditions. Fitness costs were assessed for low- and high-level mupirocin resistance in locally-derived Staphylococcus aureus and S. epidermidis. Costs of resistance were assessed in pure cultures by comparing growth curve characteristics and in mixed culture as the proportion of resistant cells surviving. Costs were not present in comparisons of growth rates among groups of naturally-occurring isolates from the different resistance categories. However, in S. aureus, growth rates within resistance categories differed by approximately 30 – 90%. Among near-isogenic pairs …
Conjugative Transfer Pathways Of High-Level Mupirocin Resistance And Conjugative Transfer Genes In Staphylococcus., Danielle Barnard
Conjugative Transfer Pathways Of High-Level Mupirocin Resistance And Conjugative Transfer Genes In Staphylococcus., Danielle Barnard
Electronic Theses and Dissertations
To combat widespread infections caused by Staphylococcus aureus, mupirocin was introduced at the Veterans Affairs Medical Center, Mountain Home, Tennessee. Soon after introduction, high-level mupirocin-resistance emerged. The rapid emergence was hypothesized to be due to conjugative transfer of the mupA resistance gene from S. epidermidis to S. aureus. Results have shown that transfer of high-level mupirocin-resistance from S. aureus donors commonly occurs. However, transfer from naturally-occurring S. epidermidis donors was not attainable. Staphylococcus epidermidis transconjugants, however, were capable of serving as donors. Further examination of non-transmissibility included PCR analysis of conjugative transfer genes (tra genes) in capable …