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Improved Automated Monitoring And New Analysis Algorithm For Circadean Phototaxis Rhythms In Chlamydomonas, Christa Gaskill, Jennifer Forbes-Stovall, Bruce Kessler, Mike Young, Claire A. Rinehart, Sigrid Jacobshagen
Improved Automated Monitoring And New Analysis Algorithm For Circadean Phototaxis Rhythms In Chlamydomonas, Christa Gaskill, Jennifer Forbes-Stovall, Bruce Kessler, Mike Young, Claire A. Rinehart, Sigrid Jacobshagen
Bruce Kessler
Automated monitoring of circadian rhythms is an efficient way of gaining insight into oscillation parameters like period and phase for the underlying pacemaker of the circadian clock. Measurement of the circadian rhythm of phototaxis (swimming towards light) exhibited by the green alga Chlamydomonas reinhardtii has been automated by directing a narrow and dim light beam through a culture at regular intervals and determining the decrease in light transmittance due to the accumulation of cells in the beam. In this study, the monitoring process was optimized by constructing a new computercontrolled measuring machine that limits the test beam to wavelengths reported …